Network analysis of the proteome and peptidome sheds light on human milk as a biological system
Abstract Proteins and peptides found in human milk have bioactive potential to benefit the newborn and support healthy development. Research has been carried out on the health benefits of proteins and peptides, but many questions still need to be answered about the nature of these components, how th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-58127-2 |
| _version_ | 1797233575514341376 |
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| author | Pieter M. Dekker Sjef Boeren Edoardo Saccenti Kasper A. Hettinga |
| author_facet | Pieter M. Dekker Sjef Boeren Edoardo Saccenti Kasper A. Hettinga |
| author_sort | Pieter M. Dekker |
| collection | DOAJ |
| description | Abstract Proteins and peptides found in human milk have bioactive potential to benefit the newborn and support healthy development. Research has been carried out on the health benefits of proteins and peptides, but many questions still need to be answered about the nature of these components, how they are formed, and how they end up in the milk. This study explored and elucidated the complexity of the human milk proteome and peptidome. Proteins and peptides were analyzed with non-targeted nanoLC-Orbitrap-MS/MS in a selection of 297 milk samples from the CHILD Cohort Study. Protein and peptide abundances were determined, and a network was inferred using Gaussian graphical modeling (GGM), allowing an investigation of direct associations. This study showed that signatures of (1) specific mechanisms of transport of different groups of proteins, (2) proteolytic degradation by proteases and aminopeptidases, and (3) coagulation and complement activation are present in human milk. These results show the value of an integrated approach in evaluating large-scale omics data sets and provide valuable information for studies that aim to associate protein or peptide profiles from biofluids such as milk with specific physiological characteristics. |
| first_indexed | 2024-04-24T16:18:21Z |
| format | Article |
| id | doaj.art-18a848e3f2c940b3a34e6c9e56a4f09c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T16:18:21Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-18a848e3f2c940b3a34e6c9e56a4f09c2024-03-31T11:18:56ZengNature PortfolioScientific Reports2045-23222024-03-0114111310.1038/s41598-024-58127-2Network analysis of the proteome and peptidome sheds light on human milk as a biological systemPieter M. Dekker0Sjef Boeren1Edoardo Saccenti2Kasper A. Hettinga3Food Quality and Design Group, Wageningen University and ResearchLaboratory of Biochemistry, Wageningen University and ResearchLaboratory of Systems and Synthetic Biology, Wageningen University and ResearchFood Quality and Design Group, Wageningen University and ResearchAbstract Proteins and peptides found in human milk have bioactive potential to benefit the newborn and support healthy development. Research has been carried out on the health benefits of proteins and peptides, but many questions still need to be answered about the nature of these components, how they are formed, and how they end up in the milk. This study explored and elucidated the complexity of the human milk proteome and peptidome. Proteins and peptides were analyzed with non-targeted nanoLC-Orbitrap-MS/MS in a selection of 297 milk samples from the CHILD Cohort Study. Protein and peptide abundances were determined, and a network was inferred using Gaussian graphical modeling (GGM), allowing an investigation of direct associations. This study showed that signatures of (1) specific mechanisms of transport of different groups of proteins, (2) proteolytic degradation by proteases and aminopeptidases, and (3) coagulation and complement activation are present in human milk. These results show the value of an integrated approach in evaluating large-scale omics data sets and provide valuable information for studies that aim to associate protein or peptide profiles from biofluids such as milk with specific physiological characteristics.https://doi.org/10.1038/s41598-024-58127-2 |
| spellingShingle | Pieter M. Dekker Sjef Boeren Edoardo Saccenti Kasper A. Hettinga Network analysis of the proteome and peptidome sheds light on human milk as a biological system Scientific Reports |
| title | Network analysis of the proteome and peptidome sheds light on human milk as a biological system |
| title_full | Network analysis of the proteome and peptidome sheds light on human milk as a biological system |
| title_fullStr | Network analysis of the proteome and peptidome sheds light on human milk as a biological system |
| title_full_unstemmed | Network analysis of the proteome and peptidome sheds light on human milk as a biological system |
| title_short | Network analysis of the proteome and peptidome sheds light on human milk as a biological system |
| title_sort | network analysis of the proteome and peptidome sheds light on human milk as a biological system |
| url | https://doi.org/10.1038/s41598-024-58127-2 |
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