A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids
Matrix-assisted laser desorption and ionization (MALDI) is a widely used soft-ionization technique of modern mass spectrometry (MS). MALDI enables the analysis of nearly all chemical compounds—including polar and apolar (phospho)lipids—with a minimum extent of fragmentation. MALDI has some particula...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/3/546 |
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| author | Jenny Leopold Patricia Prabutzki Kathrin M. Engel Jürgen Schiller |
| author_facet | Jenny Leopold Patricia Prabutzki Kathrin M. Engel Jürgen Schiller |
| author_sort | Jenny Leopold |
| collection | DOAJ |
| description | Matrix-assisted laser desorption and ionization (MALDI) is a widely used soft-ionization technique of modern mass spectrometry (MS). MALDI enables the analysis of nearly all chemical compounds—including polar and apolar (phospho)lipids—with a minimum extent of fragmentation. MALDI has some particular advantages (such as the possibility to acquire spatially-resolved spectra) and is competitive with the simultaneously developed ESI (electrospray ionization) MS. Although there are still some methodological aspects that need to be elucidated in more detail, it is obvious that the careful selection of an appropriate matrix plays the most important role in (lipid) analysis. Some lipid classes can be detected exclusively if the optimum matrix is used, and the matrix determines the sensitivity by which a particular lipid is detected within a mixture. Since the matrix is, thus, crucial for optimum results, we provide here an update on the progress in the field since our original review in this journal in 2018. Thus, only the development during the last five years is considered, and lipids are sorted according to increasing complexity, starting with free fatty acids and ending with cardiolipins and phosphoinositides. |
| first_indexed | 2024-03-11T06:52:14Z |
| format | Article |
| id | doaj.art-1897d6b3c231412eabfbf0474d740aba |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T06:52:14Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-1897d6b3c231412eabfbf0474d740aba2023-11-17T09:52:49ZengMDPI AGBiomolecules2218-273X2023-03-0113354610.3390/biom13030546A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of LipidsJenny Leopold0Patricia Prabutzki1Kathrin M. Engel2Jürgen Schiller3Faculty of Medicine, Institute for Medical Physics and Biophysics, Leipzig University, Härtelstr. 16/18, D-04107 Leipzig, GermanyFaculty of Medicine, Institute for Medical Physics and Biophysics, Leipzig University, Härtelstr. 16/18, D-04107 Leipzig, GermanyFaculty of Medicine, Institute for Medical Physics and Biophysics, Leipzig University, Härtelstr. 16/18, D-04107 Leipzig, GermanyFaculty of Medicine, Institute for Medical Physics and Biophysics, Leipzig University, Härtelstr. 16/18, D-04107 Leipzig, GermanyMatrix-assisted laser desorption and ionization (MALDI) is a widely used soft-ionization technique of modern mass spectrometry (MS). MALDI enables the analysis of nearly all chemical compounds—including polar and apolar (phospho)lipids—with a minimum extent of fragmentation. MALDI has some particular advantages (such as the possibility to acquire spatially-resolved spectra) and is competitive with the simultaneously developed ESI (electrospray ionization) MS. Although there are still some methodological aspects that need to be elucidated in more detail, it is obvious that the careful selection of an appropriate matrix plays the most important role in (lipid) analysis. Some lipid classes can be detected exclusively if the optimum matrix is used, and the matrix determines the sensitivity by which a particular lipid is detected within a mixture. Since the matrix is, thus, crucial for optimum results, we provide here an update on the progress in the field since our original review in this journal in 2018. Thus, only the development during the last five years is considered, and lipids are sorted according to increasing complexity, starting with free fatty acids and ending with cardiolipins and phosphoinositides.https://www.mdpi.com/2218-273X/13/3/546MALDI-MSmatrixlipidphospholipid |
| spellingShingle | Jenny Leopold Patricia Prabutzki Kathrin M. Engel Jürgen Schiller A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids Biomolecules MALDI-MS matrix lipid phospholipid |
| title | A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids |
| title_full | A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids |
| title_fullStr | A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids |
| title_full_unstemmed | A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids |
| title_short | A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids |
| title_sort | five year update on matrix compounds for maldi ms analysis of lipids |
| topic | MALDI-MS matrix lipid phospholipid |
| url | https://www.mdpi.com/2218-273X/13/3/546 |
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