Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish Embryos
Hypoxia induces precocious hatching in zebrafish, but we do not have a clear understanding of the molecular mechanisms regulating the activation of the hatching enzyme or how these mechanisms trigger precocious hatching under unfavorable environmental conditions. Using immunohistochemistry, pharmaco...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Journal of Developmental Biology |
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| Online Access: | https://www.mdpi.com/2221-3759/8/1/3 |
| _version_ | 1818270596944887808 |
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| author | Christopher D. Small Megan el-Khoury Ghislain Deslongchamps Tillmann J. Benfey Bryan D. Crawford |
| author_facet | Christopher D. Small Megan el-Khoury Ghislain Deslongchamps Tillmann J. Benfey Bryan D. Crawford |
| author_sort | Christopher D. Small |
| collection | DOAJ |
| description | Hypoxia induces precocious hatching in zebrafish, but we do not have a clear understanding of the molecular mechanisms regulating the activation of the hatching enzyme or how these mechanisms trigger precocious hatching under unfavorable environmental conditions. Using immunohistochemistry, pharmacological inhibition of matrix metalloproteinase 13 (Mmp13), and in vivo zymography, we show that Mmp13a is present in the hatching gland just as embryos become hatching competent and that Mmp13a activity is<b> </b>required for both normal hatching and hypoxia-induced precocious hatching. We conclude that Mmp13a likely functions in activating the hatching enzyme zymogen and that Mmp13a activity is necessary but not sufficient for hatching in zebrafish. This study highlights the broad nature of MMP function in development and provides a non-mammalian example of extra-embryonic processes mediated by MMP activity. |
| first_indexed | 2024-12-12T21:12:48Z |
| format | Article |
| id | doaj.art-188f5085330840d2a51642868007952f |
| institution | Directory Open Access Journal |
| issn | 2221-3759 |
| language | English |
| last_indexed | 2024-12-12T21:12:48Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Developmental Biology |
| spelling | doaj.art-188f5085330840d2a51642868007952f2022-12-22T00:11:50ZengMDPI AGJournal of Developmental Biology2221-37592020-01-0181310.3390/jdb8010003jdb8010003Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish EmbryosChristopher D. Small0Megan el-Khoury1Ghislain Deslongchamps2Tillmann J. Benfey3Bryan D. Crawford4Biology Department, University of New Brunswick, Fredericton, NB E3B 5A3, CanadaBiology Department, University of New Brunswick, Fredericton, NB E3B 5A3, CanadaChemistry Department, University of New Brunswick, Fredericton, NB E3B 5A3, CanadaBiology Department, University of New Brunswick, Fredericton, NB E3B 5A3, CanadaBiology Department, University of New Brunswick, Fredericton, NB E3B 5A3, CanadaHypoxia induces precocious hatching in zebrafish, but we do not have a clear understanding of the molecular mechanisms regulating the activation of the hatching enzyme or how these mechanisms trigger precocious hatching under unfavorable environmental conditions. Using immunohistochemistry, pharmacological inhibition of matrix metalloproteinase 13 (Mmp13), and in vivo zymography, we show that Mmp13a is present in the hatching gland just as embryos become hatching competent and that Mmp13a activity is<b> </b>required for both normal hatching and hypoxia-induced precocious hatching. We conclude that Mmp13a likely functions in activating the hatching enzyme zymogen and that Mmp13a activity is necessary but not sufficient for hatching in zebrafish. This study highlights the broad nature of MMP function in development and provides a non-mammalian example of extra-embryonic processes mediated by MMP activity.https://www.mdpi.com/2221-3759/8/1/3hatchingmatrix metalloproteinase 13 (mmp13)zebrafishhypoxiain vivo zymographystressdevelopmental trade-off |
| spellingShingle | Christopher D. Small Megan el-Khoury Ghislain Deslongchamps Tillmann J. Benfey Bryan D. Crawford Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish Embryos Journal of Developmental Biology hatching matrix metalloproteinase 13 (mmp13) zebrafish hypoxia in vivo zymography stress developmental trade-off |
| title | Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish Embryos |
| title_full | Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish Embryos |
| title_fullStr | Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish Embryos |
| title_full_unstemmed | Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish Embryos |
| title_short | Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish Embryos |
| title_sort | matrix metalloproteinase 13 activity is required for normal and hypoxia induced precocious hatching in zebrafish embryos |
| topic | hatching matrix metalloproteinase 13 (mmp13) zebrafish hypoxia in vivo zymography stress developmental trade-off |
| url | https://www.mdpi.com/2221-3759/8/1/3 |
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