Analysis of the Impact of Ethanol Extract of <i>Calendula officinalis</i> L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells
<i>Calendula officinalis</i> L. promotes wound healing and might be effective in gingival fibroblast stimulation. The influence of different concentrations of <i>Calendula officinalis</i> L. ethanol extract on human gingival fibroblast was visualized using PANsys 3000—a fully...
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MDPI AG
2023-09-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/10/1949 |
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| author | Agnieszka Nowak-Terpiłowska Izabela Nowak Agnieszka Feliczak-Guzik Marzena Wyganowska |
| author_facet | Agnieszka Nowak-Terpiłowska Izabela Nowak Agnieszka Feliczak-Guzik Marzena Wyganowska |
| author_sort | Agnieszka Nowak-Terpiłowska |
| collection | DOAJ |
| description | <i>Calendula officinalis</i> L. promotes wound healing and might be effective in gingival fibroblast stimulation. The influence of different concentrations of <i>Calendula officinalis</i> L. ethanol extract on human gingival fibroblast was visualized using PANsys 3000—a fully automated cell culture device used for in vitro culture to study cells under conditions similar to in vivo. The human fibroblast cells were isolated from gingival tissue. The 100% brew of <i>Calendula officinalis</i> L., as well as 7% and 20% <i>Calendula officinalis</i> L. ethanol extract, were added to the cultured cells and observed for 72 h. The qualitative and quantitative composition of the volatile compounds of marigold <i>Calendula officinalis</i> L. flowers are presented in this study. The essential oil compounds of the decoction were isolated with solid-phase microextraction (SPME) and analyzed with gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The presence of terpenoids, flavonoids, and other compounds was demonstrated. The composition was correlated with the fragrance properties. Observation of gingival fibroblast showed that there were no changes in cell morphology and proliferation after 100% <i>Calendula officinalis</i> L. brew stimulation. The growth and cell division were not inhibited. Likewise, the addition of 7% or 20% ethanol in water extract of <i>Calendula officinalis</i> L. stimulation did not inhibit the fibroblast proliferation. Overall, ethanol extracts of <i>Calendula officinalis</i> L. decrease the alcohol cytotoxic influence on gingival fibroblasts. |
| first_indexed | 2024-03-10T21:06:36Z |
| format | Article |
| id | doaj.art-18aac573b4b2465ab8d03a0738da6938 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T21:06:36Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-18aac573b4b2465ab8d03a0738da69382023-11-19T17:05:13ZengMDPI AGLife2075-17292023-09-011310194910.3390/life13101949Analysis of the Impact of Ethanol Extract of <i>Calendula officinalis</i> L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of CellsAgnieszka Nowak-Terpiłowska0Izabela Nowak1Agnieszka Feliczak-Guzik2Marzena Wyganowska3Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11 St., 60-632 Poznan, PolandDepartment of Applied Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8 St., 61-614 Poznan, PolandDepartment of Applied Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8 St., 61-614 Poznan, PolandDepartment of Dental Surgery, Periodontology and Oral Mucosa Diseases, Poznan University of Medical Sciences, Bukowska 70 St., 60-812 Poznan, Poland<i>Calendula officinalis</i> L. promotes wound healing and might be effective in gingival fibroblast stimulation. The influence of different concentrations of <i>Calendula officinalis</i> L. ethanol extract on human gingival fibroblast was visualized using PANsys 3000—a fully automated cell culture device used for in vitro culture to study cells under conditions similar to in vivo. The human fibroblast cells were isolated from gingival tissue. The 100% brew of <i>Calendula officinalis</i> L., as well as 7% and 20% <i>Calendula officinalis</i> L. ethanol extract, were added to the cultured cells and observed for 72 h. The qualitative and quantitative composition of the volatile compounds of marigold <i>Calendula officinalis</i> L. flowers are presented in this study. The essential oil compounds of the decoction were isolated with solid-phase microextraction (SPME) and analyzed with gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The presence of terpenoids, flavonoids, and other compounds was demonstrated. The composition was correlated with the fragrance properties. Observation of gingival fibroblast showed that there were no changes in cell morphology and proliferation after 100% <i>Calendula officinalis</i> L. brew stimulation. The growth and cell division were not inhibited. Likewise, the addition of 7% or 20% ethanol in water extract of <i>Calendula officinalis</i> L. stimulation did not inhibit the fibroblast proliferation. Overall, ethanol extracts of <i>Calendula officinalis</i> L. decrease the alcohol cytotoxic influence on gingival fibroblasts.https://www.mdpi.com/2075-1729/13/10/1949<i>Calendula officinalis</i> L.fibroblastsmorphologyproliferation |
| spellingShingle | Agnieszka Nowak-Terpiłowska Izabela Nowak Agnieszka Feliczak-Guzik Marzena Wyganowska Analysis of the Impact of Ethanol Extract of <i>Calendula officinalis</i> L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells Life <i>Calendula officinalis</i> L. fibroblasts morphology proliferation |
| title | Analysis of the Impact of Ethanol Extract of <i>Calendula officinalis</i> L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells |
| title_full | Analysis of the Impact of Ethanol Extract of <i>Calendula officinalis</i> L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells |
| title_fullStr | Analysis of the Impact of Ethanol Extract of <i>Calendula officinalis</i> L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells |
| title_full_unstemmed | Analysis of the Impact of Ethanol Extract of <i>Calendula officinalis</i> L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells |
| title_short | Analysis of the Impact of Ethanol Extract of <i>Calendula officinalis</i> L. on Human Fibroblast Cell Cultures Using the PANsys 3000 Device for Breeding and Visualization of Cells |
| title_sort | analysis of the impact of ethanol extract of i calendula officinalis i l on human fibroblast cell cultures using the pansys 3000 device for breeding and visualization of cells |
| topic | <i>Calendula officinalis</i> L. fibroblasts morphology proliferation |
| url | https://www.mdpi.com/2075-1729/13/10/1949 |
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