Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA Profiling
This study aimed to identify novel biomarkers for metastatic colorectal cancer progression using exosomal RNA expression profiling. The exosomal RNA expression profiles of 54 patients with mCRC were investigated. Exosomal RNA profiling was performed at the time of relapse immediately before metastas...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/14/19/4723 |
| _version_ | 1797480251829256192 |
|---|---|
| author | Soohyeon Lee Young Soo Park Jwa Hoon Kim Ah Reum Lim Myung Han Hyun Boyeon Kim Jong Won Lee Saet Byeol Lee Yeul Hong Kim |
| author_facet | Soohyeon Lee Young Soo Park Jwa Hoon Kim Ah Reum Lim Myung Han Hyun Boyeon Kim Jong Won Lee Saet Byeol Lee Yeul Hong Kim |
| author_sort | Soohyeon Lee |
| collection | DOAJ |
| description | This study aimed to identify novel biomarkers for metastatic colorectal cancer progression using exosomal RNA expression profiling. The exosomal RNA expression profiles of 54 patients with mCRC were investigated. Exosomal RNA profiling was performed at the time of relapse immediately before metastasectomy and cancer recurrence or progression after metastasectomy. The up- and down-regulated RNA expression profiles were screened and analyzed using H-cluster, principle component analysis and gene ontology. The tissue expression profile of the liver metastases was compared with the GSE 41258 set using GSEA tools. We identified two distinctive biological process gene sets (IFNA and PCDB families) related to metastatic progression. The interferon-α response gene set was enriched, especially when the tumor volume was ≥1 cm<sup>3</sup>. CXCL10, CXCL11 and SAMD 9 mRNA were highly expressed in the plasma exosome samples of patients with mCRC to the liver. Furthermore, high expression of CXCL10 but not CXCL11 or SAMD9 was associated with a poor prognosis and shorter progression-free survival. Conclusions: Cancer-derived exosomal CXCL10 may be a novel biomarker for liver metastasis of mCRC and a potential target for the prevention and treatment of mCRC with liver metastasis. |
| first_indexed | 2024-03-09T21:57:20Z |
| format | Article |
| id | doaj.art-18ab9fe106c649238199fd3a892297ac |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T21:57:20Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-18ab9fe106c649238199fd3a892297ac2023-11-23T19:55:31ZengMDPI AGCancers2072-66942022-09-011419472310.3390/cancers14194723Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA ProfilingSoohyeon Lee0Young Soo Park1Jwa Hoon Kim2Ah Reum Lim3Myung Han Hyun4Boyeon Kim5Jong Won Lee6Saet Byeol Lee7Yeul Hong Kim8Division of Medical Oncology and Hematology, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaCancer Research Institute, Korea University College of Medicine, Seoul 02841, KoreaDivision of Medical Oncology and Hematology, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaDivision of Medical Oncology and Hematology, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaDivision of Medical Oncology and Hematology, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaCancer Research Institute, Korea University College of Medicine, Seoul 02841, KoreaCancer Research Institute, Korea University College of Medicine, Seoul 02841, KoreaCancer Research Institute, Korea University College of Medicine, Seoul 02841, KoreaDivision of Medical Oncology and Hematology, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaThis study aimed to identify novel biomarkers for metastatic colorectal cancer progression using exosomal RNA expression profiling. The exosomal RNA expression profiles of 54 patients with mCRC were investigated. Exosomal RNA profiling was performed at the time of relapse immediately before metastasectomy and cancer recurrence or progression after metastasectomy. The up- and down-regulated RNA expression profiles were screened and analyzed using H-cluster, principle component analysis and gene ontology. The tissue expression profile of the liver metastases was compared with the GSE 41258 set using GSEA tools. We identified two distinctive biological process gene sets (IFNA and PCDB families) related to metastatic progression. The interferon-α response gene set was enriched, especially when the tumor volume was ≥1 cm<sup>3</sup>. CXCL10, CXCL11 and SAMD 9 mRNA were highly expressed in the plasma exosome samples of patients with mCRC to the liver. Furthermore, high expression of CXCL10 but not CXCL11 or SAMD9 was associated with a poor prognosis and shorter progression-free survival. Conclusions: Cancer-derived exosomal CXCL10 may be a novel biomarker for liver metastasis of mCRC and a potential target for the prevention and treatment of mCRC with liver metastasis.https://www.mdpi.com/2072-6694/14/19/4723liquid biopsyexosomal RNAmetastatic colorectal cancerliver metastasistherapy resistance |
| spellingShingle | Soohyeon Lee Young Soo Park Jwa Hoon Kim Ah Reum Lim Myung Han Hyun Boyeon Kim Jong Won Lee Saet Byeol Lee Yeul Hong Kim Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA Profiling Cancers liquid biopsy exosomal RNA metastatic colorectal cancer liver metastasis therapy resistance |
| title | Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA Profiling |
| title_full | Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA Profiling |
| title_fullStr | Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA Profiling |
| title_full_unstemmed | Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA Profiling |
| title_short | Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA Profiling |
| title_sort | identification of biomarkers associated with liver metastasis progression from colorectal cancer using exosomal rna profiling |
| topic | liquid biopsy exosomal RNA metastatic colorectal cancer liver metastasis therapy resistance |
| url | https://www.mdpi.com/2072-6694/14/19/4723 |
| work_keys_str_mv | AT soohyeonlee identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling AT youngsoopark identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling AT jwahoonkim identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling AT ahreumlim identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling AT myunghanhyun identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling AT boyeonkim identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling AT jongwonlee identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling AT saetbyeollee identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling AT yeulhongkim identificationofbiomarkersassociatedwithlivermetastasisprogressionfromcolorectalcancerusingexosomalrnaprofiling |