Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus <i>Endomelanconiopsis endophytica</i>
The demand for biodiesel worldwide is skyrocketing as the need to replace fossil diesel with renewable energy sources becomes increasingly pressing. In this context, biocatalysis is emerging as an environmentally friendly and highly efficient alternative to chemical catalysis. When combined with the...
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| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/16/19/6937 |
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| author | Juliana Gisele Corrêa Rodrigues Fernanda Veras Cardoso Celine Campos dos Santos Rosiane Rodrigues Matias Nélio Teixeira Machado Sergio Duvoisin Junior Patrícia Melchionna Albuquerque |
| author_facet | Juliana Gisele Corrêa Rodrigues Fernanda Veras Cardoso Celine Campos dos Santos Rosiane Rodrigues Matias Nélio Teixeira Machado Sergio Duvoisin Junior Patrícia Melchionna Albuquerque |
| author_sort | Juliana Gisele Corrêa Rodrigues |
| collection | DOAJ |
| description | The demand for biodiesel worldwide is skyrocketing as the need to replace fossil diesel with renewable energy sources becomes increasingly pressing. In this context, biocatalysis is emerging as an environmentally friendly and highly efficient alternative to chemical catalysis. When combined with the utilization of waste materials, it has the potential to make the process of biodiesel production sustainable. In the study, the potential of an extract rich in lipase produced by an Amazonian endophytic fungus as a biocatalyst in the transesterification of waste cooking oil for biodiesel production has been systematically investigated. The fungus <i>Endomelanconiopsis endophytica</i> exhibited an enzyme production of 11,262 U/mL after 120 h of cultivation. The lipolytic extract demonstrated its highest catalytic activity at 40 °C and a pH of 5.5. Using soybean oil and frying residue as raw materials, biodiesel was produced through biocatalytic transesterification, and yields of 91% and 89% (wt.), respectively, were achieved. By evaluating the process parameters, a maximum biodiesel yield of 90% was achieved using ethanol at a ratio of 3:1 ratio within 120 min. The experimental results demonstrate the feasibility and sustainability of applying a fungal enzymatic extract as a biocatalyst in the production of ethyl esters using waste cooking oil as a raw material. |
| first_indexed | 2024-03-10T21:45:24Z |
| format | Article |
| id | doaj.art-18c5e6fdb4d94094ab92c7e031babc43 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-10T21:45:24Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-18c5e6fdb4d94094ab92c7e031babc432023-11-19T14:20:57ZengMDPI AGEnergies1996-10732023-10-011619693710.3390/en16196937Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus <i>Endomelanconiopsis endophytica</i>Juliana Gisele Corrêa Rodrigues0Fernanda Veras Cardoso1Celine Campos dos Santos2Rosiane Rodrigues Matias3Nélio Teixeira Machado4Sergio Duvoisin Junior5Patrícia Melchionna Albuquerque6Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-010, BrazilGrupo de Pesquisa Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus 69050-020, BrazilGrupo de Pesquisa Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus 69050-020, BrazilGrupo de Pesquisa Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus 69050-020, BrazilFaculdade de Engenharia Sanitária e Ambiental, Universidade Federal do Pará, Belém 66075-910, BrazilGrupo de Pesquisa Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus 69050-020, BrazilPrograma de Pós-Graduação em Biodiversidade e Biotecnologia, Escola Superior de Ciências da Saúde, Universidade do Estado do Amazonas, Manaus 69050-010, BrazilThe demand for biodiesel worldwide is skyrocketing as the need to replace fossil diesel with renewable energy sources becomes increasingly pressing. In this context, biocatalysis is emerging as an environmentally friendly and highly efficient alternative to chemical catalysis. When combined with the utilization of waste materials, it has the potential to make the process of biodiesel production sustainable. In the study, the potential of an extract rich in lipase produced by an Amazonian endophytic fungus as a biocatalyst in the transesterification of waste cooking oil for biodiesel production has been systematically investigated. The fungus <i>Endomelanconiopsis endophytica</i> exhibited an enzyme production of 11,262 U/mL after 120 h of cultivation. The lipolytic extract demonstrated its highest catalytic activity at 40 °C and a pH of 5.5. Using soybean oil and frying residue as raw materials, biodiesel was produced through biocatalytic transesterification, and yields of 91% and 89% (wt.), respectively, were achieved. By evaluating the process parameters, a maximum biodiesel yield of 90% was achieved using ethanol at a ratio of 3:1 ratio within 120 min. The experimental results demonstrate the feasibility and sustainability of applying a fungal enzymatic extract as a biocatalyst in the production of ethyl esters using waste cooking oil as a raw material.https://www.mdpi.com/1996-1073/16/19/6937biocatalysis<i>Endomelanconiopsis endophytica</i>ethanoltransesterificationbiodiesel |
| spellingShingle | Juliana Gisele Corrêa Rodrigues Fernanda Veras Cardoso Celine Campos dos Santos Rosiane Rodrigues Matias Nélio Teixeira Machado Sergio Duvoisin Junior Patrícia Melchionna Albuquerque Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus <i>Endomelanconiopsis endophytica</i> Energies biocatalysis <i>Endomelanconiopsis endophytica</i> ethanol transesterification biodiesel |
| title | Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus <i>Endomelanconiopsis endophytica</i> |
| title_full | Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus <i>Endomelanconiopsis endophytica</i> |
| title_fullStr | Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus <i>Endomelanconiopsis endophytica</i> |
| title_full_unstemmed | Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus <i>Endomelanconiopsis endophytica</i> |
| title_short | Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus <i>Endomelanconiopsis endophytica</i> |
| title_sort | biocatalyzed transesterification of waste cooking oil for biodiesel production using lipase from the amazonian fungus i endomelanconiopsis endophytica i |
| topic | biocatalysis <i>Endomelanconiopsis endophytica</i> ethanol transesterification biodiesel |
| url | https://www.mdpi.com/1996-1073/16/19/6937 |
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