Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats
Objective This study evaluates goat sperm motility in response to metabolic substrates and various inhibitors, aiming to assess the relative contribution of glycolysis and mitochondrial oxidation for sperm movement and adenosine triphosphate (ATP) production. Methods In the present study, two main m...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2024-04-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-23-0229.pdf |
| _version_ | 1797208765837082624 |
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| author | Rangga Setiawan Raden Febrianto Christi Ken Ratu Gharizah Alhuur Rini Widyastuti Nurcholidah Solihati Siti Darodjah Rasad Kundrat Hidajat Duy Ngoc Do |
| author_facet | Rangga Setiawan Raden Febrianto Christi Ken Ratu Gharizah Alhuur Rini Widyastuti Nurcholidah Solihati Siti Darodjah Rasad Kundrat Hidajat Duy Ngoc Do |
| author_sort | Rangga Setiawan |
| collection | DOAJ |
| description | Objective This study evaluates goat sperm motility in response to metabolic substrates and various inhibitors, aiming to assess the relative contribution of glycolysis and mitochondrial oxidation for sperm movement and adenosine triphosphate (ATP) production. Methods In the present study, two main metabolic substrates; 0 to 0.5 mM glucose and 0 to 30 mM pyruvate were used to evaluate their contribution to sperm movements of goats. Using a 3-chloro-1,2-propanediol (3-MCPD), a specific inhibitor for glycolysis, and carbonyl cyanide 3-chlorophenylhydrazone as an inhibitor for oxidative phosphorylation, cellular mechanisms into ATP-generating pathways in relation to sperm movements and ATP production were observed. Data were analysed using one-way analysis of variance for multiple comparisons. Results Sperm motility analysis showed that either glucose or pyruvate supported sperm movement during 0 to 30 min incubation. However, the supporting effects were abolished by the addition of a glycolysis inhibitor or mitochondrial uncoupler, concomitant with a significant decrease in ATP production. Although oxidative phosphorylation produces larger ATP concentrations than those from glycolysis, sperm progressivity in relation to these two metabolic pathways is comparable. Conclusion Based on the present study, we suggest that goat sperm use glucose and pyruvate to generate cellular energy through glycolysis and mitochondrial respiration pathways to maintain sperm movement. |
| first_indexed | 2024-03-07T14:29:44Z |
| format | Article |
| id | doaj.art-18920106130d4ac7b3d8a52ca5b37c03 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-24T09:44:01Z |
| publishDate | 2024-04-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-18920106130d4ac7b3d8a52ca5b37c032024-04-15T04:05:41ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352024-04-0137463163910.5713/ab.23.022925130Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goatsRangga Setiawan0Raden Febrianto Christi1Ken Ratu Gharizah Alhuur2Rini Widyastuti3Nurcholidah Solihati4Siti Darodjah Rasad5Kundrat Hidajat6Duy Ngoc Do7 Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung-Sumedang KM 21, West Java 45363, Indonesia Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung-Sumedang KM 21, West Java 45363, Indonesia Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung-Sumedang KM 21, West Java 45363, Indonesia Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung-Sumedang KM 21, West Java 45363, Indonesia Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung-Sumedang KM 21, West Java 45363, Indonesia Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung-Sumedang KM 21, West Java 45363, Indonesia Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung-Sumedang KM 21, West Java 45363, Indonesia Department of Animal Science and Aquaculture, Dalhousie University, Truro, NS B2N 5E3, CanadaObjective This study evaluates goat sperm motility in response to metabolic substrates and various inhibitors, aiming to assess the relative contribution of glycolysis and mitochondrial oxidation for sperm movement and adenosine triphosphate (ATP) production. Methods In the present study, two main metabolic substrates; 0 to 0.5 mM glucose and 0 to 30 mM pyruvate were used to evaluate their contribution to sperm movements of goats. Using a 3-chloro-1,2-propanediol (3-MCPD), a specific inhibitor for glycolysis, and carbonyl cyanide 3-chlorophenylhydrazone as an inhibitor for oxidative phosphorylation, cellular mechanisms into ATP-generating pathways in relation to sperm movements and ATP production were observed. Data were analysed using one-way analysis of variance for multiple comparisons. Results Sperm motility analysis showed that either glucose or pyruvate supported sperm movement during 0 to 30 min incubation. However, the supporting effects were abolished by the addition of a glycolysis inhibitor or mitochondrial uncoupler, concomitant with a significant decrease in ATP production. Although oxidative phosphorylation produces larger ATP concentrations than those from glycolysis, sperm progressivity in relation to these two metabolic pathways is comparable. Conclusion Based on the present study, we suggest that goat sperm use glucose and pyruvate to generate cellular energy through glycolysis and mitochondrial respiration pathways to maintain sperm movement.http://www.animbiosci.org/upload/pdf/ab-23-0229.pdfadenosine triphosphateglycolysisgoatoxidative phosphorylationsperm |
| spellingShingle | Rangga Setiawan Raden Febrianto Christi Ken Ratu Gharizah Alhuur Rini Widyastuti Nurcholidah Solihati Siti Darodjah Rasad Kundrat Hidajat Duy Ngoc Do Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats Animal Bioscience adenosine triphosphate glycolysis goat oxidative phosphorylation sperm |
| title | Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats |
| title_full | Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats |
| title_fullStr | Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats |
| title_full_unstemmed | Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats |
| title_short | Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats |
| title_sort | impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats |
| topic | adenosine triphosphate glycolysis goat oxidative phosphorylation sperm |
| url | http://www.animbiosci.org/upload/pdf/ab-23-0229.pdf |
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