Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, Iraq
The current work was conducted to unveil the current situation for the infection by<em> Theileria annulata</em> in cattle in Al-Hilla City, Iraq. A total of 225 blood samples (200 from suspected infected animals and 25 from clinically healthy animals as a control group) were collected. T...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2023-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_177329_5570cea502f844ddc2ee34acc897dc5f.pdf |
| _version_ | 1797854712909791232 |
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| author | Ayat Q. Jawad Monyer A. Al-Fatlawi |
| author_facet | Ayat Q. Jawad Monyer A. Al-Fatlawi |
| author_sort | Ayat Q. Jawad |
| collection | DOAJ |
| description | The current work was conducted to unveil the current situation for the infection by<em> Theileria annulata</em> in cattle in Al-Hilla City, Iraq. A total of 225 blood samples (200 from suspected infected animals and 25 from clinically healthy animals as a control group) were collected. These samples were subjected to a direct slide-smearing for detection using a microscope and DNA sequencing, targeting the cytochrome b (Cyt b) gene of 10 polymerase chain reaction (PCR) products. The thin smear findings of the 200 suspected cases revealed that 63 (31.5%) were infected with <em>Theileria </em>spp., while 115 (57.5%) cases had no <em>Theileria </em>but other blood parasites; however, only 22 (11%) suspected cases showed no presence of any parasites. Unsurprisingly, the 25 blood samples from the control group demonstrated no presence of any blood parasite. Moreover, the DNA sequencing demonstrated that the <em>Theileria </em>spp. belonged to <em>T. annulata</em> species, and these sequences were nucleotide-based similar to Gene-Bank isolates from Tunisia (ON035604, ON035605, ON035606, ON035607, ON035608, ON035609, ON035610, ON035611, ON035612, and ON035613). The present study outcomes indicate that theileriosis is the dominant parasitic infection in cattle in Al-Hilla City and is highly caused by <em>Theileria annulata</em>. |
| first_indexed | 2024-04-09T20:10:34Z |
| format | Article |
| id | doaj.art-18b0f4781d314d1a9834105f23847ee5 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-09T20:10:34Z |
| publishDate | 2023-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-18b0f4781d314d1a9834105f23847ee52023-03-31T23:07:07ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552023-04-0137242542910.33899/ijvs.2022.135154.2450177329Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, IraqAyat Q. Jawad0Monyer A. Al-Fatlawi1Department of Veterinary Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, IraqDepartment of Veterinary Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, IraqThe current work was conducted to unveil the current situation for the infection by<em> Theileria annulata</em> in cattle in Al-Hilla City, Iraq. A total of 225 blood samples (200 from suspected infected animals and 25 from clinically healthy animals as a control group) were collected. These samples were subjected to a direct slide-smearing for detection using a microscope and DNA sequencing, targeting the cytochrome b (Cyt b) gene of 10 polymerase chain reaction (PCR) products. The thin smear findings of the 200 suspected cases revealed that 63 (31.5%) were infected with <em>Theileria </em>spp., while 115 (57.5%) cases had no <em>Theileria </em>but other blood parasites; however, only 22 (11%) suspected cases showed no presence of any parasites. Unsurprisingly, the 25 blood samples from the control group demonstrated no presence of any blood parasite. Moreover, the DNA sequencing demonstrated that the <em>Theileria </em>spp. belonged to <em>T. annulata</em> species, and these sequences were nucleotide-based similar to Gene-Bank isolates from Tunisia (ON035604, ON035605, ON035606, ON035607, ON035608, ON035609, ON035610, ON035611, ON035612, and ON035613). The present study outcomes indicate that theileriosis is the dominant parasitic infection in cattle in Al-Hilla City and is highly caused by <em>Theileria annulata</em>.https://www.vetmedmosul.com/article_177329_5570cea502f844ddc2ee34acc897dc5f.pdfgene sequencingparasitic infectiontheileria annulatatheileriosis |
| spellingShingle | Ayat Q. Jawad Monyer A. Al-Fatlawi Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, Iraq Iraqi Journal of Veterinary Sciences gene sequencing parasitic infection theileria annulata theileriosis |
| title | Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, Iraq |
| title_full | Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, Iraq |
| title_fullStr | Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, Iraq |
| title_full_unstemmed | Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, Iraq |
| title_short | Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, Iraq |
| title_sort | molecular study and dna sequence analysis of theileria annulata in cattle in al hilla iraq |
| topic | gene sequencing parasitic infection theileria annulata theileriosis |
| url | https://www.vetmedmosul.com/article_177329_5570cea502f844ddc2ee34acc897dc5f.pdf |
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