Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia
The use of synthetic medication for treating alopecia is restricted because of systemic exposure and related negative effects. Beta-sitosterol (β-ST), a natural chemical, has lately been studied for its potential to promote hair development. The cubosomes with dissolving microneedles (CUBs-MN<sub...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/4/563 |
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| author | Kousalya Prabahar Ubaidulla Uthumansha Nehal Elsherbiny Mona Qushawy |
| author_facet | Kousalya Prabahar Ubaidulla Uthumansha Nehal Elsherbiny Mona Qushawy |
| author_sort | Kousalya Prabahar |
| collection | DOAJ |
| description | The use of synthetic medication for treating alopecia is restricted because of systemic exposure and related negative effects. Beta-sitosterol (β-ST), a natural chemical, has lately been studied for its potential to promote hair development. The cubosomes with dissolving microneedles (CUBs-MN<sub>D</sub>) created in this study may be a useful starting point for the creation of a sophisticated dermal delivery system for β-ST. Cubosomes (CUBs) were prepared by the emulsification method, using glyceryl monooleate (GMO) as a lipid polymer. CUBs were loaded with dissolving microneedles (MN<sub>D</sub>) fabricated with HA and a PVP-K90 matrix. An ex vivo skin permeation study and an in vivo hair growth efficacy test of β-ST were performed with both CUB and CUB-MN<sub>D</sub>. The average particle size of the CUBs was determined to be 173.67 ± 0.52 nm, with a low polydispersity index (0.3) and a high zeta potential value that prevents the aggregate formation of dispersed particles. When compared to CUBs alone, CUBs-MN<sub>D</sub> displayed higher permeating levels of β-ST at all-time points. In the animals from the CUB-MN<sub>D</sub> group, significant hair development was observed. According to the results of the current investigation, CUBs that integrate dissolving microneedles of β-ST are superior in terms of transdermal skin penetration and activity for the treatment of alopecia. |
| first_indexed | 2024-03-11T04:39:00Z |
| format | Article |
| id | doaj.art-18a4ddd3fb644d7783b0072c70f78631 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T04:39:00Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | Pharmaceuticals |
| spelling | doaj.art-18a4ddd3fb644d7783b0072c70f786312023-11-17T20:50:42ZengMDPI AGPharmaceuticals1424-82472023-04-0116456310.3390/ph16040563Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of AlopeciaKousalya Prabahar0Ubaidulla Uthumansha1Nehal Elsherbiny2Mona Qushawy3Department of Pharmacy Practice, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi ArabiaDepartment of Pharmaceutics, Crescent School of Pharmacy, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai 600048, IndiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi ArabiaThe use of synthetic medication for treating alopecia is restricted because of systemic exposure and related negative effects. Beta-sitosterol (β-ST), a natural chemical, has lately been studied for its potential to promote hair development. The cubosomes with dissolving microneedles (CUBs-MN<sub>D</sub>) created in this study may be a useful starting point for the creation of a sophisticated dermal delivery system for β-ST. Cubosomes (CUBs) were prepared by the emulsification method, using glyceryl monooleate (GMO) as a lipid polymer. CUBs were loaded with dissolving microneedles (MN<sub>D</sub>) fabricated with HA and a PVP-K90 matrix. An ex vivo skin permeation study and an in vivo hair growth efficacy test of β-ST were performed with both CUB and CUB-MN<sub>D</sub>. The average particle size of the CUBs was determined to be 173.67 ± 0.52 nm, with a low polydispersity index (0.3) and a high zeta potential value that prevents the aggregate formation of dispersed particles. When compared to CUBs alone, CUBs-MN<sub>D</sub> displayed higher permeating levels of β-ST at all-time points. In the animals from the CUB-MN<sub>D</sub> group, significant hair development was observed. According to the results of the current investigation, CUBs that integrate dissolving microneedles of β-ST are superior in terms of transdermal skin penetration and activity for the treatment of alopecia.https://www.mdpi.com/1424-8247/16/4/563cubosomesmicroneedlesbeta-sitosterolalopeciadermal drug deliverytestosterone induced alopecia animal model |
| spellingShingle | Kousalya Prabahar Ubaidulla Uthumansha Nehal Elsherbiny Mona Qushawy Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia Pharmaceuticals cubosomes microneedles beta-sitosterol alopecia dermal drug delivery testosterone induced alopecia animal model |
| title | Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia |
| title_full | Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia |
| title_fullStr | Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia |
| title_full_unstemmed | Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia |
| title_short | Enhanced Skin Permeation and Controlled Release of β-Sitosterol Using Cubosomes Encrusted with Dissolving Microneedles for the Management of Alopecia |
| title_sort | enhanced skin permeation and controlled release of β sitosterol using cubosomes encrusted with dissolving microneedles for the management of alopecia |
| topic | cubosomes microneedles beta-sitosterol alopecia dermal drug delivery testosterone induced alopecia animal model |
| url | https://www.mdpi.com/1424-8247/16/4/563 |
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