Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients
Human prion diseases are neurodegenerative disorders caused by abnormally folded prion proteins in the central nervous system. These proteins can be detected using the quaking-induced conversion assay. Compared with other bioassays, this assay is extremely sensitive and was used in the present study...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | EBioMedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396416303887 |
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| author | Hanae Takatsuki, PhD Takayuki Fuse, PhD Takehiro Nakagaki, MD, PhD Tsuyoshi Mori, PhD Ban Mihara, MD, PhD Masaki Takao, MD, PhD Yasushi Iwasaki, MD, PhD Mari Yoshida, MD, PhD Shigeo Murayama, MD, PhD Ryuichiro Atarashi, MD, PhD Noriyuki Nishida, MD, PhD Katsuya Satoh, MD, PhD |
| author_facet | Hanae Takatsuki, PhD Takayuki Fuse, PhD Takehiro Nakagaki, MD, PhD Tsuyoshi Mori, PhD Ban Mihara, MD, PhD Masaki Takao, MD, PhD Yasushi Iwasaki, MD, PhD Mari Yoshida, MD, PhD Shigeo Murayama, MD, PhD Ryuichiro Atarashi, MD, PhD Noriyuki Nishida, MD, PhD Katsuya Satoh, MD, PhD |
| author_sort | Hanae Takatsuki, PhD |
| collection | DOAJ |
| description | Human prion diseases are neurodegenerative disorders caused by abnormally folded prion proteins in the central nervous system. These proteins can be detected using the quaking-induced conversion assay. Compared with other bioassays, this assay is extremely sensitive and was used in the present study to determine prion distribution in sporadic Creutzfeldt-Jakob disease patients at autopsy. Although infectivity of the sporadic form is thought to be restricted within the central nervous system, results showed that prion-seeding activities reach 106/g from a 50% seeding dose in non-neuronal tissues, suggesting that prion-seeding activity exists in non-neural organs, and we suggested that non-neural tissues of 106/g SD50 did not exist the infectivity. |
| first_indexed | 2024-12-17T08:38:34Z |
| format | Article |
| id | doaj.art-18982ab983274cdc80ccd6ad61d4e4e7 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-17T08:38:34Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-18982ab983274cdc80ccd6ad61d4e4e72022-12-21T21:56:24ZengElsevierEBioMedicine2352-39642016-10-0112C15015510.1016/j.ebiom.2016.08.033Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease PatientsHanae Takatsuki, PhD0Takayuki Fuse, PhD1Takehiro Nakagaki, MD, PhD2Tsuyoshi Mori, PhD3Ban Mihara, MD, PhD4Masaki Takao, MD, PhD5Yasushi Iwasaki, MD, PhD6Mari Yoshida, MD, PhD7Shigeo Murayama, MD, PhD8Ryuichiro Atarashi, MD, PhD9Noriyuki Nishida, MD, PhD10Katsuya Satoh, MD, PhD11Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, JapanDepartment of Neurology, Institute of Brain and Blood Vessels, Mihara Memorial Hospital, Isesaki, JapanDepartment of Neurology, Institute of Brain and Blood Vessels, Mihara Memorial Hospital, Isesaki, JapanDepartment of Neuropathology, Institute for Medical Science of Aging, Aichi Medical University, Aichi, JapanDepartment of Neuropathology, Institute for Medical Science of Aging, Aichi Medical University, Aichi, JapanDepartment of Neuropathology, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, Tokyo, JapanDepartment of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, JapanDepartment of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanHuman prion diseases are neurodegenerative disorders caused by abnormally folded prion proteins in the central nervous system. These proteins can be detected using the quaking-induced conversion assay. Compared with other bioassays, this assay is extremely sensitive and was used in the present study to determine prion distribution in sporadic Creutzfeldt-Jakob disease patients at autopsy. Although infectivity of the sporadic form is thought to be restricted within the central nervous system, results showed that prion-seeding activities reach 106/g from a 50% seeding dose in non-neuronal tissues, suggesting that prion-seeding activity exists in non-neural organs, and we suggested that non-neural tissues of 106/g SD50 did not exist the infectivity.http://www.sciencedirect.com/science/article/pii/S2352396416303887PrionPrion-seeding activitySD50Non-neural tissueCreutzfeldt-Jakob disease |
| spellingShingle | Hanae Takatsuki, PhD Takayuki Fuse, PhD Takehiro Nakagaki, MD, PhD Tsuyoshi Mori, PhD Ban Mihara, MD, PhD Masaki Takao, MD, PhD Yasushi Iwasaki, MD, PhD Mari Yoshida, MD, PhD Shigeo Murayama, MD, PhD Ryuichiro Atarashi, MD, PhD Noriyuki Nishida, MD, PhD Katsuya Satoh, MD, PhD Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients EBioMedicine Prion Prion-seeding activity SD50 Non-neural tissue Creutzfeldt-Jakob disease |
| title | Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients |
| title_full | Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients |
| title_fullStr | Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients |
| title_full_unstemmed | Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients |
| title_short | Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients |
| title_sort | prion seeding activity is widely distributed in tissues of sporadic creutzfeldt jakob disease patients |
| topic | Prion Prion-seeding activity SD50 Non-neural tissue Creutzfeldt-Jakob disease |
| url | http://www.sciencedirect.com/science/article/pii/S2352396416303887 |
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