Universal Markers Unveil Metastatic Cancerous Cross-Sections at Nanoscale
The characterization of cancer histological sections as metastatic, M, or not-metastatic, NM, at the cellular size level is important for early diagnosis and treatment. We present timely warning markers of metastasis, not identified by existing protocols and used methods. Digitized atomic force micr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/15/3728 |
| _version_ | 1797414431527796736 |
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| author | Evangelos Bakalis Angelo Ferraro Vassilios Gavriil Francesco Pepe Zoe Kollia Alkiviadis-Constantinos Cefalas Umberto Malapelle Evangelia Sarantopoulou Giancarlo Troncone Francesco Zerbetto |
| author_facet | Evangelos Bakalis Angelo Ferraro Vassilios Gavriil Francesco Pepe Zoe Kollia Alkiviadis-Constantinos Cefalas Umberto Malapelle Evangelia Sarantopoulou Giancarlo Troncone Francesco Zerbetto |
| author_sort | Evangelos Bakalis |
| collection | DOAJ |
| description | The characterization of cancer histological sections as metastatic, M, or not-metastatic, NM, at the cellular size level is important for early diagnosis and treatment. We present timely warning markers of metastasis, not identified by existing protocols and used methods. Digitized atomic force microscopy images of human histological cross-sections of M and NM colorectal cancer cells were analyzed by multifractal detrended fluctuation analysis and the generalized moments method analysis. Findings emphasize the multifractal character of all samples and accentuate room for the differentiation of M from NM cross-sections. Two universal markers emphatically achieve this goal performing very well: (a) the ratio of the singularity parameters (left/right), which are defined relative to weak/strong fluctuations in the multifractal spectrum, is always greater than 0.8 for NM tissues; and (b) the index of multifractality, used to classify universal multifractals, points to log-normal distribution for NM and to log-Cauchy for M tissues. An immediate large-scale screening of cancerous sections is doable based on these findings. |
| first_indexed | 2024-03-09T05:32:56Z |
| format | Article |
| id | doaj.art-188f368055bb42afa58ef81af8ae213d |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T05:32:56Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-188f368055bb42afa58ef81af8ae213d2023-12-03T12:31:04ZengMDPI AGCancers2072-66942022-07-011415372810.3390/cancers14153728Universal Markers Unveil Metastatic Cancerous Cross-Sections at NanoscaleEvangelos Bakalis0Angelo Ferraro1Vassilios Gavriil2Francesco Pepe3Zoe Kollia4Alkiviadis-Constantinos Cefalas5Umberto Malapelle6Evangelia Sarantopoulou7Giancarlo Troncone8Francesco Zerbetto9Dipartimento di Chimica “G. Ciamician”, Università di Bologna, 2 Via Francesco Selmi, 40126 Bologna, ItalyTheoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, GreeceTheoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, GreeceDipartimento di Sanità Pubblica, Università degli Studi di Napoli “Federico II”, 5 Via Pansini, 801301 Napoli, ItalyTheoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, GreeceTheoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, GreeceDipartimento di Sanità Pubblica, Università degli Studi di Napoli “Federico II”, 5 Via Pansini, 801301 Napoli, ItalyTheoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, GreeceDipartimento di Sanità Pubblica, Università degli Studi di Napoli “Federico II”, 5 Via Pansini, 801301 Napoli, ItalyDipartimento di Chimica “G. Ciamician”, Università di Bologna, 2 Via Francesco Selmi, 40126 Bologna, ItalyThe characterization of cancer histological sections as metastatic, M, or not-metastatic, NM, at the cellular size level is important for early diagnosis and treatment. We present timely warning markers of metastasis, not identified by existing protocols and used methods. Digitized atomic force microscopy images of human histological cross-sections of M and NM colorectal cancer cells were analyzed by multifractal detrended fluctuation analysis and the generalized moments method analysis. Findings emphasize the multifractal character of all samples and accentuate room for the differentiation of M from NM cross-sections. Two universal markers emphatically achieve this goal performing very well: (a) the ratio of the singularity parameters (left/right), which are defined relative to weak/strong fluctuations in the multifractal spectrum, is always greater than 0.8 for NM tissues; and (b) the index of multifractality, used to classify universal multifractals, points to log-normal distribution for NM and to log-Cauchy for M tissues. An immediate large-scale screening of cancerous sections is doable based on these findings.https://www.mdpi.com/2072-6694/14/15/3728atomic force microscopy imagingmetastatic cancermultifractal analysis |
| spellingShingle | Evangelos Bakalis Angelo Ferraro Vassilios Gavriil Francesco Pepe Zoe Kollia Alkiviadis-Constantinos Cefalas Umberto Malapelle Evangelia Sarantopoulou Giancarlo Troncone Francesco Zerbetto Universal Markers Unveil Metastatic Cancerous Cross-Sections at Nanoscale Cancers atomic force microscopy imaging metastatic cancer multifractal analysis |
| title | Universal Markers Unveil Metastatic Cancerous Cross-Sections at Nanoscale |
| title_full | Universal Markers Unveil Metastatic Cancerous Cross-Sections at Nanoscale |
| title_fullStr | Universal Markers Unveil Metastatic Cancerous Cross-Sections at Nanoscale |
| title_full_unstemmed | Universal Markers Unveil Metastatic Cancerous Cross-Sections at Nanoscale |
| title_short | Universal Markers Unveil Metastatic Cancerous Cross-Sections at Nanoscale |
| title_sort | universal markers unveil metastatic cancerous cross sections at nanoscale |
| topic | atomic force microscopy imaging metastatic cancer multifractal analysis |
| url | https://www.mdpi.com/2072-6694/14/15/3728 |
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