Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells
Ethyl acetate fraction from Swietenia macrophylla was reported to have toxicity against the larvae of Artemia salina shrimp larvae. However, there areno reports aboutS. macrophylla, which can inhibit human breast cancer cells MCF-7. Therefore, this study aims to evaluate S. macrophyllaextract's...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
2020-07-01
|
| Series: | Jurnal Kimia Sains dan Aplikasi |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/ksa/article/view/27149 |
| _version_ | 1828132618705567744 |
|---|---|
| author | Dudi Tohir Fitriah Sari Irma Herawati Suparto |
| author_facet | Dudi Tohir Fitriah Sari Irma Herawati Suparto |
| author_sort | Dudi Tohir |
| collection | DOAJ |
| description | Ethyl acetate fraction from Swietenia macrophylla was reported to have toxicity against the larvae of Artemia salina shrimp larvae. However, there areno reports aboutS. macrophylla, which can inhibit human breast cancer cells MCF-7. Therefore, this study aims to evaluate S. macrophyllaextract's cytotoxicity using human breast cancer MCF-7 cells assay, followed by confirmation of its toxicity using brine shrimp lethality assay. The most active fraction obtained from the ethyl acetate extract of S. macrophylla showed 76.49% inhibition at 50 µg/mL (IC50=34.11 µg/mL). At the same time, the most active fraction may contain a mixture of limonoid compounds after LCMS analysis. The most active fraction obtained from ethyl acetate extract of S. macrophylla showed 76.49% |
| first_indexed | 2024-04-11T17:07:43Z |
| format | Article |
| id | doaj.art-18b46050bae747c4ade8a97ec51efc2e |
| institution | Directory Open Access Journal |
| issn | 1410-8917 2597-9914 |
| language | English |
| last_indexed | 2024-04-11T17:07:43Z |
| publishDate | 2020-07-01 |
| publisher | Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University |
| record_format | Article |
| series | Jurnal Kimia Sains dan Aplikasi |
| spelling | doaj.art-18b46050bae747c4ade8a97ec51efc2e2022-12-22T04:12:59ZengChemistry Department, Faculty of Sciences and Mathematics, Diponegoro UniversityJurnal Kimia Sains dan Aplikasi1410-89172597-99142020-07-0123723423710.14710/jksa.23.7.234-23717088Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 CellsDudi Tohir0Fitriah Sari1Irma Herawati Suparto2https://orcid.org/0000-0003-1682-6467Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, IPB University, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, IPB University, IndonesiaEthyl acetate fraction from Swietenia macrophylla was reported to have toxicity against the larvae of Artemia salina shrimp larvae. However, there areno reports aboutS. macrophylla, which can inhibit human breast cancer cells MCF-7. Therefore, this study aims to evaluate S. macrophyllaextract's cytotoxicity using human breast cancer MCF-7 cells assay, followed by confirmation of its toxicity using brine shrimp lethality assay. The most active fraction obtained from the ethyl acetate extract of S. macrophylla showed 76.49% inhibition at 50 µg/mL (IC50=34.11 µg/mL). At the same time, the most active fraction may contain a mixture of limonoid compounds after LCMS analysis. The most active fraction obtained from ethyl acetate extract of S. macrophylla showed 76.49%https://ejournal.undip.ac.id/index.php/ksa/article/view/27149cytotoxicitymcf-7 cellsswietenia macrophyllalc-ms |
| spellingShingle | Dudi Tohir Fitriah Sari Irma Herawati Suparto Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells Jurnal Kimia Sains dan Aplikasi cytotoxicity mcf-7 cells swietenia macrophylla lc-ms |
| title | Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells |
| title_full | Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells |
| title_fullStr | Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells |
| title_full_unstemmed | Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells |
| title_short | Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells |
| title_sort | cytotoxicity of the most active fraction of the seeds of swietenia macrophylla using human breast cancer mcf 7 cells |
| topic | cytotoxicity mcf-7 cells swietenia macrophylla lc-ms |
| url | https://ejournal.undip.ac.id/index.php/ksa/article/view/27149 |
| work_keys_str_mv | AT duditohir cytotoxicityofthemostactivefractionoftheseedsofswieteniamacrophyllausinghumanbreastcancermcf7cells AT fitriahsari cytotoxicityofthemostactivefractionoftheseedsofswieteniamacrophyllausinghumanbreastcancermcf7cells AT irmaherawatisuparto cytotoxicityofthemostactivefractionoftheseedsofswieteniamacrophyllausinghumanbreastcancermcf7cells |