Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer
Harnessing the potential of cells as complex biosensors promises the potential to create sensitive and selective detectors for discrimination of biodefense agents. Here we present toxin detection and suggest discrimination using cells in a multianalyte microphysiometer (MMP) that is capable of simul...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2009-03-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/9/3/2117/ |
| _version_ | 1811185257731325952 |
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| author | John Wikswo David E. Cliffel David W. Wright Clare K. Carney Roy G. Thompson Rachel M. Snider Sven E. Eklund |
| author_facet | John Wikswo David E. Cliffel David W. Wright Clare K. Carney Roy G. Thompson Rachel M. Snider Sven E. Eklund |
| author_sort | John Wikswo |
| collection | DOAJ |
| description | Harnessing the potential of cells as complex biosensors promises the potential to create sensitive and selective detectors for discrimination of biodefense agents. Here we present toxin detection and suggest discrimination using cells in a multianalyte microphysiometer (MMP) that is capable of simultaneously measuring flux changes in four extracellular analytes (acidification rate, glucose uptake, oxygen uptake, and lactate production) in real-time. Differential short-term cellular responses were observed between botulinum neurotoxin A and ricin toxin with neuroblastoma cells, alamethicin and anthrax protective antigen with RAW macrophages, and cholera toxin, muscarine, 2,4-dinitro-phenol, and NaF with CHO cells. These results and the post exposure dynamics and metabolic recovery observed in each case suggest the usefulness of cell-based detectors to discriminate between specific analytes and classes of compounds in a complex matrix, and furthermore to make metabolic inferences on the cellular effects of the agents. This may be particularly valuable for classifying unknown toxins. |
| first_indexed | 2024-04-11T13:27:28Z |
| format | Article |
| id | doaj.art-18bc5dbb615d45d2a20b75e558ee91e2 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:27:28Z |
| publishDate | 2009-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-18bc5dbb615d45d2a20b75e558ee91e22022-12-22T04:22:00ZengMDPI AGSensors1424-82202009-03-01932117213310.3390/s90302117Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte MicrophysiometerJohn WikswoDavid E. CliffelDavid W. WrightClare K. CarneyRoy G. ThompsonRachel M. SniderSven E. EklundHarnessing the potential of cells as complex biosensors promises the potential to create sensitive and selective detectors for discrimination of biodefense agents. Here we present toxin detection and suggest discrimination using cells in a multianalyte microphysiometer (MMP) that is capable of simultaneously measuring flux changes in four extracellular analytes (acidification rate, glucose uptake, oxygen uptake, and lactate production) in real-time. Differential short-term cellular responses were observed between botulinum neurotoxin A and ricin toxin with neuroblastoma cells, alamethicin and anthrax protective antigen with RAW macrophages, and cholera toxin, muscarine, 2,4-dinitro-phenol, and NaF with CHO cells. These results and the post exposure dynamics and metabolic recovery observed in each case suggest the usefulness of cell-based detectors to discriminate between specific analytes and classes of compounds in a complex matrix, and furthermore to make metabolic inferences on the cellular effects of the agents. This may be particularly valuable for classifying unknown toxins.http://www.mdpi.com/1424-8220/9/3/2117/toxinbiotoxinbiosensorsmicrophysiometry |
| spellingShingle | John Wikswo David E. Cliffel David W. Wright Clare K. Carney Roy G. Thompson Rachel M. Snider Sven E. Eklund Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer Sensors toxin biotoxin biosensors microphysiometry |
| title | Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer |
| title_full | Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer |
| title_fullStr | Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer |
| title_full_unstemmed | Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer |
| title_short | Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer |
| title_sort | metabolic discrimination of select list agents by monitoring cellular responses in a multianalyte microphysiometer |
| topic | toxin biotoxin biosensors microphysiometry |
| url | http://www.mdpi.com/1424-8220/9/3/2117/ |
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