NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis
The periplasmic nitrate reductase enzyme (NAP) has become attractive catalyst, whose exploitation has emerged as one of the indispensable strategies toward environmentally benign applications. To achieve them efficiently and overcome the sensitivity of NAP in harsh environmental circumstances, the i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-06-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X17303545 |
| _version_ | 1811194257160011776 |
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| author | Marwa Eltarahony Sahar Zaki Zeinab Kheiralla Desouky Abd-El-haleem |
| author_facet | Marwa Eltarahony Sahar Zaki Zeinab Kheiralla Desouky Abd-El-haleem |
| author_sort | Marwa Eltarahony |
| collection | DOAJ |
| description | The periplasmic nitrate reductase enzyme (NAP) has become attractive catalyst, whose exploitation has emerged as one of the indispensable strategies toward environmentally benign applications. To achieve them efficiently and overcome the sensitivity of NAP in harsh environmental circumstances, the immobilization for denitrifying bacteria and NAP enzyme for simultaneous bioremediation and bionanoparticles synthesis was studied. NAP catalyzed NO3− reduction at Vmax of 0.811 μM/min and Km of 14.02 mM. Concurrently, the immobilized MMT cells completely removed NO3- upon 192 h with AgNPs synthesis ranging from 23.26 to 58.14 nm as indicated by SEM. Wherase, immobilized NAP exhibited lower efficiency with 28.6% of NO3− elimination within 288 h and large aggregated AgNPs ranging from 94.44 nm to 172.22 nm. To the best of author knowledge, the immobilization for denitrifying bacteria and NAP enzyme for simultaneous bioremediation and bionanoparticles synthesis was not studied before. |
| first_indexed | 2024-04-12T00:23:01Z |
| format | Article |
| id | doaj.art-18be3a4218d9401783c8a6968fd02c52 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-12T00:23:01Z |
| publishDate | 2018-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-18be3a4218d9401783c8a6968fd02c522022-12-22T03:55:36ZengElsevierBiotechnology Reports2215-017X2018-06-0118C10.1016/j.btre.2018.e00257NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesisMarwa Eltarahony0Sahar Zaki1Zeinab Kheiralla2Desouky Abd-El-haleem3Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, 21934 Borgelarab, Alexandria, EgyptEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, 21934 Borgelarab, Alexandria, EgyptBotany Department, College of Women for Arts, Science and Education, Ain Shams University, Cairo, EgyptEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, 21934 Borgelarab, Alexandria, EgyptThe periplasmic nitrate reductase enzyme (NAP) has become attractive catalyst, whose exploitation has emerged as one of the indispensable strategies toward environmentally benign applications. To achieve them efficiently and overcome the sensitivity of NAP in harsh environmental circumstances, the immobilization for denitrifying bacteria and NAP enzyme for simultaneous bioremediation and bionanoparticles synthesis was studied. NAP catalyzed NO3− reduction at Vmax of 0.811 μM/min and Km of 14.02 mM. Concurrently, the immobilized MMT cells completely removed NO3- upon 192 h with AgNPs synthesis ranging from 23.26 to 58.14 nm as indicated by SEM. Wherase, immobilized NAP exhibited lower efficiency with 28.6% of NO3− elimination within 288 h and large aggregated AgNPs ranging from 94.44 nm to 172.22 nm. To the best of author knowledge, the immobilization for denitrifying bacteria and NAP enzyme for simultaneous bioremediation and bionanoparticles synthesis was not studied before.http://www.sciencedirect.com/science/article/pii/S2215017X17303545ImmobilizationNitrate reductaseNanoparticlesDenitrificationBioremediation |
| spellingShingle | Marwa Eltarahony Sahar Zaki Zeinab Kheiralla Desouky Abd-El-haleem NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis Biotechnology Reports Immobilization Nitrate reductase Nanoparticles Denitrification Bioremediation |
| title | NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis |
| title_full | NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis |
| title_fullStr | NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis |
| title_full_unstemmed | NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis |
| title_short | NAP enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis |
| title_sort | nap enzyme recruitment in simultaneous bioremediation and nanoparticles synthesis |
| topic | Immobilization Nitrate reductase Nanoparticles Denitrification Bioremediation |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X17303545 |
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