Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio
Aim: The study was conducted to investigate the effect of cholesterol loaded cyclodextrin (CLC) on freezability of buffalo spermatozoa. Materials and Methods: Murrah buffalo bull semen samples with progressive motility of 70% and greater were used. After the evaluation of motility and livability, f...
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| Format: | Article |
| Language: | English |
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Veterinary World
2014-09-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.7/September-2014/15.pdf |
| _version_ | 1818741026635907072 |
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| author | J. S. Rajoriya J. K. Prasad S. K. Ghosh Snehal S. Ramteke N. C. Barik G. K. Das Megha Pande |
| author_facet | J. S. Rajoriya J. K. Prasad S. K. Ghosh Snehal S. Ramteke N. C. Barik G. K. Das Megha Pande |
| author_sort | J. S. Rajoriya |
| collection | DOAJ |
| description | Aim: The study was conducted to investigate the effect of cholesterol loaded cyclodextrin (CLC) on freezability of buffalo spermatozoa.
Materials and Methods: Murrah buffalo bull semen samples with progressive motility of 70% and greater were used. After the evaluation of motility and livability, four equal fractions of semen samples were made. Group I was kept as control and diluted with Tris, whereas Group II, III and IV were treated with CLC solution at the rate of 2.0, 3.0 and 4.0 mg/ml respectively to obtain 120 × 106 sperm/ml as final spermatozoa concentration. The aliquots of all the groups were incubated for action of CLC, followed by dilution and freezing. Evaluation at pre-freeze and post-thaw stage of progressive motility, viability and level of cholesterol and phospholipid was done.
Results: The mean cholesterol content (μg/100 × 106 spermatozoa) of Group I, II, III and IV at pre-freeze stage was 21.55±0.63, 49.56±1.38, 55.67±0.45 and 47.79±1.01 and at post-thaw stage were 13.18±0.45, 34.27±0.71, 36.21±0.48 and 33.68±0.56, respectively. At pre-freeze stage, cholesterol content was significantly (p<0.01) higher in Group III in comparison to other groups. The mean cholesterol and phospholipids content of fresh sperm was 24.14±0.58 and 51.13±0.66 μg/100 × 106 sperm cells, respectively, and C/P ratio of spermatozoa at fresh stage was 0.47±0.067.
Conclusion: CLC treatment maintains the C/P ratio and plays an important role in maintaining membrane architecture of spermatozoa. Hence, addition of CLC may be helpful in increasing freezability of buffalo spermatozoa by increasing the C/P ratio of spermatozoa. |
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| last_indexed | 2024-12-18T01:50:05Z |
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| spelling | doaj.art-18a8002bffbe4374a3a1b0a5dae5c6752022-12-21T21:25:05ZengVeterinary WorldVeterinary World0972-89882231-09162014-09-017970270610.14202/vetworld.2014.702-706Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratioJ. S. Rajoriya0J. K. Prasad1S. K. Ghosh2Snehal S. Ramteke3N. C. Barik4G. K. Das 5Megha Pande6Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; jituivri@gmail.comGerm Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; jkprasad2001@yahoo.comGerm Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; subrata_kghosh@yahoo.co.inGerm Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; drsnehalramteke@rediffmail.comGerm Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; iamdrncbarik@gmail.comGerm Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; gkdasivri@gmail.comAnimal Physiology Lab, Central Institute for Research on Cattle, Meerut Cantt, Uttar Pradesh, India; megha985@gmail.comAim: The study was conducted to investigate the effect of cholesterol loaded cyclodextrin (CLC) on freezability of buffalo spermatozoa. Materials and Methods: Murrah buffalo bull semen samples with progressive motility of 70% and greater were used. After the evaluation of motility and livability, four equal fractions of semen samples were made. Group I was kept as control and diluted with Tris, whereas Group II, III and IV were treated with CLC solution at the rate of 2.0, 3.0 and 4.0 mg/ml respectively to obtain 120 × 106 sperm/ml as final spermatozoa concentration. The aliquots of all the groups were incubated for action of CLC, followed by dilution and freezing. Evaluation at pre-freeze and post-thaw stage of progressive motility, viability and level of cholesterol and phospholipid was done. Results: The mean cholesterol content (μg/100 × 106 spermatozoa) of Group I, II, III and IV at pre-freeze stage was 21.55±0.63, 49.56±1.38, 55.67±0.45 and 47.79±1.01 and at post-thaw stage were 13.18±0.45, 34.27±0.71, 36.21±0.48 and 33.68±0.56, respectively. At pre-freeze stage, cholesterol content was significantly (p<0.01) higher in Group III in comparison to other groups. The mean cholesterol and phospholipids content of fresh sperm was 24.14±0.58 and 51.13±0.66 μg/100 × 106 sperm cells, respectively, and C/P ratio of spermatozoa at fresh stage was 0.47±0.067. Conclusion: CLC treatment maintains the C/P ratio and plays an important role in maintaining membrane architecture of spermatozoa. Hence, addition of CLC may be helpful in increasing freezability of buffalo spermatozoa by increasing the C/P ratio of spermatozoa.http://www.veterinaryworld.org/Vol.7/September-2014/15.pdfbuffalocholesterol loaded cyclodextrincholesterol:phospholipid (C/P) ratio |
| spellingShingle | J. S. Rajoriya J. K. Prasad S. K. Ghosh Snehal S. Ramteke N. C. Barik G. K. Das Megha Pande Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio Veterinary World buffalo cholesterol loaded cyclodextrin cholesterol:phospholipid (C/P) ratio |
| title | Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio |
| title_full | Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio |
| title_fullStr | Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio |
| title_full_unstemmed | Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio |
| title_short | Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio |
| title_sort | cholesterol loaded cyclodextrin increases freezability of buffalo bull bubalus bubalis spermatozoa by increasing cholesterol to phospholipid ratio |
| topic | buffalo cholesterol loaded cyclodextrin cholesterol:phospholipid (C/P) ratio |
| url | http://www.veterinaryworld.org/Vol.7/September-2014/15.pdf |
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