First Colombian multicentric newborn screening for congenital toxoplasmosis.

AIMS: To determine the incidence of congenital toxoplasmosis in Colombian newborns from 19 hospital or maternal child health services from seven different cities of five natural geographic regions (Caribbean, Central, Andean, Amazonia and Eastern). MATERIALS AND METHODS: We collected 15,333 samples...

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Main Authors: Jorge Enrique Gómez-Marin, Alejandra de-la-Torre, Edith Angel-Muller, Jorge Rubio, Jaime Arenas, Elkin Osorio, Lilian Nuñez, Lyda Pinzon, Luis Carlos Mendez-Cordoba, Agustin Bustos, Isabel de-la-Hoz, Pedro Silva, Monica Beltran, Leonor Chacon, Martha Marrugo, Cristina Manjarres, Hernando Baquero, Fabiana Lora, Elizabeth Torres, Oscar Elias Zuluaga, Monica Estrada, Lacides Moscote, Myriam Teresa Silva, Raul Rivera, Angie Molina, Shirley Najera, Antonio Sanabria, Maria Luisa Ramirez, Claudia Alarcon, Natalia Restrepo, Alejandra Falla, Tailandia Rodriguez, Giovanny Castaño
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS Neglected Tropical Diseases
Online Access:http://europepmc.org/articles/PMC3104965?pdf=render
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author Jorge Enrique Gómez-Marin
Alejandra de-la-Torre
Edith Angel-Muller
Jorge Rubio
Jaime Arenas
Elkin Osorio
Lilian Nuñez
Lyda Pinzon
Luis Carlos Mendez-Cordoba
Agustin Bustos
Isabel de-la-Hoz
Pedro Silva
Monica Beltran
Leonor Chacon
Martha Marrugo
Cristina Manjarres
Hernando Baquero
Fabiana Lora
Elizabeth Torres
Oscar Elias Zuluaga
Monica Estrada
Lacides Moscote
Myriam Teresa Silva
Raul Rivera
Angie Molina
Shirley Najera
Antonio Sanabria
Maria Luisa Ramirez
Claudia Alarcon
Natalia Restrepo
Alejandra Falla
Tailandia Rodriguez
Giovanny Castaño
author_facet Jorge Enrique Gómez-Marin
Alejandra de-la-Torre
Edith Angel-Muller
Jorge Rubio
Jaime Arenas
Elkin Osorio
Lilian Nuñez
Lyda Pinzon
Luis Carlos Mendez-Cordoba
Agustin Bustos
Isabel de-la-Hoz
Pedro Silva
Monica Beltran
Leonor Chacon
Martha Marrugo
Cristina Manjarres
Hernando Baquero
Fabiana Lora
Elizabeth Torres
Oscar Elias Zuluaga
Monica Estrada
Lacides Moscote
Myriam Teresa Silva
Raul Rivera
Angie Molina
Shirley Najera
Antonio Sanabria
Maria Luisa Ramirez
Claudia Alarcon
Natalia Restrepo
Alejandra Falla
Tailandia Rodriguez
Giovanny Castaño
author_sort Jorge Enrique Gómez-Marin
collection DOAJ
description AIMS: To determine the incidence of congenital toxoplasmosis in Colombian newborns from 19 hospital or maternal child health services from seven different cities of five natural geographic regions (Caribbean, Central, Andean, Amazonia and Eastern). MATERIALS AND METHODS: We collected 15,333 samples from umbilical cord blood between the period of March 2009 to May 2010 in 19 different hospitals and maternal-child health services from seven different cities. We applied an IgM ELISA assay (Vircell, Spain) to determine the frequency of IgM anti Toxoplasma. The results in blood cord samples were confirmed either by western blot and repeated ELISA IgM assay. In a sub-sample of 1,613 children that were negative by the anti-Toxoplasma IgM assay, the frequency of specific anti-Toxoplasma IgA by the ISAGA assay was determined. All children with positive samples by IgM, IgA, clinical diagnosis or treatment during pregnancy were recalled for confirmatory tests after day 10 of life. RESULTS: 61 positive samples for specific IgM (0.39%) and 9 positives for IgA (0.5%) were found. 143 questionnaires were positive for a clinical diagnosis or treatment for toxoplasmosis during pregnancy. 109 out of the 218 children that had some of the criteria for postnatal confirmatory tests were followed. Congenital toxoplasmosis infection was confirmed in 15 children: 7 were symptomatic, and three of them died before the first month of life (20% of lethality). A significant correlation was found between a high incidence of markers for congenital toxoplasmosis and higher mean annual rainfall for the city. CONCLUSIONS: Incidence for congenital toxoplasmosis is significantly different between hospitals or maternal child health services from different cities in Colombia. Mean annual rainfall was correlated with incidence of congenital toxoplasmosis.
