O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications
Aims: To analyze the clinical relevance of O6-methylguanine-DNA methyltransferase in rectal adenocarcinoma treated with chemoradiotherapy followed by surgery. Methods: Tissue samples from 29 rectal adenocarcinoma patients were obtained after chemoradiotherapy. O6-methylguanine-DNA methyltransferase...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2019-09-01
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| Series: | Balkan Medical Journal |
| Subjects: | |
| Online Access: | http://balkanmedicaljournal.org/text.php?lang=en&id=2100 |
| _version_ | 1797920183110598656 |
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| author | Jaime A. Oliver Jaime Gómez-Millán Jose A. Medina Laura Cabeza Gloria Perazzoli Cristina Jimenez-Luna Kevin Doello Raúl Ortiz |
| author_facet | Jaime A. Oliver Jaime Gómez-Millán Jose A. Medina Laura Cabeza Gloria Perazzoli Cristina Jimenez-Luna Kevin Doello Raúl Ortiz |
| author_sort | Jaime A. Oliver |
| collection | DOAJ |
| description | Aims: To analyze the clinical relevance of O6-methylguanine-DNA methyltransferase in rectal adenocarcinoma treated with chemoradiotherapy followed by surgery.
Methods: Tissue samples from 29 rectal adenocarcinoma patients were obtained after chemoradiotherapy. O6-methylguanine-DNA methyltransferase promoter methylation status was established by methylation-specific polymerase chain reaction. O6-methylguanine-DNA methyltransferase protein levels were determined by immunohistochemistry. Clinicopathologic variables, including treatment regression grade, recurrence, lymph node invasion, and stage and differentiation grade of the tumor, were determined.
Results: The O6-methylguanine-DNA methyltransferase gene promoter was methylated in 81.5% of samples. Most patients (88.9%) showed low O6-methylguanine-DNA methyltransferase protein expression. O6-methylguanine-DNA methyltransferase methylation status was not correlated with any of the clinicopathological variables determined in rectal adenocarcinomas selected for chemoradiotherapy.
Conclusion: O6-methylguanine-DNA methyltransferase methylation status is not correlated with clinicopathologic variables examined in rectal adenocarcinoma selected for chemoradiotherapy, although its role as a biomarker awaits further investigation. |
| first_indexed | 2024-04-10T13:57:15Z |
| format | Article |
| id | doaj.art-18a282223f4c45379944876be55e2d1f |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T13:57:15Z |
| publishDate | 2019-09-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-18a282223f4c45379944876be55e2d1f2023-02-15T16:10:25ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312019-09-0136528328610.4274/balkanmedj.galenos.2019.2018.12.93O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical ImplicationsJaime A. Oliver0Jaime Gómez-Millán1Jose A. Medina2Laura Cabeza3Gloria Perazzoli4Cristina Jimenez-Luna5Kevin Doello6Raúl Ortiz7Center for Cancer Research and Cell Biology, Queen’s University Belfast, Belfast, UKDepartment of Radiation Oncology, Universitary Hospital Virgen de la Victoria, Málaga, SpainDepartment of Radiation Oncology, Universitary Hospital Virgen de la Victoria, Málaga, SpainInstitute of Biopathology and Regenerative Medicine, Center of Biomedical Research, University of Granada, Granada, SpainInstitute of Biopathology and Regenerative Medicine, Center of Biomedical Research, University of Granada, Granada, SpainInstitute of Biopathology and Regenerative Medicine, Center of Biomedical Research, University of Granada, Granada, SpainMedical Oncology Service, Universitary Hospital Virgen de las Nieves, Granada, SpainBiosanitary Institute of Granada (ibs. GRANADA), SAS-Universidad de Granada, Granada, SpainAims: To analyze the clinical relevance of O6-methylguanine-DNA methyltransferase in rectal adenocarcinoma treated with chemoradiotherapy followed by surgery. Methods: Tissue samples from 29 rectal adenocarcinoma patients were obtained after chemoradiotherapy. O6-methylguanine-DNA methyltransferase promoter methylation status was established by methylation-specific polymerase chain reaction. O6-methylguanine-DNA methyltransferase protein levels were determined by immunohistochemistry. Clinicopathologic variables, including treatment regression grade, recurrence, lymph node invasion, and stage and differentiation grade of the tumor, were determined. Results: The O6-methylguanine-DNA methyltransferase gene promoter was methylated in 81.5% of samples. Most patients (88.9%) showed low O6-methylguanine-DNA methyltransferase protein expression. O6-methylguanine-DNA methyltransferase methylation status was not correlated with any of the clinicopathological variables determined in rectal adenocarcinomas selected for chemoradiotherapy. Conclusion: O6-methylguanine-DNA methyltransferase methylation status is not correlated with clinicopathologic variables examined in rectal adenocarcinoma selected for chemoradiotherapy, although its role as a biomarker awaits further investigation.http://balkanmedicaljournal.org/text.php?lang=en&id=2100ChemoradiotherapyO6-methylguanine-DNA methyltransferaserectal adenocarcinoma |
| spellingShingle | Jaime A. Oliver Jaime Gómez-Millán Jose A. Medina Laura Cabeza Gloria Perazzoli Cristina Jimenez-Luna Kevin Doello Raúl Ortiz O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications Balkan Medical Journal Chemoradiotherapy O6-methylguanine-DNA methyltransferase rectal adenocarcinoma |
| title | O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications |
| title_full | O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications |
| title_fullStr | O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications |
| title_full_unstemmed | O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications |
| title_short | O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications |
| title_sort | o6 methylguanine dna methyltransferase promoter methylation in patients with rectal adenocarcinoma after chemoradiotherapy treatment clinical implications |
| topic | Chemoradiotherapy O6-methylguanine-DNA methyltransferase rectal adenocarcinoma |
| url | http://balkanmedicaljournal.org/text.php?lang=en&id=2100 |
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