Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs
Abstract Brain ethanolamine plasmalogens (EPls) are the only known ligands of G-protein-coupled receptor 61, a novel receptor that stimulates follicle-stimulating hormone (FSH), but not luteinizing hormone (LH), secretion by bovine gonadotrophs. We hypothesized that the recently developed neuroprote...
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| Format: | Article |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20794-4 |
| _version_ | 1811194812023439360 |
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| author | Hiroya Kadokawa Miyako Kotaniguchi Shiro Mawatari Risa Saito Takehiko Fujino Shinichi Kitamura |
| author_facet | Hiroya Kadokawa Miyako Kotaniguchi Shiro Mawatari Risa Saito Takehiko Fujino Shinichi Kitamura |
| author_sort | Hiroya Kadokawa |
| collection | DOAJ |
| description | Abstract Brain ethanolamine plasmalogens (EPls) are the only known ligands of G-protein-coupled receptor 61, a novel receptor that stimulates follicle-stimulating hormone (FSH), but not luteinizing hormone (LH), secretion by bovine gonadotrophs. We hypothesized that the recently developed neuroprotective EPls extracted from scallop (Pecten yessoensis) (scallop EPls) could stimulate FSH secretion by gonadotrophs. To test this hypothesis, bovine gonadotrophs were cultured for 3.5 days and treated with increasing concentrations of scallop EPls. FSH secretion was stimulated by all tested concentrations of scallop EPls (P < 0.05). Surprisingly, LH secretion was stimulated by both 0.5 (P < 0.05) and 5 (P < 0.01) ng/mL of scallop EPls. To clarify the important differences between bovine brain and scallop EPls, we utilized two-dimensional liquid chromatography–mass spectrometry, which revealed 44 peaks, including 10 large peaks. Among them, eight were scallop-specific EPl molecular species, occupying approximately 58% of the total area percentage of scallop EPls. Almost all large peaks contained 4, 5, or 6 unsaturated double bonds in the carbon chain at the sn-2 position of the glycerol backbone. Our results showed that EPls from scallops, lacking pituitary glands, stimulated both FSH and LH secretion by bovine gonadotrophs. |
| first_indexed | 2024-04-12T00:32:46Z |
| format | Article |
| id | doaj.art-18b59587ee2e497bbdd9b54789cc0f77 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T00:32:46Z |
| publishDate | 2022-10-01 |
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| series | Scientific Reports |
| spelling | doaj.art-18b59587ee2e497bbdd9b54789cc0f772022-12-22T03:55:16ZengNature PortfolioScientific Reports2045-23222022-10-0112111210.1038/s41598-022-20794-4Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophsHiroya Kadokawa0Miyako Kotaniguchi1Shiro Mawatari2Risa Saito3Takehiko Fujino4Shinichi Kitamura5Faculty of Veterinary Medicine, Yamaguchi UniversityInternational Polysaccharide Engineering (IPE) Inc., Laboratory of Advanced Food Process Engineering, Organization for Research Promotion, Osaka Metropolitan UniversityInstitute of Rheological Functions of FoodFaculty of Veterinary Medicine, Yamaguchi UniversityInstitute of Rheological Functions of FoodLaboratory of Advanced Food Process Engineering, Organization for Research Promotion, Osaka Metropolitan, UniversityAbstract Brain ethanolamine plasmalogens (EPls) are the only known ligands of G-protein-coupled receptor 61, a novel receptor that stimulates follicle-stimulating hormone (FSH), but not luteinizing hormone (LH), secretion by bovine gonadotrophs. We hypothesized that the recently developed neuroprotective EPls extracted from scallop (Pecten yessoensis) (scallop EPls) could stimulate FSH secretion by gonadotrophs. To test this hypothesis, bovine gonadotrophs were cultured for 3.5 days and treated with increasing concentrations of scallop EPls. FSH secretion was stimulated by all tested concentrations of scallop EPls (P < 0.05). Surprisingly, LH secretion was stimulated by both 0.5 (P < 0.05) and 5 (P < 0.01) ng/mL of scallop EPls. To clarify the important differences between bovine brain and scallop EPls, we utilized two-dimensional liquid chromatography–mass spectrometry, which revealed 44 peaks, including 10 large peaks. Among them, eight were scallop-specific EPl molecular species, occupying approximately 58% of the total area percentage of scallop EPls. Almost all large peaks contained 4, 5, or 6 unsaturated double bonds in the carbon chain at the sn-2 position of the glycerol backbone. Our results showed that EPls from scallops, lacking pituitary glands, stimulated both FSH and LH secretion by bovine gonadotrophs.https://doi.org/10.1038/s41598-022-20794-4 |
| spellingShingle | Hiroya Kadokawa Miyako Kotaniguchi Shiro Mawatari Risa Saito Takehiko Fujino Shinichi Kitamura Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs Scientific Reports |
| title | Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs |
| title_full | Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs |
| title_fullStr | Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs |
| title_full_unstemmed | Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs |
| title_short | Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs |
| title_sort | ethanolamine plasmalogens derived from scallops stimulate both follicle stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs |
| url | https://doi.org/10.1038/s41598-022-20794-4 |
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