Encapsulation of a <i>Desmodium intortum</i> Protein Isolate Pickering Emulsion of β-Carotene: Stability, Bioaccesibility and Cytotoxicity
Owing to their excellent characteristics, Pickering emulsions have been widely used in the development and the application of new carriers for embedding and for delivering active compounds. In this study, β-carotene was successfully encapsulated in a Pickering emulsion stabilized using <i>Desm...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/7/936 |
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| author | Xue-Mei Tang Pan-Dao Liu Zhi-Jian Chen Xin-Yong Li Rui Huang Guo-Dao Liu Rong-Shu Dong Jian Chen |
| author_facet | Xue-Mei Tang Pan-Dao Liu Zhi-Jian Chen Xin-Yong Li Rui Huang Guo-Dao Liu Rong-Shu Dong Jian Chen |
| author_sort | Xue-Mei Tang |
| collection | DOAJ |
| description | Owing to their excellent characteristics, Pickering emulsions have been widely used in the development and the application of new carriers for embedding and for delivering active compounds. In this study, β-carotene was successfully encapsulated in a Pickering emulsion stabilized using <i>Desmodium intortum</i> protein isolate (DIPI). The results showed that the encapsulation efficiencies of β-carotene in the control group Tween 20 emulsion (TE) and the DIPI Pickering emulsion (DIPIPE) were 46.7 ± 2.5% and 97.3 ± 0.8%, respectively. After storage for 30 days at 25 °C and 37 °C in a dark environment, approximately 79.4% and 72.1% of β-carotene in DIPIPE were retained. Compared with TE, DIPIPE can improve the stability of β-carotene during storage. In vitro digestion experiments showed that the bioaccessibility rate of β-carotene in DIPIPE was less than that in TE. Cytotoxicity experiments showed that DIPI and β-carotene micelles within a specific concentration range exerted no toxic effects on 3T3 cells. These results indicate that DIPIPE can be used as a good food-grade carrier for embedding and transporting active substances to broaden the application of the protein-based Pickering emulsion system in the development of functional foods. |
| first_indexed | 2024-03-09T11:51:08Z |
| format | Article |
| id | doaj.art-18d4a48edfd14fbeb919dbf1ff9ad9d8 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T11:51:08Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-18d4a48edfd14fbeb919dbf1ff9ad9d82023-11-30T23:14:42ZengMDPI AGFoods2304-81582022-03-0111793610.3390/foods11070936Encapsulation of a <i>Desmodium intortum</i> Protein Isolate Pickering Emulsion of β-Carotene: Stability, Bioaccesibility and CytotoxicityXue-Mei Tang0Pan-Dao Liu1Zhi-Jian Chen2Xin-Yong Li3Rui Huang4Guo-Dao Liu5Rong-Shu Dong6Jian Chen7Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, ChinaInstitute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, ChinaInstitute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, ChinaInstitute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, ChinaInstitute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, ChinaInstitute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, ChinaInstitute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, One Health Institute, Engineering Research Center of Utilization of Tropical Polysaccharide Resources, Ministry of Education, College of Food Science and Technology, Hainan University, Haikou 570228, ChinaOwing to their excellent characteristics, Pickering emulsions have been widely used in the development and the application of new carriers for embedding and for delivering active compounds. In this study, β-carotene was successfully encapsulated in a Pickering emulsion stabilized using <i>Desmodium intortum</i> protein isolate (DIPI). The results showed that the encapsulation efficiencies of β-carotene in the control group Tween 20 emulsion (TE) and the DIPI Pickering emulsion (DIPIPE) were 46.7 ± 2.5% and 97.3 ± 0.8%, respectively. After storage for 30 days at 25 °C and 37 °C in a dark environment, approximately 79.4% and 72.1% of β-carotene in DIPIPE were retained. Compared with TE, DIPIPE can improve the stability of β-carotene during storage. In vitro digestion experiments showed that the bioaccessibility rate of β-carotene in DIPIPE was less than that in TE. Cytotoxicity experiments showed that DIPI and β-carotene micelles within a specific concentration range exerted no toxic effects on 3T3 cells. These results indicate that DIPIPE can be used as a good food-grade carrier for embedding and transporting active substances to broaden the application of the protein-based Pickering emulsion system in the development of functional foods.https://www.mdpi.com/2304-8158/11/7/936Pickering emulsionβ-carotenesimulated in vitro digestionbioaccessibilitycytotoxicity |
| spellingShingle | Xue-Mei Tang Pan-Dao Liu Zhi-Jian Chen Xin-Yong Li Rui Huang Guo-Dao Liu Rong-Shu Dong Jian Chen Encapsulation of a <i>Desmodium intortum</i> Protein Isolate Pickering Emulsion of β-Carotene: Stability, Bioaccesibility and Cytotoxicity Foods Pickering emulsion β-carotene simulated in vitro digestion bioaccessibility cytotoxicity |
| title | Encapsulation of a <i>Desmodium intortum</i> Protein Isolate Pickering Emulsion of β-Carotene: Stability, Bioaccesibility and Cytotoxicity |
| title_full | Encapsulation of a <i>Desmodium intortum</i> Protein Isolate Pickering Emulsion of β-Carotene: Stability, Bioaccesibility and Cytotoxicity |
| title_fullStr | Encapsulation of a <i>Desmodium intortum</i> Protein Isolate Pickering Emulsion of β-Carotene: Stability, Bioaccesibility and Cytotoxicity |
| title_full_unstemmed | Encapsulation of a <i>Desmodium intortum</i> Protein Isolate Pickering Emulsion of β-Carotene: Stability, Bioaccesibility and Cytotoxicity |
| title_short | Encapsulation of a <i>Desmodium intortum</i> Protein Isolate Pickering Emulsion of β-Carotene: Stability, Bioaccesibility and Cytotoxicity |
| title_sort | encapsulation of a i desmodium intortum i protein isolate pickering emulsion of β carotene stability bioaccesibility and cytotoxicity |
| topic | Pickering emulsion β-carotene simulated in vitro digestion bioaccessibility cytotoxicity |
| url | https://www.mdpi.com/2304-8158/11/7/936 |
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