Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge.
Dogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because t...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4996460?pdf=render |
| _version_ | 1819080029617782784 |
|---|---|
| author | Lucilene Aparecida Resende Rodrigo Dian de Oliveira Aguiar-Soares Henrique Gama-Ker Bruno Mendes Roatt Ludmila Zanandreis de Mendonça Marina Luiza Rodrigues Alves Denise da Silveira-Lemos Rodrigo Corrêa-Oliveira Olindo Assis Martins-Filho Márcio Sobreira Silva Araújo Ricardo Toshio Fujiwara Nelder Figueiredo Gontijo Alexandre Barbosa Reis Rodolfo Cordeiro Giunchetti |
| author_facet | Lucilene Aparecida Resende Rodrigo Dian de Oliveira Aguiar-Soares Henrique Gama-Ker Bruno Mendes Roatt Ludmila Zanandreis de Mendonça Marina Luiza Rodrigues Alves Denise da Silveira-Lemos Rodrigo Corrêa-Oliveira Olindo Assis Martins-Filho Márcio Sobreira Silva Araújo Ricardo Toshio Fujiwara Nelder Figueiredo Gontijo Alexandre Barbosa Reis Rodolfo Cordeiro Giunchetti |
| author_sort | Lucilene Aparecida Resende |
| collection | DOAJ |
| description | Dogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because the sand fly salivary proteins are potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in past decades. We investigated the immunogenicity of the "LbSapSal" vaccine (L. braziliensis antigens, saponin as adjuvant, and Lutzomyia longipalpis salivary gland extract) in dogs at baseline (T0), during the post-vaccination protocol (T3rd) and after early (T90) and late (T885) times following L. chagasi-challenge. Our major data indicated that immunization with "LbSapSal" is able to induce biomarkers characterized by enhanced amounts of type I (tumor necrosis factor [TNF]-α, interleukin [IL]-12, interferon [IFN]-γ) cytokines and reduction in type II cytokines (IL-4 and TGF-β), even after experimental challenge. The establishment of a prominent pro-inflammatory immune response after "LbSapSal" immunization supported the increased levels of nitric oxide production, favoring a reduction in spleen parasitism (78.9%) and indicating long-lasting protection against L. chagasi infection. In conclusion, these results confirmed the hypothesis that the "LbSapSal" vaccination is a potential tool to control the Leishmania chagasi infection. |
| first_indexed | 2024-12-21T19:38:23Z |
| format | Article |
| id | doaj.art-18c902706d8d490b882feba67f9ae054 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T19:38:23Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-18c902706d8d490b882feba67f9ae0542022-12-21T18:52:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01118e016116910.1371/journal.pone.0161169Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge.Lucilene Aparecida ResendeRodrigo Dian de Oliveira Aguiar-SoaresHenrique Gama-KerBruno Mendes RoattLudmila Zanandreis de MendonçaMarina Luiza Rodrigues AlvesDenise da Silveira-LemosRodrigo Corrêa-OliveiraOlindo Assis Martins-FilhoMárcio Sobreira Silva AraújoRicardo Toshio FujiwaraNelder Figueiredo GontijoAlexandre Barbosa ReisRodolfo Cordeiro GiunchettiDogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because the sand fly salivary proteins are potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in past decades. We investigated the immunogenicity of the "LbSapSal" vaccine (L. braziliensis antigens, saponin as adjuvant, and Lutzomyia longipalpis salivary gland extract) in dogs at baseline (T0), during the post-vaccination protocol (T3rd) and after early (T90) and late (T885) times following L. chagasi-challenge. Our major data indicated that immunization with "LbSapSal" is able to induce biomarkers characterized by enhanced amounts of type I (tumor necrosis factor [TNF]-α, interleukin [IL]-12, interferon [IFN]-γ) cytokines and reduction in type II cytokines (IL-4 and TGF-β), even after experimental challenge. The establishment of a prominent pro-inflammatory immune response after "LbSapSal" immunization supported the increased levels of nitric oxide production, favoring a reduction in spleen parasitism (78.9%) and indicating long-lasting protection against L. chagasi infection. In conclusion, these results confirmed the hypothesis that the "LbSapSal" vaccination is a potential tool to control the Leishmania chagasi infection.http://europepmc.org/articles/PMC4996460?pdf=render |
| spellingShingle | Lucilene Aparecida Resende Rodrigo Dian de Oliveira Aguiar-Soares Henrique Gama-Ker Bruno Mendes Roatt Ludmila Zanandreis de Mendonça Marina Luiza Rodrigues Alves Denise da Silveira-Lemos Rodrigo Corrêa-Oliveira Olindo Assis Martins-Filho Márcio Sobreira Silva Araújo Ricardo Toshio Fujiwara Nelder Figueiredo Gontijo Alexandre Barbosa Reis Rodolfo Cordeiro Giunchetti Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. PLoS ONE |
| title | Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. |
| title_full | Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. |
| title_fullStr | Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. |
| title_full_unstemmed | Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. |
| title_short | Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge. |
| title_sort | impact of lbsapsal vaccine in canine immunological and parasitological features before and after leishmania chagasi challenge |
| url | http://europepmc.org/articles/PMC4996460?pdf=render |
| work_keys_str_mv | AT lucileneaparecidaresende impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT rodrigodiandeoliveiraaguiarsoares impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT henriquegamaker impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT brunomendesroatt impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT ludmilazanandreisdemendonca impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT marinaluizarodriguesalves impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT denisedasilveiralemos impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT rodrigocorreaoliveira impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT olindoassismartinsfilho impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT marciosobreirasilvaaraujo impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT ricardotoshiofujiwara impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT nelderfigueiredogontijo impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT alexandrebarbosareis impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge AT rodolfocordeirogiunchetti impactoflbsapsalvaccineincanineimmunologicalandparasitologicalfeaturesbeforeandafterleishmaniachagasichallenge |