Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic.
<h4>Purpose</h4>Whole genome sequencing of SARS-CoV2 is important to find useful information about the viral lineages, variants of interests and variants of concern. As there are not enough data about the circulating SARS-CoV2 variants in Iran, we sequenced 54 SARS-CoV2 genomes during th...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0267847 |
| _version_ | 1818195836032516096 |
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| author | Jila Yavarian Ahmad Nejati Vahid Salimi Nazanin Zahra Shafiei Jandaghi Kaveh Sadeghi Adel Abedi Ali Sharifi Zarchi Mohammad Mehdi Gouya Talat Mokhtari-Azad |
| author_facet | Jila Yavarian Ahmad Nejati Vahid Salimi Nazanin Zahra Shafiei Jandaghi Kaveh Sadeghi Adel Abedi Ali Sharifi Zarchi Mohammad Mehdi Gouya Talat Mokhtari-Azad |
| author_sort | Jila Yavarian |
| collection | DOAJ |
| description | <h4>Purpose</h4>Whole genome sequencing of SARS-CoV2 is important to find useful information about the viral lineages, variants of interests and variants of concern. As there are not enough data about the circulating SARS-CoV2 variants in Iran, we sequenced 54 SARS-CoV2 genomes during the 5 waves of pandemic in Iran.<h4>Methods</h4>After viral RNA extraction from clinical samples collected during the COVID-19 pandemic, next generation sequencing was performed using the Nextseq platform. The sequencing data were analyzed and compared with reference sequences.<h4>Results</h4>During the 1st wave, V and L clades were detected. The second wave was recognized by G, GH and GR clades. Circulating clades during the 3rd wave were GH and GR. In the fourth wave GRY (alpha variant), GK (delta variant) and one GH clade (beta variant) were detected. All viruses in the fifth wave were in clade GK (delta variant). There were different mutations in all parts of the genomes but Spike-D614G, NSP12-P323L, N-R203K and N-G204R were the most frequent mutants in these studied viruses.<h4>Conclusions</h4>These findings display the significance of SARS-CoV2 monitoring to help on time detection of possible variants for pandemic control and vaccination plans. |
| first_indexed | 2024-12-12T01:24:31Z |
| format | Article |
| id | doaj.art-18c2f9b98b8747d4b41a9caaaca976d7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T01:24:31Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-18c2f9b98b8747d4b41a9caaaca976d72022-12-22T00:43:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01175e026784710.1371/journal.pone.0267847Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic.Jila YavarianAhmad NejatiVahid SalimiNazanin Zahra Shafiei JandaghiKaveh SadeghiAdel AbediAli Sharifi ZarchiMohammad Mehdi GouyaTalat Mokhtari-Azad<h4>Purpose</h4>Whole genome sequencing of SARS-CoV2 is important to find useful information about the viral lineages, variants of interests and variants of concern. As there are not enough data about the circulating SARS-CoV2 variants in Iran, we sequenced 54 SARS-CoV2 genomes during the 5 waves of pandemic in Iran.<h4>Methods</h4>After viral RNA extraction from clinical samples collected during the COVID-19 pandemic, next generation sequencing was performed using the Nextseq platform. The sequencing data were analyzed and compared with reference sequences.<h4>Results</h4>During the 1st wave, V and L clades were detected. The second wave was recognized by G, GH and GR clades. Circulating clades during the 3rd wave were GH and GR. In the fourth wave GRY (alpha variant), GK (delta variant) and one GH clade (beta variant) were detected. All viruses in the fifth wave were in clade GK (delta variant). There were different mutations in all parts of the genomes but Spike-D614G, NSP12-P323L, N-R203K and N-G204R were the most frequent mutants in these studied viruses.<h4>Conclusions</h4>These findings display the significance of SARS-CoV2 monitoring to help on time detection of possible variants for pandemic control and vaccination plans.https://doi.org/10.1371/journal.pone.0267847 |
| spellingShingle | Jila Yavarian Ahmad Nejati Vahid Salimi Nazanin Zahra Shafiei Jandaghi Kaveh Sadeghi Adel Abedi Ali Sharifi Zarchi Mohammad Mehdi Gouya Talat Mokhtari-Azad Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic. PLoS ONE |
| title | Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic. |
| title_full | Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic. |
| title_fullStr | Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic. |
| title_full_unstemmed | Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic. |
| title_short | Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic. |
| title_sort | whole genome sequencing of sars cov2 strains circulating in iran during five waves of pandemic |
| url | https://doi.org/10.1371/journal.pone.0267847 |
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