Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing <i>Herniaria</i> L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro
The work is aimed at phytochemical characterization and In Vitro evaluation of antioxidant actions, anti-inflammatory effects, and cytotoxicity of purified extracts from three rupturewort (<i>Herniaria</i> L.) species, i.e., <i>Herniaria glabra</i> (HG), <i>H. polygama&...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/1420-3049/27/2/530 |
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| author | Solomiia Kozachok Joanna Kolodziejczyk-Czepas Svitlana Marchyshyn Krzysztof Kamil Wojtanowski Grażyna Zgórka Wieslaw Oleszek |
| author_facet | Solomiia Kozachok Joanna Kolodziejczyk-Czepas Svitlana Marchyshyn Krzysztof Kamil Wojtanowski Grażyna Zgórka Wieslaw Oleszek |
| author_sort | Solomiia Kozachok |
| collection | DOAJ |
| description | The work is aimed at phytochemical characterization and In Vitro evaluation of antioxidant actions, anti-inflammatory effects, and cytotoxicity of purified extracts from three rupturewort (<i>Herniaria</i> L.) species, i.e., <i>Herniaria glabra</i> (HG), <i>H. polygama</i> (HP), and <i>H. incana</i> herb (HIh). The total phenolic content established in the purified extracts (PEs) of HIh, HP, and HG was 29.6, 24.0, and 13.0%, respectively. Thirty-eight non-saponin metabolites were identified using LC-HR-QTOF-ESI-MS; however, only 9 were common for the studied <i>Herniaria</i> species. The most abundant phenolic compound in HG-PE was narcissin (7.4%), HP-PE shared 3 major constituents, namely <i>cis</i>-2-hydroxy-4-methoxycinnamic acid 2-<i>O</i>-β-glucoside (<i>cis</i>-GMCA, 5.8%), narcissin (5.4%), and rutin (5.3%). Almost half of HIh phenolic content (14.7%) belonged to oxytroflavoside A (7-<i>O</i>-methylkaempferol-3-<i>O</i>-[3-hydroxy-3-methylglutaryl-(1→6)]-[α-rhamnopyranosyl-(1→2)]-β-galactopyranoside). Antioxidant properties of the <i>Herniaria</i> PEs were evaluated employing an experimental model of human blood plasma, exposed to the peroxynitrite-induced oxidative stress. The assays demonstrated significant reduction of oxidative damage to protein and lipid plasma components (estimated by measurements of 3-nitrotyrosine, protein thiol groups, thiobarbituric acid-reactive substances), and moderate protection of its non-enzymatic antioxidant capacity. Anti-inflammatory properties of the <i>Herniaria</i> PEs were evaluated In Vitro as inhibitory effects against cyclooxygenases (COX-1 and -2) and concanavalin A-induced inflammatory response of the peripheral blood mononuclear cells (PBMCs). None of the studied plants showed inhibitory effects on COXs but all purified extracts partly reduced the release of interleukin 2 (IL-2) and tumor necrosis factor-alpha (TNF-α) from PBMCs, which suggested their prospective ability to up-regulate inflammatory response of the cells. The purified extract from <i>H. glabra</i> turned out to be the most efficient suppressor of PBMCs’ inflammatory response. Additionally, cytotoxicity of purified <i>Herniaria</i> extracts on PBMCs was ruled out based on In Vitro studies. |
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| spelling | doaj.art-18a51e34444f432e8306ac333d61334b2023-11-23T14:53:21ZengMDPI AGMolecules1420-30492022-01-0127253010.3390/molecules27020530Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing <i>Herniaria</i> L. Species and Their Antioxidant and Anti-Inflammatory Activities In VitroSolomiia Kozachok0Joanna Kolodziejczyk-Czepas1Svitlana Marchyshyn2Krzysztof Kamil Wojtanowski3Grażyna Zgórka4Wieslaw Oleszek5Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation—State Research Institute, Czartoryskich 8, 24-100 Puławy, PolandDepartment of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, PolandDepartment of Pharmacognosy and Medical Botany, I Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001 Ternopil, UkraineDepartment of Pharmacognosy with the Medicinal Plant Garden, Medical University of Lublin, 1 Chodzki St., 20-093 Lublin, PolandDepartment of Pharmacognosy with the Medicinal Plant Garden, Medical University of