Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity
The huge amount of intestinal bacteria represents a continuing threat to the intestinal barrier. To meet this challenge, gut epithelial cells produce antimicrobial peptides (AMP) that act at the forefront of innate immunity. We explore whether this antimicrobial activity and Paneth cells, the main i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00736/full |
| _version_ | 1818034949302779904 |
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| author | Silvia I. Cazorla Silvia I. Cazorla Carolina Maldonado-Galdeano Carolina Maldonado-Galdeano Ricardo Weill Juan De Paula Gabriela D. V. Perdigón Gabriela D. V. Perdigón |
| author_facet | Silvia I. Cazorla Silvia I. Cazorla Carolina Maldonado-Galdeano Carolina Maldonado-Galdeano Ricardo Weill Juan De Paula Gabriela D. V. Perdigón Gabriela D. V. Perdigón |
| author_sort | Silvia I. Cazorla |
| collection | DOAJ |
| description | The huge amount of intestinal bacteria represents a continuing threat to the intestinal barrier. To meet this challenge, gut epithelial cells produce antimicrobial peptides (AMP) that act at the forefront of innate immunity. We explore whether this antimicrobial activity and Paneth cells, the main intestinal cell responsible of AMP production, are influenced by probiotics administration, to avoid the imbalance of intestinal microbiota and preserve intestinal barrier. Administration of Lactobacillus casei CRL 431 (Lc 431) and L. paracasei CNCM I-1518 (Lp 1518) to 42 days old mice, increases the number of Paneth cells on small intestine, and the antimicrobial activity against the pathogens Staphylococcus aureus and Salmonella Typhimurium in the intestinal fluids. Specifically, strong damage of the bacterial cell with leakage of cytoplasmic content, and cellular fragmentation were observed in S. Typhimurium and S. aureus. Even more important, probiotics increase the antimicrobial activity of the intestinal fluids at the different ages, from weaning (21 days old) to old age (180 days old). Intestinal antimicrobial activity stimulated by oral probiotics, do not influence significantly the composition of total anaerobic bacteria, lactobacilli and enterobacteria in the large intestine, at any age analyzed. This result, together with the antimicrobial activity observed against the same probiotic bacteria; endorse the regular consumption of probiotics without adverse effect on the intestinal homeostasis in healthy individuals. We demonstrate that oral probiotics increase intestinal antimicrobial activity and Paneth cells in order to strengthen epithelial barrier against pathogens. This effect would be another important mechanism by which probiotics protect the host mainly against infectious diseases. |
| first_indexed | 2024-12-10T06:47:17Z |
| format | Article |
| id | doaj.art-18c920aa286843608be42d09f4094f1c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T06:47:17Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-18c920aa286843608be42d09f4094f1c2022-12-22T01:58:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-04-01910.3389/fmicb.2018.00736346002Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial ActivitySilvia I. Cazorla0Silvia I. Cazorla1Carolina Maldonado-Galdeano2Carolina Maldonado-Galdeano3Ricardo Weill4Juan De Paula5Gabriela D. V. Perdigón6Gabriela D. V. Perdigón7Laboratorio de Inmunología, Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán, ArgentinaCátedra de Inmunología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, ArgentinaLaboratorio de Inmunología, Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán, ArgentinaCátedra de Inmunología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, ArgentinaDepartamento de Investigación y Desarrollo, DANONE, Buenos Aires, ArgentinaServicio de Gastroenterología, Hospital Italiano de Buenos Aires, Buenos Aires, ArgentinaLaboratorio de Inmunología, Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán, ArgentinaCátedra de Inmunología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, ArgentinaThe huge amount of intestinal bacteria represents a continuing threat to the intestinal barrier. To meet this challenge, gut epithelial cells produce antimicrobial peptides (AMP) that act at the forefront of innate immunity. We explore whether this antimicrobial activity and Paneth cells, the main intestinal cell responsible of AMP production, are influenced by probiotics administration, to avoid the imbalance of intestinal microbiota and preserve intestinal barrier. Administration of Lactobacillus casei CRL 431 (Lc 431) and L. paracasei CNCM I-1518 (Lp 1518) to 42 days old mice, increases the number of Paneth cells on small intestine, and the antimicrobial activity against the pathogens Staphylococcus aureus and Salmonella Typhimurium in the intestinal fluids. Specifically, strong damage of the bacterial cell with leakage of cytoplasmic content, and cellular fragmentation were observed in S. Typhimurium and S. aureus. Even more important, probiotics increase the antimicrobial activity of the intestinal fluids at the different ages, from weaning (21 days old) to old age (180 days old). Intestinal antimicrobial activity stimulated by oral probiotics, do not influence significantly the composition of total anaerobic bacteria, lactobacilli and enterobacteria in the large intestine, at any age analyzed. This result, together with the antimicrobial activity observed against the same probiotic bacteria; endorse the regular consumption of probiotics without adverse effect on the intestinal homeostasis in healthy individuals. We demonstrate that oral probiotics increase intestinal antimicrobial activity and Paneth cells in order to strengthen epithelial barrier against pathogens. This effect would be another important mechanism by which probiotics protect the host mainly against infectious diseases.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00736/fullprobioticsantimicrobial activityPaneth cellsintestinal fluidsintestinal microbiota |
| spellingShingle | Silvia I. Cazorla Silvia I. Cazorla Carolina Maldonado-Galdeano Carolina Maldonado-Galdeano Ricardo Weill Juan De Paula Gabriela D. V. Perdigón Gabriela D. V. Perdigón Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity Frontiers in Microbiology probiotics antimicrobial activity Paneth cells intestinal fluids intestinal microbiota |
| title | Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity |
| title_full | Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity |
| title_fullStr | Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity |
| title_full_unstemmed | Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity |
| title_short | Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity |
| title_sort | oral administration of probiotics increases paneth cells and intestinal antimicrobial activity |
| topic | probiotics antimicrobial activity Paneth cells intestinal fluids intestinal microbiota |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00736/full |
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