Vitamin supplements enhance Spirulina platensis biomass and phytochemical contents
Spirulina platensis (SP) has a high impact on multidiscipline usage worldwide. Vitamins are considered as growth promoters due to their metabolic bio-regulating roles. This study was conducted to investigate the effect of vitamins: riboflavin (B2), thiamine (B1), and ascorbic acid (C) on SP growth a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-03-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2022-0028 |
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| author | Hamouda Ragaa A. El-Boraey Neveen G. El Bialy Badr E. Alrdahe Salma Saleh Darwish Doaa Bahaa Eldin |
| author_facet | Hamouda Ragaa A. El-Boraey Neveen G. El Bialy Badr E. Alrdahe Salma Saleh Darwish Doaa Bahaa Eldin |
| author_sort | Hamouda Ragaa A. |
| collection | DOAJ |
| description | Spirulina platensis (SP) has a high impact on multidiscipline usage worldwide. Vitamins are considered as growth promoters due to their metabolic bio-regulating roles. This study was conducted to investigate the effect of vitamins: riboflavin (B2), thiamine (B1), and ascorbic acid (C) on SP growth and contents of pigments, phenols, and phytochemicals besides its antioxidant activities. Vitamins were added in different concentrations to Zarrouk’s medium and tested for their effects weekly for three weeks of spirulina cultivation. The results revealed that 25 mg·L−1 thiamine or riboflavin promoted the most significant contents of chlorophyll and carotenoids, respectively, after 14 days of cultivation. However, adding 10 mg·L−1 thiamine achieved the most significant increase in algal biomass yield and contents of chlorophyll, carotenoids phycocyanin, allophycocyanin, phycoerythrin, and phycobiliprotein after 21 days of cultivation. Qualitative analysis showed that both SP and SP supplemented with 10 mg·L−1 thiamine (SPt) for 21 days contain tannins and flavonoids but quantitative analysis approved that SPt recorded significant increase in phenolic and tannin contents. Moreover, SPt induced a significant increase of total antioxidant activity in vitro 1,1-diphenyl-1,2-picrylhydrazyl assay in comparison with SP. Vitamins especially thiamine added during SP culture could improve SP biomasses, pigments, and phytochemical contents and hence increased antioxidant capacity. |
| first_indexed | 2024-04-12T03:00:19Z |
| format | Article |
| id | doaj.art-189cc9e5f3b1438a812404b8c34bdce9 |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-04-12T03:00:19Z |
| publishDate | 2022-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-189cc9e5f3b1438a812404b8c34bdce92022-12-22T03:50:41ZengDe GruyterGreen Processing and Synthesis2191-95502022-03-0111126627410.1515/gps-2022-0028Vitamin supplements enhance Spirulina platensis biomass and phytochemical contentsHamouda Ragaa A.0El-Boraey Neveen G.1El Bialy Badr E.2Alrdahe Salma Saleh3Darwish Doaa Bahaa Eldin4Department of Biology, Faculty of Sciences and Arts Khulais, University of Jeddah, Jeddah, Saudi ArabiaDepartment of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, 32897, EgyptDepartment of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, EgyptDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi ArabiaDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi ArabiaSpirulina platensis (SP) has a high impact on multidiscipline usage worldwide. Vitamins are considered as growth promoters due to their metabolic bio-regulating roles. This study was conducted to investigate the effect of vitamins: riboflavin (B2), thiamine (B1), and ascorbic acid (C) on SP growth and contents of pigments, phenols, and phytochemicals besides its antioxidant activities. Vitamins were added in different concentrations to Zarrouk’s medium and tested for their effects weekly for three weeks of spirulina cultivation. The results revealed that 25 mg·L−1 thiamine or riboflavin promoted the most significant contents of chlorophyll and carotenoids, respectively, after 14 days of cultivation. However, adding 10 mg·L−1 thiamine achieved the most significant increase in algal biomass yield and contents of chlorophyll, carotenoids phycocyanin, allophycocyanin, phycoerythrin, and phycobiliprotein after 21 days of cultivation. Qualitative analysis showed that both SP and SP supplemented with 10 mg·L−1 thiamine (SPt) for 21 days contain tannins and flavonoids but quantitative analysis approved that SPt recorded significant increase in phenolic and tannin contents. Moreover, SPt induced a significant increase of total antioxidant activity in vitro 1,1-diphenyl-1,2-picrylhydrazyl assay in comparison with SP. Vitamins especially thiamine added during SP culture could improve SP biomasses, pigments, and phytochemical contents and hence increased antioxidant capacity.https://doi.org/10.1515/gps-2022-0028spirulina platensisvitaminsbiomassphytochemicalsantioxidants |
| spellingShingle | Hamouda Ragaa A. El-Boraey Neveen G. El Bialy Badr E. Alrdahe Salma Saleh Darwish Doaa Bahaa Eldin Vitamin supplements enhance Spirulina platensis biomass and phytochemical contents Green Processing and Synthesis spirulina platensis vitamins biomass phytochemicals antioxidants |
| title | Vitamin supplements enhance Spirulina platensis biomass and phytochemical contents |
| title_full | Vitamin supplements enhance Spirulina platensis biomass and phytochemical contents |
| title_fullStr | Vitamin supplements enhance Spirulina platensis biomass and phytochemical contents |
| title_full_unstemmed | Vitamin supplements enhance Spirulina platensis biomass and phytochemical contents |
| title_short | Vitamin supplements enhance Spirulina platensis biomass and phytochemical contents |
| title_sort | vitamin supplements enhance spirulina platensis biomass and phytochemical contents |
| topic | spirulina platensis vitamins biomass phytochemicals antioxidants |
| url | https://doi.org/10.1515/gps-2022-0028 |
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