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spelling doaj.art-18c798647509498791b7c867e4c023962022-12-22T01:15:36ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352011-01-0155e119510.1371/journal.pntd.0001195First Colombian multicentric newborn screening for congenital toxoplasmosis.Jorge Enrique Gómez-MarinAlejandra de-la-TorreEdith Angel-MullerJorge RubioJaime ArenasElkin OsorioLilian NuñezLyda PinzonLuis Carlos Mendez-CordobaAgustin BustosIsabel de-la-HozPedro SilvaMonica BeltranLeonor ChaconMartha MarrugoCristina ManjarresHernando BaqueroFabiana LoraElizabeth TorresOscar Elias ZuluagaMonica EstradaLacides MoscoteMyriam Teresa SilvaRaul RiveraAngie MolinaShirley NajeraAntonio SanabriaMaria Luisa RamirezClaudia AlarconNatalia RestrepoAlejandra FallaTailandia RodriguezGiovanny CastañoAIMS: To determine the incidence of congenital toxoplasmosis in Colombian newborns from 19 hospital or maternal child health services from seven different cities of five natural geographic regions (Caribbean, Central, Andean, Amazonia and Eastern). MATERIALS AND METHODS: We collected 15,333 samples from umbilical cord blood between the period of March 2009 to May 2010 in 19 different hospitals and maternal-child health services from seven different cities. We applied an IgM ELISA assay (Vircell, Spain) to determine the frequency of IgM anti Toxoplasma. The results in blood cord samples were confirmed either by western blot and repeated ELISA IgM assay. In a sub-sample of 1,613 children that were negative by the anti-Toxoplasma IgM assay, the frequency of specific anti-Toxoplasma IgA by the ISAGA assay was determined. All children with positive samples by IgM, IgA, clinical diagnosis or treatment during pregnancy were recalled for confirmatory tests after day 10 of life. RESULTS: 61 positive samples for specific IgM (0.39%) and 9 positives for IgA (0.5%) were found. 143 questionnaires were positive for a clinical diagnosis or treatment for toxoplasmosis during pregnancy. 109 out of the 218 children that had some of the criteria for postnatal confirmatory tests were followed. Congenital toxoplasmosis infection was confirmed in 15 children: 7 were symptomatic, and three of them died before the first month of life (20% of lethality). A significant correlation was found between a high incidence of markers for congenital toxoplasmosis and higher mean annual rainfall for the city. CONCLUSIONS: Incidence for congenital toxoplasmosis is significantly different between hospitals or maternal child health services from different cities in Colombia. Mean annual rainfall was correlated with incidence of congenital toxoplasmosis.http://europepmc.org/articles/PMC3104965?pdf=render
spellingShingle Jorge Enrique Gómez-Marin
Alejandra de-la-Torre
Edith Angel-Muller
Jorge Rubio
Jaime Arenas
Elkin Osorio
Lilian Nuñez
Lyda Pinzon
Luis Carlos Mendez-Cordoba
Agustin Bustos
Isabel de-la-Hoz
Pedro Silva
Monica Beltran
Leonor Chacon
Martha Marrugo
Cristina Manjarres
Hernando Baquero
Fabiana Lora
Elizabeth Torres
Oscar Elias Zuluaga
Monica Estrada
Lacides Moscote
Myriam Teresa Silva
Raul Rivera
Angie Molina
Shirley Najera
Antonio Sanabria
Maria Luisa Ramirez
Claudia Alarcon
Natalia Restrepo
Alejandra Falla
Tailandia Rodriguez
Giovanny Castaño
First Colombian multicentric newborn screening for congenital toxoplasmosis.
PLoS Neglected Tropical Diseases
title First Colombian multicentric newborn screening for congenital toxoplasmosis.
title_full First Colombian multicentric newborn screening for congenital toxoplasmosis.
title_fullStr First Colombian multicentric newborn screening for congenital toxoplasmosis.
title_full_unstemmed First Colombian multicentric newborn screening for congenital toxoplasmosis.
title_short First Colombian multicentric newborn screening for congenital toxoplasmosis.
title_sort first colombian multicentric newborn screening for congenital toxoplasmosis
url http://europepmc.org/articles/PMC3104965?pdf=render
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