Molecular characterization of vibriosis associated bacteria from traditional mud-crab farmed in the North Coast of Central Java, Indonesia
This study examines the molecular characterization of bacteria associated with clinical symptoms and the diversity of vibriosis infecting farmed mud crab from three sampling locations, namely Rembang, Demak, and Kendal Districts. The clinical symptom was red-brown spots on carapace and wounds. Twe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2022-08-01
|
| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://sjst.psu.ac.th/journal/44-4/6.pdf |
| _version_ | 1797844308756267008 |
|---|---|
| author | Sarjito Sarjito Alfabetian Harjuno Condro Haditomo Slamet Budi Prayitno Aninditia Sabdaningsih Desrina Restiana Wisnu Ariyati |
| author_facet | Sarjito Sarjito Alfabetian Harjuno Condro Haditomo Slamet Budi Prayitno Aninditia Sabdaningsih Desrina Restiana Wisnu Ariyati |
| author_sort | Sarjito Sarjito |
| collection | DOAJ |
| description | This study examines the molecular characterization of bacteria associated with clinical symptoms and the diversity of
vibriosis infecting farmed mud crab from three sampling locations, namely Rembang, Demak, and Kendal Districts. The clinical
symptom was red-brown spots on carapace and wounds. Twenty-five bacterial isolates were gained from hepatopancreas, gills
and carapace of nine infected mud crabs, by culturing in TCBS and TSA medium. Molecular characterization was carried out
through modified rep-PCR followed by 16S-rRNA gene amplification. The results indicate that seven out of the 25 isolates,
namely CJR14, CJR15, CJD23, CJK10, CJK11, CJD22 and CJR05 were 92-98% firmly related to Vibrio harveyi NCIMB1280, Catenococcus thiocicly TG5-3, Photobacterium ganghwense FR311, Vibrio parahaemolyticus ATCC-1780, Shewanella
loihica PV4, Shewanella algae ATCC5, and Vibrio alginolyticus NBRC-15630. This study revealed that the 25 isolates found
from infected mud crabs fell into seven groups of bacteria. These seven groups were well-known as pathogens for aquatic
organisms. Moreover, according to molecular characterization, the highest diversity of Vibrionaceae was obtained from
Rembang, and the bacterium most found in all three sampling sites was C. thiocicly. |
| first_indexed | 2024-04-09T17:20:13Z |
| format | Article |
| id | doaj.art-189da0eb39ba4920a82bc3a402549385 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-09T17:20:13Z |
| publishDate | 2022-08-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-189da0eb39ba4920a82bc3a4025493852023-04-19T03:57:17ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952022-08-0144494595210.14456/sjst-psu.2022.126Molecular characterization of vibriosis associated bacteria from traditional mud-crab farmed in the North Coast of Central Java, IndonesiaSarjito Sarjito0Alfabetian Harjuno Condro Haditomo1Slamet Budi Prayitno2Aninditia Sabdaningsih3Desrina4Restiana Wisnu Ariyati5Aquaculture Study Program, Department of Aquaculture, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50275 IndonesiaAquaculture Study Program, Department of Aquaculture, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50275 IndonesiaAquaculture Study Program, Department of Aquaculture, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50275 IndonesiaDepartment of Aquatic Resources, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50275 IndonesiaAquaculture Study Program, Department of Aquaculture, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50275 IndonesiaAquaculture Study Program, Department of Aquaculture, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50275 IndonesiaThis study examines the molecular characterization of bacteria associated with clinical symptoms and the diversity of vibriosis infecting farmed mud crab from three sampling locations, namely Rembang, Demak, and Kendal Districts. The clinical symptom was red-brown spots on carapace and wounds. Twenty-five bacterial isolates were gained from hepatopancreas, gills and carapace of nine infected mud crabs, by culturing in TCBS and TSA medium. Molecular characterization was carried out through modified rep-PCR followed by 16S-rRNA gene amplification. The results indicate that seven out of the 25 isolates, namely CJR14, CJR15, CJD23, CJK10, CJK11, CJD22 and CJR05 were 92-98% firmly related to Vibrio harveyi NCIMB1280, Catenococcus thiocicly TG5-3, Photobacterium ganghwense FR311, Vibrio parahaemolyticus ATCC-1780, Shewanella loihica PV4, Shewanella algae ATCC5, and Vibrio alginolyticus NBRC-15630. This study revealed that the 25 isolates found from infected mud crabs fell into seven groups of bacteria. These seven groups were well-known as pathogens for aquatic organisms. Moreover, according to molecular characterization, the highest diversity of Vibrionaceae was obtained from Rembang, and the bacterium most found in all three sampling sites was C. thiocicly.https://sjst.psu.ac.th/journal/44-4/6.pdfmud crab16s rrnarep pcrvibriosis |
| spellingShingle | Sarjito Sarjito Alfabetian Harjuno Condro Haditomo Slamet Budi Prayitno Aninditia Sabdaningsih Desrina Restiana Wisnu Ariyati Molecular characterization of vibriosis associated bacteria from traditional mud-crab farmed in the North Coast of Central Java, Indonesia Songklanakarin Journal of Science and Technology (SJST) mud crab 16s rrna rep pcr vibriosis |
| title | Molecular characterization of vibriosis associated bacteria from traditional mud-crab farmed in the North Coast of Central Java, Indonesia |
| title_full | Molecular characterization of vibriosis associated bacteria from traditional mud-crab farmed in the North Coast of Central Java, Indonesia |
| title_fullStr | Molecular characterization of vibriosis associated bacteria from traditional mud-crab farmed in the North Coast of Central Java, Indonesia |
| title_full_unstemmed | Molecular characterization of vibriosis associated bacteria from traditional mud-crab farmed in the North Coast of Central Java, Indonesia |
| title_short | Molecular characterization of vibriosis associated bacteria from traditional mud-crab farmed in the North Coast of Central Java, Indonesia |
| title_sort | molecular characterization of vibriosis associated bacteria from traditional mud crab farmed in the north coast of central java indonesia |
| topic | mud crab 16s rrna rep pcr vibriosis |
| url | https://sjst.psu.ac.th/journal/44-4/6.pdf |
| work_keys_str_mv | AT sarjitosarjito molecularcharacterizationofvibriosisassociatedbacteriafromtraditionalmudcrabfarmedinthenorthcoastofcentraljavaindonesia AT alfabetianharjunocondrohaditomo molecularcharacterizationofvibriosisassociatedbacteriafromtraditionalmudcrabfarmedinthenorthcoastofcentraljavaindonesia AT slametbudiprayitno molecularcharacterizationofvibriosisassociatedbacteriafromtraditionalmudcrabfarmedinthenorthcoastofcentraljavaindonesia AT aninditiasabdaningsih molecularcharacterizationofvibriosisassociatedbacteriafromtraditionalmudcrabfarmedinthenorthcoastofcentraljavaindonesia AT desrina molecularcharacterizationofvibriosisassociatedbacteriafromtraditionalmudcrabfarmedinthenorthcoastofcentraljavaindonesia AT restianawisnuariyati molecularcharacterizationofvibriosisassociatedbacteriafromtraditionalmudcrabfarmedinthenorthcoastofcentraljavaindonesia |