Improving the Way We See: Adaptive Optics Based Optical Microscopy for Deep-Tissue Imaging
With the recent developments in optical imaging tools and techniques, scientists are now able to image deeper regions of the tissue with greater resolution and accuracy. However, light scattering while imaging deeper regions of a biological tissue remains a fundamental issue. Presence of lipids, pro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Physics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphy.2021.654868/full |
| _version_ | 1818730589213163520 |
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| author | Pranoy Sahu Nirmal Mazumder |
| author_facet | Pranoy Sahu Nirmal Mazumder |
| author_sort | Pranoy Sahu |
| collection | DOAJ |
| description | With the recent developments in optical imaging tools and techniques, scientists are now able to image deeper regions of the tissue with greater resolution and accuracy. However, light scattering while imaging deeper regions of a biological tissue remains a fundamental issue. Presence of lipids, proteins and nucleic acids in the tissue makes it inhomogeneous for a given wavelength of light. Two-photon fluorescence (TPF) microscopy supplemented with improved invasive optical tools allows functional imaging in awake behaving mammals in an unprecedented manner. Similarly, improved optical methods conjugated with previously existing scanning laser ophthalmoscopy (SLO) has paved diffraction-limited retinal imaging. With the evolving technology, scientists are now able to resolve biological structures and function at the sub-cellular level. Wavefront correcting methods like adaptive optics (AO) has been implemented in correcting tissue or optical-based distortions, shaping the excitation beam in 3D-holography to target multiple neurons. And more recently, AO-based SLO is implemented for eye imaging both in research and clinical settings. In this review, we discuss some of the recent improvements in TPF microscopy with the application of AO for wavefront corrections and its recent application in brain imaging as well as ophthalmoscopy. |
| first_indexed | 2024-12-17T23:04:11Z |
| format | Article |
| id | doaj.art-18d545702c6b441da2c0a0fe8a6dd850 |
| institution | Directory Open Access Journal |
| issn | 2296-424X |
| language | English |
| last_indexed | 2024-12-17T23:04:11Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physics |
| spelling | doaj.art-18d545702c6b441da2c0a0fe8a6dd8502022-12-21T21:29:19ZengFrontiers Media S.A.Frontiers in Physics2296-424X2021-03-01910.3389/fphy.2021.654868654868Improving the Way We See: Adaptive Optics Based Optical Microscopy for Deep-Tissue ImagingPranoy Sahu0Nirmal Mazumder1Insitute of Biochemistry and Cell Biology, National Research Council, Naples, ItalyDepartment of Biophysics, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, IndiaWith the recent developments in optical imaging tools and techniques, scientists are now able to image deeper regions of the tissue with greater resolution and accuracy. However, light scattering while imaging deeper regions of a biological tissue remains a fundamental issue. Presence of lipids, proteins and nucleic acids in the tissue makes it inhomogeneous for a given wavelength of light. Two-photon fluorescence (TPF) microscopy supplemented with improved invasive optical tools allows functional imaging in awake behaving mammals in an unprecedented manner. Similarly, improved optical methods conjugated with previously existing scanning laser ophthalmoscopy (SLO) has paved diffraction-limited retinal imaging. With the evolving technology, scientists are now able to resolve biological structures and function at the sub-cellular level. Wavefront correcting methods like adaptive optics (AO) has been implemented in correcting tissue or optical-based distortions, shaping the excitation beam in 3D-holography to target multiple neurons. And more recently, AO-based SLO is implemented for eye imaging both in research and clinical settings. In this review, we discuss some of the recent improvements in TPF microscopy with the application of AO for wavefront corrections and its recent application in brain imaging as well as ophthalmoscopy.https://www.frontiersin.org/articles/10.3389/fphy.2021.654868/fulladaptive opticstwo-photon fluorescenceophthalmoscopytissue imagingbrain |
| spellingShingle | Pranoy Sahu Nirmal Mazumder Improving the Way We See: Adaptive Optics Based Optical Microscopy for Deep-Tissue Imaging Frontiers in Physics adaptive optics two-photon fluorescence ophthalmoscopy tissue imaging brain |
| title | Improving the Way We See: Adaptive Optics Based Optical Microscopy for Deep-Tissue Imaging |
| title_full | Improving the Way We See: Adaptive Optics Based Optical Microscopy for Deep-Tissue Imaging |
| title_fullStr | Improving the Way We See: Adaptive Optics Based Optical Microscopy for Deep-Tissue Imaging |
| title_full_unstemmed | Improving the Way We See: Adaptive Optics Based Optical Microscopy for Deep-Tissue Imaging |
| title_short | Improving the Way We See: Adaptive Optics Based Optical Microscopy for Deep-Tissue Imaging |
| title_sort | improving the way we see adaptive optics based optical microscopy for deep tissue imaging |
| topic | adaptive optics two-photon fluorescence ophthalmoscopy tissue imaging brain |
| url | https://www.frontiersin.org/articles/10.3389/fphy.2021.654868/full |
| work_keys_str_mv | AT pranoysahu improvingthewayweseeadaptiveopticsbasedopticalmicroscopyfordeeptissueimaging AT nirmalmazumder improvingthewayweseeadaptiveopticsbasedopticalmicroscopyfordeeptissueimaging |