Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar rats
Liver damage is of a growing concern. The study aimed at investigating the effect of Nescafe on carbon tetrachloride (CCL4)-induced liver injury. Rats were randomly grouped viz; groups: 1 to 5 of 6 rats each. Groups-2 to 5 received intraperitoneal doses of CCl4, at 2ml/Kg body weight, in 3:1 v/v pr...
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| Format: | Article |
| Language: | English |
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Nigerian Medical Association, Akwa Ibom State Branch
2021-07-01
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| Series: | Ibom Medical Journal |
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| Online Access: | https://ibommedicaljournal.org/index.php/imjhome/article/view/53 |
| _version_ | 1797271857324359680 |
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| author | Chukwuedozie NF |
| author_facet | Chukwuedozie NF |
| author_sort | Chukwuedozie NF |
| collection | DOAJ |
| description |
Liver damage is of a growing concern. The study aimed at investigating the effect of Nescafe on carbon tetrachloride (CCL4)-induced liver injury. Rats were randomly grouped viz; groups: 1 to 5 of 6 rats each. Groups-2 to 5 received intraperitoneal doses of CCl4, at 2ml/Kg body weight, in 3:1 v/v preparation of CCL4 and olive oil, weekly for one month. Groups-1 and 2 were normal and positive control respectively and received distilled water (10ml/kg body water). Groups-3 to 5 were fed orally with 41.67, 62.22 and 72.22mg Nescafe suspension respectively, by gavage, weekly for one month. A day after treatment period, rats were anaesthetized and blood withdrawn from heart, using the heart puncture technique. Serum parameters viz: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein (TP), albumin, total bilirubin (TB), Total Cholesterol (T-Chol), Triglyceride, High Density Lipoprotein Cholesterol (HDL), Low density lipoprotein cholesterol (LDL) and Very Low-Density Lipoprotein cholesterol (VLDL) were studied. Group-2 activities of AST, ALT, ALP and concentrations of TB, globulin, T-Chol Triglyceride, LDL, VLDL, were increased while HDL, Albumin, TB concentrations were decreased from group-1. Aside from T-Chol and HDL, the changes were significant (P<0.05), compared to group-1. Group-3 through 5, the AST, ALT, ALP enzyme activities and globulin, TB, T-Chol, TAG, LDL VLDL concentrations decreased from Group-2. The TP, Albumin and HDL concentrations increased from group-2, ALT activities were significant (P<0.05), compared to group-1 while T-Cho and HDL were non-significant, compared to group-1. Results reviewed the protective effect of Nescafe on liver injury by CCl4.
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| first_indexed | 2024-03-07T14:16:13Z |
| format | Article |
| id | doaj.art-18c34a3eb1e5408790e60ebf9375cf00 |
| institution | Directory Open Access Journal |
| issn | 1597-7188 2735-9964 |
| language | English |
| last_indexed | 2024-03-07T14:16:13Z |
| publishDate | 2021-07-01 |
| publisher | Nigerian Medical Association, Akwa Ibom State Branch |
| record_format | Article |
| series | Ibom Medical Journal |
| spelling | doaj.art-18c34a3eb1e5408790e60ebf9375cf002024-03-06T12:03:18ZengNigerian Medical Association, Akwa Ibom State BranchIbom Medical Journal1597-71882735-99642021-07-0114310.61386/imj.v14i3.53Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar ratsChukwuedozie NF0Nigeria Police Academy, Department of Biochemistry and Forensic Science, Faculty of Science, P. M. B. 3474, Wudil, Kano State, Nigeria Liver damage is of a growing concern. The study aimed at investigating the effect of Nescafe on carbon tetrachloride (CCL4)-induced liver injury. Rats were randomly grouped viz; groups: 1 to 5 of 6 rats each. Groups-2 to 5 received intraperitoneal doses of CCl4, at 2ml/Kg body weight, in 3:1 v/v preparation of CCL4 and olive oil, weekly for one month. Groups-1 and 2 were normal and positive control respectively and received distilled water (10ml/kg body water). Groups-3 to 5 were fed orally with 41.67, 62.22 and 72.22mg Nescafe suspension respectively, by gavage, weekly for one month. A day after treatment period, rats were anaesthetized and blood withdrawn from heart, using the heart puncture technique. Serum parameters viz: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein (TP), albumin, total bilirubin (TB), Total Cholesterol (T-Chol), Triglyceride, High Density Lipoprotein Cholesterol (HDL), Low density lipoprotein cholesterol (LDL) and Very Low-Density Lipoprotein cholesterol (VLDL) were studied. Group-2 activities of AST, ALT, ALP and concentrations of TB, globulin, T-Chol Triglyceride, LDL, VLDL, were increased while HDL, Albumin, TB concentrations were decreased from group-1. Aside from T-Chol and HDL, the changes were significant (P<0.05), compared to group-1. Group-3 through 5, the AST, ALT, ALP enzyme activities and globulin, TB, T-Chol, TAG, LDL VLDL concentrations decreased from Group-2. The TP, Albumin and HDL concentrations increased from group-2, ALT activities were significant (P<0.05), compared to group-1 while T-Cho and HDL were non-significant, compared to group-1. Results reviewed the protective effect of Nescafe on liver injury by CCl4. https://ibommedicaljournal.org/index.php/imjhome/article/view/53CCL4 intoxicationliver injurylipid profileliver marker enzyme and liver function test |
| spellingShingle | Chukwuedozie NF Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar rats Ibom Medical Journal CCL4 intoxication liver injury lipid profile liver marker enzyme and liver function test |
| title | Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar rats |
| title_full | Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar rats |
| title_fullStr | Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar rats |
| title_full_unstemmed | Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar rats |
| title_short | Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar rats |
| title_sort | hepatoprotective effect of nescafe in carbon tetrachloride induced liver injury in wistar rats |
| topic | CCL4 intoxication liver injury lipid profile liver marker enzyme and liver function test |
| url | https://ibommedicaljournal.org/index.php/imjhome/article/view/53 |
| work_keys_str_mv | AT chukwuedozienf hepatoprotectiveeffectofnescafeincarbontetrachlorideinducedliverinjuryinwistarrats |