Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019
Shiga toxin–producing Escherichia coli (STEC) O80:H2 has emerged in Europe as a cause of hemolytic uremic syndrome associated with bacteremia. STEC O80:H2 harbors the mosaic plasmid pR444_A, which combines several virulence genes, including hlyF and antimicrobial resistance genes. pR444_A is found i...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2021-03-01
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| Series: | Emerging Infectious Diseases |
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| Online Access: | https://wwwnc.cdc.gov/eid/article/27/3/20-3110_article |
| _version_ | 1818865975353671680 |
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| author | Federica Gigliucci Angela H.A.M. van Hoek Paola Chiani Arnold Knijn Fabio Minelli Gaia Scavia Eelco Franz Stefano Morabito Valeria Michelacci |
| author_facet | Federica Gigliucci Angela H.A.M. van Hoek Paola Chiani Arnold Knijn Fabio Minelli Gaia Scavia Eelco Franz Stefano Morabito Valeria Michelacci |
| author_sort | Federica Gigliucci |
| collection | DOAJ |
| description | Shiga toxin–producing Escherichia coli (STEC) O80:H2 has emerged in Europe as a cause of hemolytic uremic syndrome associated with bacteremia. STEC O80:H2 harbors the mosaic plasmid pR444_A, which combines several virulence genes, including hlyF and antimicrobial resistance genes. pR444_A is found in some extraintestinal pathogenic E. coli (ExPEC) strains. We identified and characterized 53 STEC strains with ExPEC-associated virulence genes isolated in Italy and the Netherlands during 2000–2019. The isolates belong to 2 major populations: 1 belongs to sequence type 301 and harbors diverse stx2 subtypes, the intimin variant eae-ξ, and pO157-like and pR444_A plasmids; 1 consists of strains belonging to various sequence types, some of which lack the pO157 plasmid, the locus of enterocyte effacement, and the antimicrobial resistance–encoding region. Our results showed that STEC strains harboring ExPEC-associated virulence genes can include multiple serotypes and that the pR444_A plasmid can be acquired and mobilized by STEC strains. |
| first_indexed | 2024-12-19T10:56:05Z |
| format | Article |
| id | doaj.art-18a1f65b31f84e1196db7ac4b47c3367 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-19T10:56:05Z |
| publishDate | 2021-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-18a1f65b31f84e1196db7ac4b47c33672022-12-21T20:24:49ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592021-03-0127385386110.3201/eid2703.203110Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019Federica GigliucciAngela H.A.M. van HoekPaola ChianiArnold KnijnFabio MinelliGaia ScaviaEelco FranzStefano MorabitoValeria MichelacciShiga toxin–producing Escherichia coli (STEC) O80:H2 has emerged in Europe as a cause of hemolytic uremic syndrome associated with bacteremia. STEC O80:H2 harbors the mosaic plasmid pR444_A, which combines several virulence genes, including hlyF and antimicrobial resistance genes. pR444_A is found in some extraintestinal pathogenic E. coli (ExPEC) strains. We identified and characterized 53 STEC strains with ExPEC-associated virulence genes isolated in Italy and the Netherlands during 2000–2019. The isolates belong to 2 major populations: 1 belongs to sequence type 301 and harbors diverse stx2 subtypes, the intimin variant eae-ξ, and pO157-like and pR444_A plasmids; 1 consists of strains belonging to various sequence types, some of which lack the pO157 plasmid, the locus of enterocyte effacement, and the antimicrobial resistance–encoding region. Our results showed that STEC strains harboring ExPEC-associated virulence genes can include multiple serotypes and that the pR444_A plasmid can be acquired and mobilized by STEC strains.https://wwwnc.cdc.gov/eid/article/27/3/20-3110_articleShiga toxin–producing Escherichia coliextraintestinal pathogenic E. colihemolytic uremic syndromezoonosesfoodborne diseaseantimicrobial resistance |
| spellingShingle | Federica Gigliucci Angela H.A.M. van Hoek Paola Chiani Arnold Knijn Fabio Minelli Gaia Scavia Eelco Franz Stefano Morabito Valeria Michelacci Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019 Emerging Infectious Diseases Shiga toxin–producing Escherichia coli extraintestinal pathogenic E. coli hemolytic uremic syndrome zoonoses foodborne disease antimicrobial resistance |
| title | Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019 |
| title_full | Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019 |
| title_fullStr | Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019 |
| title_full_unstemmed | Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019 |
| title_short | Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019 |
| title_sort | genomic characterization of hlyf positive shiga toxin producing escherichia coli italy and the netherlands 2000 2019 |
| topic | Shiga toxin–producing Escherichia coli extraintestinal pathogenic E. coli hemolytic uremic syndrome zoonoses foodborne disease antimicrobial resistance |
| url | https://wwwnc.cdc.gov/eid/article/27/3/20-3110_article |
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