Lublin, 1 Chodzki St., 20-093 Lublin, PolandDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation—State Research Institute, Czartoryskich 8, 24-100 Puławy, PolandThe work is aimed at phytochemical characterization and In Vitro evaluation of antioxidant actions, anti-inflammatory effects, and cytotoxicity of purified extracts from three rupturewort (<i>Herniaria</i> L.) species, i.e., <i>Herniaria glabra</i> (HG), <i>H. polygama</i> (HP), and <i>H. incana</i> herb (HIh). The total phenolic content established in the purified extracts (PEs) of HIh, HP, and HG was 29.6, 24.0, and 13.0%, respectively. Thirty-eight non-saponin metabolites were identified using LC-HR-QTOF-ESI-MS; however, only 9 were common for the studied <i>Herniaria</i> species. The most abundant phenolic compound in HG-PE was narcissin (7.4%), HP-PE shared 3 major constituents, namely <i>cis</i>-2-hydroxy-4-methoxycinnamic acid 2-<i>O</i>-β-glucoside (<i>cis</i>-GMCA, 5.8%), narcissin (5.4%), and rutin (5.3%). Almost half of HIh phenolic content (14.7%) belonged to oxytroflavoside A (7-<i>O</i>-methylkaempferol-3-<i>O</i>-[3-hydroxy-3-methylglutaryl-(1→6)]-[α-rhamnopyranosyl-(1→2)]-β-galactopyranoside). Antioxidant properties of the <i>Herniaria</i> PEs were evaluated employing an experimental model of human blood plasma, exposed to the peroxynitrite-induced oxidative stress. The assays demonstrated significant reduction of oxidative damage to protein and lipid plasma components (estimated by measurements of 3-nitrotyrosine, protein thiol groups, thiobarbituric acid-reactive substances), and moderate protection of its non-enzymatic antioxidant capacity. Anti-inflammatory properties of the <i>Herniaria</i> PEs were evaluated In Vitro as inhibitory effects against cyclooxygenases (COX-1 and -2) and concanavalin A-induced inflammatory response of the peripheral blood mononuclear cells (PBMCs). None of the studied plants showed inhibitory effects on COXs but all purified extracts partly reduced the release of interleukin 2 (IL-2) and tumor necrosis factor-alpha (TNF-α) from PBMCs, which suggested their prospective ability to up-regulate inflammatory response of the cells. The purified extract from <i>H. glabra</i> turned out to be the most efficient suppressor of PBMCs’ inflammatory response. Additionally, cytotoxicity of purified <i>Herniaria</i> extracts on PBMCs was ruled out based on In Vitro studies.https://www.mdpi.com/1420-3049/27/2/530<i>Herniaria glabra</i> L.<i>H. polygama</i> J. Gay.<i>H. incana</i> Lam.CaryophyllaceaeLC-MSphenolic metabolites |
| spellingShingle | Solomiia Kozachok Joanna Kolodziejczyk-Czepas Svitlana Marchyshyn Krzysztof Kamil Wojtanowski Grażyna Zgórka Wieslaw Oleszek Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing <i>Herniaria</i> L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro Molecules <i>Herniaria glabra</i> L. <i>H. polygama</i> J. Gay. <i>H. incana</i> Lam. Caryophyllaceae LC-MS phenolic metabolites |
| title | Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing <i>Herniaria</i> L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro |
| title_full | Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing <i>Herniaria</i> L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro |
| title_fullStr | Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing <i>Herniaria</i> L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro |
| title_full_unstemmed | Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing <i>Herniaria</i> L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro |
| title_short | Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing <i>Herniaria</i> L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro |
| title_sort | comparison of phenolic metabolites in purified extracts of three wild growing i herniaria i l species and their antioxidant and anti inflammatory activities in vitro |
| topic | <i>Herniaria glabra</i> L. <i>H. polygama</i> J. Gay. <i>H. incana</i> Lam. Caryophyllaceae LC-MS phenolic metabolites |
| url | https://www.mdpi.com/1420-3049/27/2/530 |
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