Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration
Small molecules are ubiquitous in nature and their detection is relevant in various domains. However, due to their size, sensitive and selective probes are difficult to select and the detection methods are generally indirect. In this study, we introduced the use of melting curve analysis of aptachai...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/4/112 |
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| author | Chenze Lu Christine Saint-Pierre Didier Gasparutto Yoann Roupioz Corinne Ravelet Eric Peyrin Arnaud Buhot |
| author_facet | Chenze Lu Christine Saint-Pierre Didier Gasparutto Yoann Roupioz Corinne Ravelet Eric Peyrin Arnaud Buhot |
| author_sort | Chenze Lu |
| collection | DOAJ |
| description | Small molecules are ubiquitous in nature and their detection is relevant in various domains. However, due to their size, sensitive and selective probes are difficult to select and the detection methods are generally indirect. In this study, we introduced the use of melting curve analysis of aptachains based on split-aptamers for the detection of adenosine. Aptamers, short oligonucleotides, are known to be particularly efficient probes compared to antibodies thanks to their advantageous probe/target size ratio. Aptachains are formed from dimers with dangling ends followed by the split-aptamer binding triggered by the presence of the target. The high melting temperature of the dimers served as a calibration for the detection/quantification of the target based on the height and/or temperature shift of the aptachain melting peak. |
| first_indexed | 2024-03-10T12:30:35Z |
| format | Article |
| id | doaj.art-18b3745d1ed3435580fb4c15f0ee56c2 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T12:30:35Z |
| publishDate | 2021-04-01 |
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| series | Biosensors |
| spelling | doaj.art-18b3745d1ed3435580fb4c15f0ee56c22023-11-21T14:41:16ZengMDPI AGBiosensors2079-63742021-04-0111411210.3390/bios11040112Melting Curve Analysis of Aptachains: Adenosine Detection with Internal CalibrationChenze Lu0Christine Saint-Pierre1Didier Gasparutto2Yoann Roupioz3Corinne Ravelet4Eric Peyrin5Arnaud Buhot6College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaUniversity Grenoble Alpes, CEA, CNRS, IRIG, SyMMES, F-38000 Grenoble, FranceUniversity Grenoble Alpes, CEA, CNRS, IRIG, SyMMES, F-38000 Grenoble, FranceUniversity Grenoble Alpes, CEA, CNRS, IRIG, SyMMES, F-38000 Grenoble, FranceUniversity Grenoble Alpes, CNRS, DPM, F-38000 Grenoble, FranceUniversity Grenoble Alpes, CNRS, DPM, F-38000 Grenoble, FranceUniversity Grenoble Alpes, CEA, CNRS, IRIG, SyMMES, F-38000 Grenoble, FranceSmall molecules are ubiquitous in nature and their detection is relevant in various domains. However, due to their size, sensitive and selective probes are difficult to select and the detection methods are generally indirect. In this study, we introduced the use of melting curve analysis of aptachains based on split-aptamers for the detection of adenosine. Aptamers, short oligonucleotides, are known to be particularly efficient probes compared to antibodies thanks to their advantageous probe/target size ratio. Aptachains are formed from dimers with dangling ends followed by the split-aptamer binding triggered by the presence of the target. The high melting temperature of the dimers served as a calibration for the detection/quantification of the target based on the height and/or temperature shift of the aptachain melting peak.https://www.mdpi.com/2079-6374/11/4/112split-aptamerssmall molecule detectionaptachain self-assemblymelting temperaturecalibration/normalization |
| spellingShingle | Chenze Lu Christine Saint-Pierre Didier Gasparutto Yoann Roupioz Corinne Ravelet Eric Peyrin Arnaud Buhot Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration Biosensors split-aptamers small molecule detection aptachain self-assembly melting temperature calibration/normalization |
| title | Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration |
| title_full | Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration |
| title_fullStr | Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration |
| title_full_unstemmed | Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration |
| title_short | Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration |
| title_sort | melting curve analysis of aptachains adenosine detection with internal calibration |
| topic | split-aptamers small molecule detection aptachain self-assembly melting temperature calibration/normalization |
| url | https://www.mdpi.com/2079-6374/11/4/112 |
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