Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta
The purpose of this study was to clarify the structural and ultrastructural alterations of the enamel and dentin collagen network in the deciduous teeth of children affected by osteogenesis imperfecta (OI) using field-emission in-lens scanning electron microscopy (FEI-SEM) and transmission electron...
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MDPI AG
2020-11-01
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| Online Access: | https://www.mdpi.com/2076-3417/10/21/7835 |
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| author | Uros Josic Tatjana Maravic Maurizio Bossù Milena Cadenaro Allegra Comba Gaetano Ierardo Antonella Polimeni Federica Florenzano Lorenzo Breschi Annalisa Mazzoni |
| author_facet | Uros Josic Tatjana Maravic Maurizio Bossù Milena Cadenaro Allegra Comba Gaetano Ierardo Antonella Polimeni Federica Florenzano Lorenzo Breschi Annalisa Mazzoni |
| author_sort | Uros Josic |
| collection | DOAJ |
| description | The purpose of this study was to clarify the structural and ultrastructural alterations of the enamel and dentin collagen network in the deciduous teeth of children affected by osteogenesis imperfecta (OI) using field-emission in-lens scanning electron microscopy (FEI-SEM) and transmission electron microscopy (TEM) analyses. Exfoliated primary teeth were collected from children with a diagnosis of OI and from healthy individuals (<i>N</i> = 24). Tooth slices containing both dentin and enamel were fixed, dehydrated and dried, gold sputtered, and observed using FEI-SEM. Additional dentin fragments were decalcified, dehydrated, embedded in resin, cut, and processed for TEM analysis. Under FEI-SEM, the enamel in OI-affected children showed an irregular prism distribution with the enamel hydroxyapatite crystals unpacked. Ultrastructural correlative analysis of the dentin in patients affected by OI showed an altered collagen pattern with a low density. In some areas, teeth in OI patients showed a reduction in the number of dentinal tubules, with odontoblastic process missing in most of the tubules. The presence of altered dentine and enamel organization in OI children was firmly established at an ultrastructural level, but additional biochemical studies are necessary in order to clarify quantitatively and qualitatively the collagenic and non-collagenic proteins in this disorder. |
| first_indexed | 2024-03-10T15:04:46Z |
| format | Article |
| id | doaj.art-18aa4d806bb84990b1ae431bc3e27379 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T15:04:46Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-18aa4d806bb84990b1ae431bc3e273792023-11-20T19:49:53ZengMDPI AGApplied Sciences2076-34172020-11-011021783510.3390/app10217835Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis ImperfectaUros Josic0Tatjana Maravic1Maurizio Bossù2Milena Cadenaro3Allegra Comba4Gaetano Ierardo5Antonella Polimeni6Federica Florenzano7Lorenzo Breschi8Annalisa Mazzoni9Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, 40125 Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, 40125 Bologna, ItalyDepartment of Oral and Maxillofacial Science, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Medical Sciences, University of Trieste, 34149 Trieste, ItalyDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, 40125 Bologna, ItalyDepartment of Oral and Maxillofacial Science, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Oral and Maxillofacial Science, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, 40125 Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, 40125 Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, 40125 Bologna, ItalyThe purpose of this study was to clarify the structural and ultrastructural alterations of the enamel and dentin collagen network in the deciduous teeth of children affected by osteogenesis imperfecta (OI) using field-emission in-lens scanning electron microscopy (FEI-SEM) and transmission electron microscopy (TEM) analyses. Exfoliated primary teeth were collected from children with a diagnosis of OI and from healthy individuals (<i>N</i> = 24). Tooth slices containing both dentin and enamel were fixed, dehydrated and dried, gold sputtered, and observed using FEI-SEM. Additional dentin fragments were decalcified, dehydrated, embedded in resin, cut, and processed for TEM analysis. Under FEI-SEM, the enamel in OI-affected children showed an irregular prism distribution with the enamel hydroxyapatite crystals unpacked. Ultrastructural correlative analysis of the dentin in patients affected by OI showed an altered collagen pattern with a low density. In some areas, teeth in OI patients showed a reduction in the number of dentinal tubules, with odontoblastic process missing in most of the tubules. The presence of altered dentine and enamel organization in OI children was firmly established at an ultrastructural level, but additional biochemical studies are necessary in order to clarify quantitatively and qualitatively the collagenic and non-collagenic proteins in this disorder.https://www.mdpi.com/2076-3417/10/21/7835deciduous teethdentinogenesis imperfectaosteogenesis imperfectaenameldentinultrastructural diagnosis |
| spellingShingle | Uros Josic Tatjana Maravic Maurizio Bossù Milena Cadenaro Allegra Comba Gaetano Ierardo Antonella Polimeni Federica Florenzano Lorenzo Breschi Annalisa Mazzoni Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta Applied Sciences deciduous teeth dentinogenesis imperfecta osteogenesis imperfecta enamel dentin ultrastructural diagnosis |
| title | Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta |
| title_full | Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta |
| title_fullStr | Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta |
| title_full_unstemmed | Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta |
| title_short | Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta |
| title_sort | morphological characterization of deciduous enamel and dentin in patients affected by osteogenesis imperfecta |
| topic | deciduous teeth dentinogenesis imperfecta osteogenesis imperfecta enamel dentin ultrastructural diagnosis |
| url | https://www.mdpi.com/2076-3417/10/21/7835 |
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