Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk
The impact of human milk (HM) or dairy milk-based formula (MF) on the large intestine’s metabolome was not investigated. Two-day old male piglets were randomly assigned to HM or MF diet (n = 26/group), from postnatal day (PND) 2 through 21 and weaned to a solid diet until PND 51. Piglets were euthan...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.607609/full |
| _version_ | 1818602125318422528 |
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| author | Fernanda Rosa Fernanda Rosa Katelin S. Matazel Katelin S. Matazel Anne K. Bowlin Keith D. Williams Keith D. Williams Ahmed A. Elolimy Ahmed A. Elolimy Sean H. Adams Sean H. Adams Lars Bode Lars Bode Laxmi Yeruva Laxmi Yeruva Laxmi Yeruva |
| author_facet | Fernanda Rosa Fernanda Rosa Katelin S. Matazel Katelin S. Matazel Anne K. Bowlin Keith D. Williams Keith D. Williams Ahmed A. Elolimy Ahmed A. Elolimy Sean H. Adams Sean H. Adams Lars Bode Lars Bode Laxmi Yeruva Laxmi Yeruva Laxmi Yeruva |
| author_sort | Fernanda Rosa |
| collection | DOAJ |
| description | The impact of human milk (HM) or dairy milk-based formula (MF) on the large intestine’s metabolome was not investigated. Two-day old male piglets were randomly assigned to HM or MF diet (n = 26/group), from postnatal day (PND) 2 through 21 and weaned to a solid diet until PND 51. Piglets were euthanized at PND 21 and PND 51, luminal contents of the cecum, proximal (PC) and distal colons (DC), and rectum were collected and subjected to metabolomics analysis. Data analyses were performed using Metaboanalyst. In comparison to MF, the HM diet resulted in higher levels of fatty acids in the lumen of the cecum, PC, DC, and rectum at PND 21. Glutamic acid was greater in the lumen of cecum, PC, and DC relative to the MF group at PND 21. Also, spermidine was higher in the DC and rectal contents of HM relative to MF at PND 21. MF diet resulted in greater abundances of amino acids in the cecal lumen relative to HM diet at PND 21. Additionally, several sugar metabolites were higher in various regions of the distal gut of MF fed piglets relative to HM group at PND 21. In contrast, at PND 51, in various regions there were higher levels of erythritol, maltotriose, isomaltose in HM versus MF fed piglets. This suggests a post weaning shift in sugar metabolism that is impacted by neonatal diet. The data also suggest that infant diet type and host-microbiota interactions likely influence the lower gut metabolome. |
| first_indexed | 2024-12-16T13:02:18Z |
| format | Article |
| id | doaj.art-18c6da217b0f4f1488176bda8fbe80d9 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-16T13:02:18Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-18c6da217b0f4f1488176bda8fbe80d92022-12-21T22:30:47ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-12-011110.3389/fimmu.2020.607609607609Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human MilkFernanda Rosa0Fernanda Rosa1Katelin S. Matazel2Katelin S. Matazel3Anne K. Bowlin4Keith D. Williams5Keith D. Williams6Ahmed A. Elolimy7Ahmed A. Elolimy8Sean H. Adams9Sean H. Adams10Lars Bode11Lars Bode12Laxmi Yeruva13Laxmi Yeruva14Laxmi Yeruva15Arkansas Children’s Nutrition Center, Little Rock, AR, United StatesDepartment of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United StatesArkansas Children’s Nutrition Center, Little Rock, AR, United StatesArkansas Children’s Research Institute, Little Rock, AR, United StatesDepartment of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, United StatesArkansas Children’s Nutrition Center, Little Rock, AR, United StatesDepartment of Biostatistics, University of Arkansas for Medical Sciences, Little Rock AR, United StatesArkansas Children’s Nutrition Center, Little Rock, AR, United StatesDepartment of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United StatesArkansas Children’s Nutrition Center, Little Rock, AR, United StatesDepartment of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United StatesLarsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence, University of California San Diego, La Jolla, CA, United StatesDepartment of Pediatrics, University of California San Diego, La Jolla, CA, United StatesArkansas Children’s Nutrition Center, Little Rock, AR, United StatesDepartment of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United StatesArkansas Children’s Research Institute, Little Rock, AR, United StatesThe impact of human milk (HM) or dairy milk-based formula (MF) on the large intestine’s metabolome was not investigated. Two-day old male piglets were randomly assigned to HM or MF diet (n = 26/group), from postnatal day (PND) 2 through 21 and weaned to a solid diet until PND 51. Piglets were euthanized at PND 21 and PND 51, luminal contents of the cecum, proximal (PC) and distal colons (DC), and rectum were collected and subjected to metabolomics analysis. Data analyses were performed using Metaboanalyst. In comparison to MF, the HM diet resulted in higher levels of fatty acids in the lumen of the cecum, PC, DC, and rectum at PND 21. Glutamic acid was greater in the lumen of cecum, PC, and DC relative to the MF group at PND 21. Also, spermidine was higher in the DC and rectal contents of HM relative to MF at PND 21. MF diet resulted in greater abundances of amino acids in the cecal lumen relative to HM diet at PND 21. Additionally, several sugar metabolites were higher in various regions of the distal gut of MF fed piglets relative to HM group at PND 21. In contrast, at PND 51, in various regions there were higher levels of erythritol, maltotriose, isomaltose in HM versus MF fed piglets. This suggests a post weaning shift in sugar metabolism that is impacted by neonatal diet. The data also suggest that infant diet type and host-microbiota interactions likely influence the lower gut metabolome.https://www.frontiersin.org/articles/10.3389/fimmu.2020.607609/fullhuman milkinfant formulaneonatesmetabolismhost-microbiota |
| spellingShingle | Fernanda Rosa Fernanda Rosa Katelin S. Matazel Katelin S. Matazel Anne K. Bowlin Keith D. Williams Keith D. Williams Ahmed A. Elolimy Ahmed A. Elolimy Sean H. Adams Sean H. Adams Lars Bode Lars Bode Laxmi Yeruva Laxmi Yeruva Laxmi Yeruva Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk Frontiers in Immunology human milk infant formula neonates metabolism host-microbiota |
| title | Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk |
| title_full | Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk |
| title_fullStr | Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk |
| title_full_unstemmed | Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk |
| title_short | Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk |
| title_sort | neonatal diet impacts the large intestine luminal metabolome at weaning and post weaning in piglets fed formula or human milk |
| topic | human milk infant formula neonates metabolism host-microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.607609/full |
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