Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro
Fasciola gigantica is a parasite that causes a disruption of the metabolism of fats, proteins, and carbohydrates, which interferes growth and causes death. Curcuma aeruginosa Roxb extract is one of the medicinal plants which has been used to treat several diseases. The aim of this study was to deter...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2020-01-01
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| Series: | E3S Web of Conferences |
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| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01046.pdf |
| _version_ | 1818411652086759424 |
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| author | Vanda Henni Parindra Rizki Hambal Muhammad Athaillah Farida |
| author_facet | Vanda Henni Parindra Rizki Hambal Muhammad Athaillah Farida |
| author_sort | Vanda Henni |
| collection | DOAJ |
| description | Fasciola gigantica is a parasite that causes a disruption of the metabolism of fats, proteins, and carbohydrates, which interferes growth and causes death. Curcuma aeruginosa Roxb extract is one of the medicinal plants which has been used to treat several diseases. The aim of this study was to determine the effect of methanolic extract of C. aeruginosa Roxb on F. gigantica, including mortality time and histopathological changes that occurred after treatment. This study used a completely randomized design with five replications. The flukes were soaked in three different extract concentrations: 10% (T1), 25% (T2), and 50% (T3) (w/v). Phosphate buffered saline (PBS) solution was used as a negative control (C1) and albendazole as the positive control (C2). The mortality time of F. gigantica in each group was calculated, and the dead flukes were prepared for histopathological study. The data were analyzed by Analysis of variance. The results showed that C. aeruginosa extracts at the concentration of 10, 25 and 50% caused the death of the flukes within 75, 57 and 48 minutes, respectively., Histopathological observations showed that the extract caused breakage of tegument which is an important organ in the respiratory process and nutrient absorption. This study concluded that C. aeruginosa extract exhibited anthelmintic activity towards F. gigantica in vitro. |
| first_indexed | 2024-12-14T10:34:49Z |
| format | Article |
| id | doaj.art-18beb9a106e04d0fac5127551a2dcd32 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-14T10:34:49Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-18beb9a106e04d0fac5127551a2dcd322022-12-21T23:05:58ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011510104610.1051/e3sconf/202015101046e3sconf_icvaes2020_01046Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in VitroVanda Henni0Parindra Rizki1Hambal Muhammad2Athaillah Farida3Laboratory of Pharmacology , Faculty of Veterinary Medicine, Universitas Syiah KualaStudy Program of Veterinary Education, Faculty of Veterinary Medicine, Universitas Syiah KualaLaboratory of Parasitology , Faculty of Veterinary Medicine, Universitas Syiah KualaLaboratory of Parasitology , Faculty of Veterinary Medicine, Universitas Syiah KualaFasciola gigantica is a parasite that causes a disruption of the metabolism of fats, proteins, and carbohydrates, which interferes growth and causes death. Curcuma aeruginosa Roxb extract is one of the medicinal plants which has been used to treat several diseases. The aim of this study was to determine the effect of methanolic extract of C. aeruginosa Roxb on F. gigantica, including mortality time and histopathological changes that occurred after treatment. This study used a completely randomized design with five replications. The flukes were soaked in three different extract concentrations: 10% (T1), 25% (T2), and 50% (T3) (w/v). Phosphate buffered saline (PBS) solution was used as a negative control (C1) and albendazole as the positive control (C2). The mortality time of F. gigantica in each group was calculated, and the dead flukes were prepared for histopathological study. The data were analyzed by Analysis of variance. The results showed that C. aeruginosa extracts at the concentration of 10, 25 and 50% caused the death of the flukes within 75, 57 and 48 minutes, respectively., Histopathological observations showed that the extract caused breakage of tegument which is an important organ in the respiratory process and nutrient absorption. This study concluded that C. aeruginosa extract exhibited anthelmintic activity towards F. gigantica in vitro.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01046.pdffasciola giganticacurcuma aeruginosa roxb extractalbendazolepathological changes |
| spellingShingle | Vanda Henni Parindra Rizki Hambal Muhammad Athaillah Farida Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro E3S Web of Conferences fasciola gigantica curcuma aeruginosa roxb extract albendazole pathological changes |
| title | Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro |
| title_full | Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro |
| title_fullStr | Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro |
| title_full_unstemmed | Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro |
| title_short | Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro |
| title_sort | anthelmintic activity of curcuma aeruginosa roxb extract on fasciola gigantica in vitro |
| topic | fasciola gigantica curcuma aeruginosa roxb extract albendazole pathological changes |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01046.pdf |
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