Optical Imaging Approaches to Investigating Radiation Resistance
Radiation therapy is frequently the first line of treatment for over 50% of cancer patients. While great advances have been made in improving treatment response rates and reducing damage to normal tissue, radiation resistance remains a persistent clinical problem. While hypoxia or a lack of tumor ox...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2019.01152/full |
| _version_ | 1828765779180388352 |
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| author | Sina Dadgar Narasimhan Rajaram |
| author_facet | Sina Dadgar Narasimhan Rajaram |
| author_sort | Sina Dadgar |
| collection | DOAJ |
| description | Radiation therapy is frequently the first line of treatment for over 50% of cancer patients. While great advances have been made in improving treatment response rates and reducing damage to normal tissue, radiation resistance remains a persistent clinical problem. While hypoxia or a lack of tumor oxygenation has long been considered a key factor in causing treatment failure, recent evidence points to metabolic reprogramming under well-oxygenated conditions as a potential route to promoting radiation resistance. In this review, we present recent studies from our lab and others that use high-resolution optical imaging as well as clinical translational optical spectroscopy to shine light on the biological basis of radiation resistance. Two-photon microscopy of endogenous cellular metabolism has identified key changes in both mitochondrial structure and function that are specific to radiation-resistant cells and help promote cell survival in response to radiation. Optical spectroscopic approaches, such as diffuse reflectance and Raman spectroscopy have demonstrated functional and molecular differences between radiation-resistant and sensitive tumors in response to radiation. These studies have uncovered key changes in metabolic pathways and present a viable route to clinical translation of optical technologies to determine radiation resistance at a very early stage in the clinic. |
| first_indexed | 2024-12-11T06:54:06Z |
| format | Article |
| id | doaj.art-18c81be5c78c4ddb8bc8b42077da584b |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-11T06:54:06Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-18c81be5c78c4ddb8bc8b42077da584b2022-12-22T01:16:49ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2019-11-01910.3389/fonc.2019.01152486839Optical Imaging Approaches to Investigating Radiation ResistanceSina DadgarNarasimhan RajaramRadiation therapy is frequently the first line of treatment for over 50% of cancer patients. While great advances have been made in improving treatment response rates and reducing damage to normal tissue, radiation resistance remains a persistent clinical problem. While hypoxia or a lack of tumor oxygenation has long been considered a key factor in causing treatment failure, recent evidence points to metabolic reprogramming under well-oxygenated conditions as a potential route to promoting radiation resistance. In this review, we present recent studies from our lab and others that use high-resolution optical imaging as well as clinical translational optical spectroscopy to shine light on the biological basis of radiation resistance. Two-photon microscopy of endogenous cellular metabolism has identified key changes in both mitochondrial structure and function that are specific to radiation-resistant cells and help promote cell survival in response to radiation. Optical spectroscopic approaches, such as diffuse reflectance and Raman spectroscopy have demonstrated functional and molecular differences between radiation-resistant and sensitive tumors in response to radiation. These studies have uncovered key changes in metabolic pathways and present a viable route to clinical translation of optical technologies to determine radiation resistance at a very early stage in the clinic.https://www.frontiersin.org/article/10.3389/fonc.2019.01152/fullraman spectroscopydiffuse reflectance spectroscopyoptical metabolic imagingnonlinear optical microscopymitochondrial organizationradiation resistance |
| spellingShingle | Sina Dadgar Narasimhan Rajaram Optical Imaging Approaches to Investigating Radiation Resistance Frontiers in Oncology raman spectroscopy diffuse reflectance spectroscopy optical metabolic imaging nonlinear optical microscopy mitochondrial organization radiation resistance |
| title | Optical Imaging Approaches to Investigating Radiation Resistance |
| title_full | Optical Imaging Approaches to Investigating Radiation Resistance |
| title_fullStr | Optical Imaging Approaches to Investigating Radiation Resistance |
| title_full_unstemmed | Optical Imaging Approaches to Investigating Radiation Resistance |
| title_short | Optical Imaging Approaches to Investigating Radiation Resistance |
| title_sort | optical imaging approaches to investigating radiation resistance |
| topic | raman spectroscopy diffuse reflectance spectroscopy optical metabolic imaging nonlinear optical microscopy mitochondrial organization radiation resistance |
| url | https://www.frontiersin.org/article/10.3389/fonc.2019.01152/full |
| work_keys_str_mv | AT sinadadgar opticalimagingapproachestoinvestigatingradiationresistance AT narasimhanrajaram opticalimagingapproachestoinvestigatingradiationresistance |