Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation
Plant phytochemicals are described as possessing considerable neuroprotective properties, due to radical scavenging capacity and acetylcholinesterase inhibitory activity, important bioactivities in neurodegeneration. Antirrhinum lopesianum is a rare endemism from the Iberian Peninsula, occurring at...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-10-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2076-3921/2/4/273 |
| _version_ | 1797762572072517632 |
|---|---|
| author | Andreia Gomes Sofia Fortalezas Rui Pimpão Inês Figueira João Maroco Carlos Aguiar Ricardo B. Ferreira Célia Miguel Cláudia N. Santos |
| author_facet | Andreia Gomes Sofia Fortalezas Rui Pimpão Inês Figueira João Maroco Carlos Aguiar Ricardo B. Ferreira Célia Miguel Cláudia N. Santos |
| author_sort | Andreia Gomes |
| collection | DOAJ |
| description | Plant phytochemicals are described as possessing considerable neuroprotective properties, due to radical scavenging capacity and acetylcholinesterase inhibitory activity, important bioactivities in neurodegeneration. Antirrhinum lopesianum is a rare endemism from the Iberian Peninsula, occurring at the northeastern border between Portugal and Spain. It is classified as Endangered, due to its highly fragmented geographical occupation, facing a high risk of extinction in the Portuguese territory, within 20 years. Here, we describe for the first time the chemical characterization of extracts of the species concerning total phenol content, flavonoid content and antioxidant properties. The profile of high performance liquid chromatography with diode array detector (HPLC-DAD) of the polyphenol-enriched fraction of plant extracts was also performed, showing the great potential of the species as a source of bioactive phytochemical compounds. A. lopesianum’s potential for neuroprotection was revealed by a significant acetylcholinesterase inhibitory activity and also by a neuroprotective effect on a human cell model of neurodegeneration. Moreover, this is the first report describing a successful procedure for the in vitro propagation of this endangered species. The comparison of phenolic content and the HPLC-DAD profile of wild and in vitro propagated plants revealed that in vitro plants maintain the ability to produce secondary metabolites, but the profiles are differentially affected by the growth regulators. The results presented here greatly contribute to the value for this species regarding its potential as a source of phytochemicals with prospective neuroprotective health benefits. |
| first_indexed | 2024-03-12T19:30:16Z |
| format | Article |
| id | doaj.art-18b9323955ee48a0a7914167f1d61f9b |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T19:30:16Z |
| publishDate | 2013-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-18b9323955ee48a0a7914167f1d61f9b2023-08-02T04:36:39ZengMDPI AGAntioxidants2076-39212013-10-012427329210.3390/antiox2040273Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant PropagationAndreia GomesSofia FortalezasRui PimpãoInês FigueiraJoão MarocoCarlos AguiarRicardo B. FerreiraCélia MiguelCláudia N. SantosPlant phytochemicals are described as possessing considerable neuroprotective properties, due to radical scavenging capacity and acetylcholinesterase inhibitory activity, important bioactivities in neurodegeneration. Antirrhinum lopesianum is a rare endemism from the Iberian Peninsula, occurring at the northeastern border between Portugal and Spain. It is classified as Endangered, due to its highly fragmented geographical occupation, facing a high risk of extinction in the Portuguese territory, within 20 years. Here, we describe for the first time the chemical characterization of extracts of the species concerning total phenol content, flavonoid content and antioxidant properties. The profile of high performance liquid chromatography with diode array detector (HPLC-DAD) of the polyphenol-enriched fraction of plant extracts was also performed, showing the great potential of the species as a source of bioactive phytochemical compounds. A. lopesianum’s potential for neuroprotection was revealed by a significant acetylcholinesterase inhibitory activity and also by a neuroprotective effect on a human cell model of neurodegeneration. Moreover, this is the first report describing a successful procedure for the in vitro propagation of this endangered species. The comparison of phenolic content and the HPLC-DAD profile of wild and in vitro propagated plants revealed that in vitro plants maintain the ability to produce secondary metabolites, but the profiles are differentially affected by the growth regulators. The results presented here greatly contribute to the value for this species regarding its potential as a source of phytochemicals with prospective neuroprotective health benefits.http://www.mdpi.com/2076-3921/2/4/273Antirrhinum lopesianumphytochemical profileantioxidant capacityneuroprotectionacetylcholinesterase inhibitorex situ conservation |
| spellingShingle | Andreia Gomes Sofia Fortalezas Rui Pimpão Inês Figueira João Maroco Carlos Aguiar Ricardo B. Ferreira Célia Miguel Cláudia N. Santos Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation Antioxidants Antirrhinum lopesianum phytochemical profile antioxidant capacity neuroprotection acetylcholinesterase inhibitor ex situ conservation |
| title | Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation |
| title_full | Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation |
| title_fullStr | Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation |
| title_full_unstemmed | Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation |
| title_short | Valuing the Endangered Species Antirrhinum lopesianum: Neuroprotective Activities and Strategies for in vitro Plant Propagation |
| title_sort | valuing the endangered species antirrhinum lopesianum neuroprotective activities and strategies for in vitro plant propagation |
| topic | Antirrhinum lopesianum phytochemical profile antioxidant capacity neuroprotection acetylcholinesterase inhibitor ex situ conservation |
| url | http://www.mdpi.com/2076-3921/2/4/273 |
| work_keys_str_mv | AT andreiagomes valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation AT sofiafortalezas valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation AT ruipimpao valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation AT inesfigueira valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation AT joaomaroco valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation AT carlosaguiar valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation AT ricardobferreira valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation AT celiamiguel valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation AT claudiansantos valuingtheendangeredspeciesantirrhinumlopesianumneuroprotectiveactivitiesandstrategiesforinvitroplantpropagation |