Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study [version 2; peer review: 2 approved]
Background: Azo compounds, containing naphthol and diazonium salts, are synthetic dyes widely used in the batik industry. Azo compounds are considered toxic when they are exposed to human tissue. The purpose of this study was to analyze buccal cell DNA exposed to azo compounds in batik workers. Meth...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2020-09-01
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| Series: | F1000Research |
| Online Access: | https://f1000research.com/articles/9-1053/v2 |
| _version_ | 1818341772713000960 |
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| author | Juni Handajani Urfa Tabtila Nadia Rully Auliawati Abdul Rohman |
| author_facet | Juni Handajani Urfa Tabtila Nadia Rully Auliawati Abdul Rohman |
| author_sort | Juni Handajani |
| collection | DOAJ |
| description | Background: Azo compounds, containing naphthol and diazonium salts, are synthetic dyes widely used in the batik industry. Azo compounds are considered toxic when they are exposed to human tissue. The purpose of this study was to analyze buccal cell DNA exposed to azo compounds in batik workers. Methods: A cross-sectional study involving 20 male subjects divided into two groups (n=10 group), namely azo-exposed and non-exposed (control group). Inclusion criteria were batik workers of the colouring division who have been exposed to azo for at least 5 years. Buccal cells were taken using cytobrush then DNA were isolated from buccal cell. DNA isolation was done by buccal DNA kit, while the purity and concentration of the DNA was determined using spectrophotometer and electrophoresis. Results: The azo-exposed group revealed higher purity DNA than those in the control group. The purity of the DNA in the azo-exposed group and control group was 0.61±0.93 and 0.21±0.09, respectively, while the concentration of DNA was of 59.02 and 19.35 ng/UL, respectively. The ratio at 260/280 nm was 1.84-1.94 (azo-exposed) and 1.85-1.92 (control). Principal component analysis using the first principle component (PC1) and second principle component (PC2) could successfully classify subjects in the control and azo-exposed groups. Conclusion: Characteristics of DNA could be used as an indication of exposure to azo compounds in workers of batik industries. |
| first_indexed | 2024-12-13T16:04:07Z |
| format | Article |
| id | doaj.art-18becdb2157a456183f6813321240ab9 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-13T16:04:07Z |
| publishDate | 2020-09-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-18becdb2157a456183f6813321240ab92022-12-21T23:39:05ZengF1000 Research LtdF1000Research2046-14022020-09-01910.12688/f1000research.25798.229563Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study [version 2; peer review: 2 approved]Juni Handajani0Urfa Tabtila1Nadia Rully Auliawati2Abdul Rohman3Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, 55281, IndonesiaDental Study Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, 55281, IndonesiaDental Study Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, 55281, IndonesiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281, IndonesiaBackground: Azo compounds, containing naphthol and diazonium salts, are synthetic dyes widely used in the batik industry. Azo compounds are considered toxic when they are exposed to human tissue. The purpose of this study was to analyze buccal cell DNA exposed to azo compounds in batik workers. Methods: A cross-sectional study involving 20 male subjects divided into two groups (n=10 group), namely azo-exposed and non-exposed (control group). Inclusion criteria were batik workers of the colouring division who have been exposed to azo for at least 5 years. Buccal cells were taken using cytobrush then DNA were isolated from buccal cell. DNA isolation was done by buccal DNA kit, while the purity and concentration of the DNA was determined using spectrophotometer and electrophoresis. Results: The azo-exposed group revealed higher purity DNA than those in the control group. The purity of the DNA in the azo-exposed group and control group was 0.61±0.93 and 0.21±0.09, respectively, while the concentration of DNA was of 59.02 and 19.35 ng/UL, respectively. The ratio at 260/280 nm was 1.84-1.94 (azo-exposed) and 1.85-1.92 (control). Principal component analysis using the first principle component (PC1) and second principle component (PC2) could successfully classify subjects in the control and azo-exposed groups. Conclusion: Characteristics of DNA could be used as an indication of exposure to azo compounds in workers of batik industries.https://f1000research.com/articles/9-1053/v2 |
| spellingShingle | Juni Handajani Urfa Tabtila Nadia Rully Auliawati Abdul Rohman Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study [version 2; peer review: 2 approved] F1000Research |
| title | Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study [version 2; peer review: 2 approved] |
| title_full | Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study [version 2; peer review: 2 approved] |
| title_fullStr | Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study [version 2; peer review: 2 approved] |
| title_full_unstemmed | Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study [version 2; peer review: 2 approved] |
| title_short | Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study [version 2; peer review: 2 approved] |
| title_sort | characterization of buccal cell dna after exposure to azo compounds a cross sectional study version 2 peer review 2 approved |
| url | https://f1000research.com/articles/9-1053/v2 |
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