Analysis of the utilization value of different tissues of Taxus×Media based on metabolomics and antioxidant activity
Abstract Background Taxaceae, is a class of dioecious and evergreen plant with substantial economic and ecology value. At present many phytochemical analyses have been performed in Taxus plants. And various biological constituents have been isolated from various Taxus species. However, the differenc...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04308-6 |
| _version_ | 1797811525254119424 |
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| author | Meng Li WanRu Geng Zhi Wang Qian Wang Lei Pang Baoyi Wang PeiQiang Wang FengFeng Qu XinFu Zhang |
| author_facet | Meng Li WanRu Geng Zhi Wang Qian Wang Lei Pang Baoyi Wang PeiQiang Wang FengFeng Qu XinFu Zhang |
| author_sort | Meng Li |
| collection | DOAJ |
| description | Abstract Background Taxaceae, is a class of dioecious and evergreen plant with substantial economic and ecology value. At present many phytochemical analyses have been performed in Taxus plants. And various biological constituents have been isolated from various Taxus species. However, the difference of compounds and antioxidant capacity of different tissues of T. media is not clear. Results In the present study, we investigated the metabolites and antioxidant activity of four tissues of T. media, including T. media bark (TB), T. media fresh leaves (TFL), T. media seeds (TS), T. media aril (TA). In total, 808 compounds, covering 11 subclasses, were identified by using UPLC-MS/MS. Paclitaxel, the most popular anticancer compound, was found to accumulate most in TS, followed by TB, TFL and TA in order. Further analysis found that 70 key differential metabolites with VIP > 1.0 and p < 0.05, covering 8 subclasses, were screened as the key differential metabolites in four tissues. The characteristic compounds of TFL mainly included flavonoids and tanninsis. Alkaloids and phenolic acids were major characteristic compounds of TS and TB respectively. Amino acids and derivatives, organic acids, saccharides and lipids were the major characteristic compounds of TA. Additionally, based on FRAP and ABTS method, TS and TFL exhibited higher antioxidant activity than TB and TA. Conclusion There was significant difference in metabolite content among different tissues of T. media. TFL and TS had higher metabolites and antioxidant capacity than other tissues, indicating that TFL and TS were more suitable for the development and utilization of T. media in foods and drinks. |
| first_indexed | 2024-03-13T07:25:00Z |
| format | Article |
| id | doaj.art-18ae15ae6d744fff8a5c6dbec2fbf0e5 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-13T07:25:00Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-18ae15ae6d744fff8a5c6dbec2fbf0e52023-06-04T11:24:53ZengBMCBMC Plant Biology1471-22292023-05-0123111010.1186/s12870-023-04308-6Analysis of the utilization value of different tissues of Taxus×Media based on metabolomics and antioxidant activityMeng Li0WanRu Geng1Zhi Wang2Qian Wang3Lei Pang4Baoyi Wang5PeiQiang Wang6FengFeng Qu7XinFu Zhang8College of Horticulture, Qingdao Agricultural UniversityGraduate School, University of JinanCollege of Horticulture, Qingdao Agricultural UniversityCollege of Horticulture, Qingdao Agricultural UniversityArchives, Shandong University of TechnologyCollege of Horticulture, Qingdao Agricultural UniversityCollege of Horticulture, Qingdao Agricultural UniversityCollege of Horticulture, Qingdao Agricultural UniversityCollege of Horticulture, Qingdao Agricultural UniversityAbstract Background Taxaceae, is a class of dioecious and evergreen plant with substantial economic and ecology value. At present many phytochemical analyses have been performed in Taxus plants. And various biological constituents have been isolated from various Taxus species. However, the difference of compounds and antioxidant capacity of different tissues of T. media is not clear. Results In the present study, we investigated the metabolites and antioxidant activity of four tissues of T. media, including T. media bark (TB), T. media fresh leaves (TFL), T. media seeds (TS), T. media aril (TA). In total, 808 compounds, covering 11 subclasses, were identified by using UPLC-MS/MS. Paclitaxel, the most popular anticancer compound, was found to accumulate most in TS, followed by TB, TFL and TA in order. Further analysis found that 70 key differential metabolites with VIP > 1.0 and p < 0.05, covering 8 subclasses, were screened as the key differential metabolites in four tissues. The characteristic compounds of TFL mainly included flavonoids and tanninsis. Alkaloids and phenolic acids were major characteristic compounds of TS and TB respectively. Amino acids and derivatives, organic acids, saccharides and lipids were the major characteristic compounds of TA. Additionally, based on FRAP and ABTS method, TS and TFL exhibited higher antioxidant activity than TB and TA. Conclusion There was significant difference in metabolite content among different tissues of T. media. TFL and TS had higher metabolites and antioxidant capacity than other tissues, indicating that TFL and TS were more suitable for the development and utilization of T. media in foods and drinks.https://doi.org/10.1186/s12870-023-04308-6T. mediaWidely targeted metabolomicsMetabolitesDifferential metabolitesAntioxidant activity |
| spellingShingle | Meng Li WanRu Geng Zhi Wang Qian Wang Lei Pang Baoyi Wang PeiQiang Wang FengFeng Qu XinFu Zhang Analysis of the utilization value of different tissues of Taxus×Media based on metabolomics and antioxidant activity BMC Plant Biology T. media Widely targeted metabolomics Metabolites Differential metabolites Antioxidant activity |
| title | Analysis of the utilization value of different tissues of Taxus×Media based on metabolomics and antioxidant activity |
| title_full | Analysis of the utilization value of different tissues of Taxus×Media based on metabolomics and antioxidant activity |
| title_fullStr | Analysis of the utilization value of different tissues of Taxus×Media based on metabolomics and antioxidant activity |
| title_full_unstemmed | Analysis of the utilization value of different tissues of Taxus×Media based on metabolomics and antioxidant activity |
| title_short | Analysis of the utilization value of different tissues of Taxus×Media based on metabolomics and antioxidant activity |
| title_sort | analysis of the utilization value of different tissues of taxus media based on metabolomics and antioxidant activity |
| topic | T. media Widely targeted metabolomics Metabolites Differential metabolites Antioxidant activity |
| url | https://doi.org/10.1186/s12870-023-04308-6 |
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