Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development
The technique of cloning has wide applications in animal husbandry and human biomedicine. However, the very low developmental efficiency of cloned embryos limits the application of cloning. Ectopic <i>XIST</i>-expression-induced abnormal X chromosome inactivation (XCI) is a primary cause...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/14572 |
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| author | Yazheng Dong Xiao Wu Xitong Peng Liusong Yang Baohua Tan Huaxing Zhao Enqin Zheng Linjun Hong Gengyuan Cai Zhenfang Wu Zicong Li |
| author_facet | Yazheng Dong Xiao Wu Xitong Peng Liusong Yang Baohua Tan Huaxing Zhao Enqin Zheng Linjun Hong Gengyuan Cai Zhenfang Wu Zicong Li |
| author_sort | Yazheng Dong |
| collection | DOAJ |
| description | The technique of cloning has wide applications in animal husbandry and human biomedicine. However, the very low developmental efficiency of cloned embryos limits the application of cloning. Ectopic <i>XIST</i>-expression-induced abnormal X chromosome inactivation (XCI) is a primary cause of the low developmental competence of cloned mouse and pig embryos. Knockout or knockdown of <i>XIST</i> improves cloning efficiency in both pigs and mice. The transcription factor Yin yang 1(YY1) plays a critical role in XCI by triggering the transcription of X-inactive specific transcript <i>(XIST)</i> and facilitating the localization of <i>XIST</i> RNA on the X chromosome. This study aimed to investigate whether RNA interference to suppress the expression of YY1 can inhibit erroneous <i>XIST</i> expression, rescue abnormal XCI, and improve the developmental ability of cloned pig embryos. The results showed that YY1 binds to the 5′ regulatory region of the porcine <i>XIST</i> gene in pig cells. The microinjection of YY1 siRNA into cloned pig embryos reduced the transcript abundance of <i>XIST</i> and upregulated the mRNA level of X-linked genes at the 4-cell and blastocyst stages. The siRNA-mediated knockdown of YY1 altered the transcriptome and enhanced the in vitro and in vivo developmental efficiency of cloned porcine embryos. These results suggested that YY1 participates in regulating <i>XIST</i> expression and XCI in cloned pig embryos and that the suppression of YY1 expression can increase the developmental rate of cloned pig embryos. The present study established a new method for improving the efficiency of pig cloning. |
| first_indexed | 2024-03-09T17:47:10Z |
| format | Article |
| id | doaj.art-18b957ba56584551836f2396e7e1b701 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:47:10Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-18b957ba56584551836f2396e7e1b7012023-11-24T11:04:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231457210.3390/ijms232314572Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo DevelopmentYazheng Dong0Xiao Wu1Xitong Peng2Liusong Yang3Baohua Tan4Huaxing Zhao5Enqin Zheng6Linjun Hong7Gengyuan Cai8Zhenfang Wu9Zicong Li10National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510030, ChinaThe technique of cloning has wide applications in animal husbandry and human biomedicine. However, the very low developmental efficiency of cloned embryos limits the application of cloning. Ectopic <i>XIST</i>-expression-induced abnormal X chromosome inactivation (XCI) is a primary cause of the low developmental competence of cloned mouse and pig embryos. Knockout or knockdown of <i>XIST</i> improves cloning efficiency in both pigs and mice. The transcription factor Yin yang 1(YY1) plays a critical role in XCI by triggering the transcription of X-inactive specific transcript <i>(XIST)</i> and facilitating the localization of <i>XIST</i> RNA on the X chromosome. This study aimed to investigate whether RNA interference to suppress the expression of YY1 can inhibit erroneous <i>XIST</i> expression, rescue abnormal XCI, and improve the developmental ability of cloned pig embryos. The results showed that YY1 binds to the 5′ regulatory region of the porcine <i>XIST</i> gene in pig cells. The microinjection of YY1 siRNA into cloned pig embryos reduced the transcript abundance of <i>XIST</i> and upregulated the mRNA level of X-linked genes at the 4-cell and blastocyst stages. The siRNA-mediated knockdown of YY1 altered the transcriptome and enhanced the in vitro and in vivo developmental efficiency of cloned porcine embryos. These results suggested that YY1 participates in regulating <i>XIST</i> expression and XCI in cloned pig embryos and that the suppression of YY1 expression can increase the developmental rate of cloned pig embryos. The present study established a new method for improving the efficiency of pig cloning.https://www.mdpi.com/1422-0067/23/23/14572cloned pig embryosX chromosome inactivationYY1<i>XIST</i> |
| spellingShingle | Yazheng Dong Xiao Wu Xitong Peng Liusong Yang Baohua Tan Huaxing Zhao Enqin Zheng Linjun Hong Gengyuan Cai Zhenfang Wu Zicong Li Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development International Journal of Molecular Sciences cloned pig embryos X chromosome inactivation YY1 <i>XIST</i> |
| title | Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development |
| title_full | Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development |
| title_fullStr | Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development |
| title_full_unstemmed | Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development |
| title_short | Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development |
| title_sort | knockdown of yy1 inhibits i xist i expression and enhances cloned pig embryo development |
| topic | cloned pig embryos X chromosome inactivation YY1 <i>XIST</i> |
| url | https://www.mdpi.com/1422-0067/23/23/14572 |
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