An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker
Abstract Background Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | BMC Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12885-018-4006-5 |
| _version_ | 1818142957769850880 |
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| author | Takuma Goto Mikihiro Fujiya Hiroaki Konishi Junpei Sasajima Shugo Fujibayashi Akihiro Hayashi Tatsuya Utsumi Hiroki Sato Takuya Iwama Masami Ijiri Aki Sakatani Kazuyuki Tanaka Yoshiki Nomura Nobuhiro Ueno Shin Kashima Kentaro Moriichi Yusuke Mizukami Yutaka Kohgo Toshikatsu Okumura |
| author_facet | Takuma Goto Mikihiro Fujiya Hiroaki Konishi Junpei Sasajima Shugo Fujibayashi Akihiro Hayashi Tatsuya Utsumi Hiroki Sato Takuya Iwama Masami Ijiri Aki Sakatani Kazuyuki Tanaka Yoshiki Nomura Nobuhiro Ueno Shin Kashima Kentaro Moriichi Yusuke Mizukami Yutaka Kohgo Toshikatsu Okumura |
| author_sort | Takuma Goto |
| collection | DOAJ |
| description | Abstract Background Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers. Methods The ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™. The expression of ExmiRs was evaluated using a next-generation sequencing analysis, and the selected three miRs through this analysis were confirmed by a quantitative real-time polymerase chain reaction. Results The expression of ExmiR-191, ExmiR-21 and ExmiR-451a was significantly up-regulated in patients with pancreatic cancer and IPMN compared to the controls (p < 0.05). A receiver operating characteristic curve analysis showed that the area under the curve and the diagnostic accuracy of ExmiRs were 5–20% superior to those of three serum bulky circulating miRs (e.g.; ExmiR-21: AUC 0.826, accuracy 80.8%. Circulating miR-21: AUC 0.653, accuracy 62.3%). In addition, high ExmiR-451a was associated with mural nodules in IPMN (p = 0.010), and high ExmiR-21 was identified as a candidate prognostic factor for the overall survival (p = 0.011, HR 4.071, median OS of high-ExmiR-21: 344 days, median OS of low-ExmiR-21: 846 days) and chemo-resistant markers (p = 0.022). Conclusions The level of three ExmiRs can thus serve as early diagnostic and progression markers of pancreatic cancer and IPMN, and considered more useful markers than the circulating miRs (limited to these three miRs). |
| first_indexed | 2024-12-11T11:24:02Z |
| format | Article |
| id | doaj.art-189e5a9629a94836a90a61b3e38d93d4 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T11:24:02Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-189e5a9629a94836a90a61b3e38d93d42022-12-22T01:09:03ZengBMCBMC Cancer1471-24072018-01-0118111110.1186/s12885-018-4006-5An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic markerTakuma Goto0Mikihiro Fujiya1Hiroaki Konishi2Junpei Sasajima3Shugo Fujibayashi4Akihiro Hayashi5Tatsuya Utsumi6Hiroki Sato7Takuya Iwama8Masami Ijiri9Aki Sakatani10Kazuyuki Tanaka11Yoshiki Nomura12Nobuhiro Ueno13Shin Kashima14Kentaro Moriichi15Yusuke Mizukami16Yutaka Kohgo17Toshikatsu Okumura18Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityDepartment of Gastroenterology, International University of Health and Welfare HospitalDivision of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical UniversityAbstract Background Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers. Methods The ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™. The expression of ExmiRs was evaluated using a next-generation sequencing analysis, and the selected three miRs through this analysis were confirmed by a quantitative real-time polymerase chain reaction. Results The expression of ExmiR-191, ExmiR-21 and ExmiR-451a was significantly up-regulated in patients with pancreatic cancer and IPMN compared to the controls (p < 0.05). A receiver operating characteristic curve analysis showed that the area under the curve and the diagnostic accuracy of ExmiRs were 5–20% superior to those of three serum bulky circulating miRs (e.g.; ExmiR-21: AUC 0.826, accuracy 80.8%. Circulating miR-21: AUC 0.653, accuracy 62.3%). In addition, high ExmiR-451a was associated with mural nodules in IPMN (p = 0.010), and high ExmiR-21 was identified as a candidate prognostic factor for the overall survival (p = 0.011, HR 4.071, median OS of high-ExmiR-21: 344 days, median OS of low-ExmiR-21: 846 days) and chemo-resistant markers (p = 0.022). Conclusions The level of three ExmiRs can thus serve as early diagnostic and progression markers of pancreatic cancer and IPMN, and considered more useful markers than the circulating miRs (limited to these three miRs).http://link.springer.com/article/10.1186/s12885-018-4006-5Pancreatic cancerExosomemicroRNA-21microRNA-451aIntraductal papillary mucinous neoplasmTumor marker |
| spellingShingle | Takuma Goto Mikihiro Fujiya Hiroaki Konishi Junpei Sasajima Shugo Fujibayashi Akihiro Hayashi Tatsuya Utsumi Hiroki Sato Takuya Iwama Masami Ijiri Aki Sakatani Kazuyuki Tanaka Yoshiki Nomura Nobuhiro Ueno Shin Kashima Kentaro Moriichi Yusuke Mizukami Yutaka Kohgo Toshikatsu Okumura An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker BMC Cancer Pancreatic cancer Exosome microRNA-21 microRNA-451a Intraductal papillary mucinous neoplasm Tumor marker |
| title | An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker |
| title_full | An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker |
| title_fullStr | An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker |
| title_full_unstemmed | An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker |
| title_short | An elevated expression of serum exosomal microRNA-191, − 21, −451a of pancreatic neoplasm is considered to be efficient diagnostic marker |
| title_sort | elevated expression of serum exosomal microrna 191 21 451a of pancreatic neoplasm is considered to be efficient diagnostic marker |
| topic | Pancreatic cancer Exosome microRNA-21 microRNA-451a Intraductal papillary mucinous neoplasm Tumor marker |
| url | http://link.springer.com/article/10.1186/s12885-018-4006-5 |
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