Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells
In this study, we explore the behaviour of intracellular magnesium during bone phenotype modulation in a 3D cell model built to mimic osteogenesis. In addition, we measured the amount of magnesium in the mineral depositions generated during osteogenic induction. A two-fold increase of intracellular...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/7/2368 |
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| author | Giovanna Picone Concettina Cappadone Alice Pasini Joseph Lovecchio Marilisa Cortesi Giovanna Farruggia Marco Lombardo Alessandra Gianoncelli Lucia Mancini Menk Ralf H. Sandro Donato Emanuele Giordano Emil Malucelli Stefano Iotti |
| author_facet | Giovanna Picone Concettina Cappadone Alice Pasini Joseph Lovecchio Marilisa Cortesi Giovanna Farruggia Marco Lombardo Alessandra Gianoncelli Lucia Mancini Menk Ralf H. Sandro Donato Emanuele Giordano Emil Malucelli Stefano Iotti |
| author_sort | Giovanna Picone |
| collection | DOAJ |
| description | In this study, we explore the behaviour of intracellular magnesium during bone phenotype modulation in a 3D cell model built to mimic osteogenesis. In addition, we measured the amount of magnesium in the mineral depositions generated during osteogenic induction. A two-fold increase of intracellular magnesium content was found, both at three and seven days from the induction of differentiation. By X-ray microscopy, we characterized the morphology and chemical composition of the mineral depositions secreted by 3D cultured differentiated cells finding a marked co-localization of Mg with P at seven days of differentiation. This is the first experimental evidence on the presence of Mg in the mineral depositions generated during biomineralization, suggesting that Mg incorporation occurs during the bone forming process. In conclusion, this study on the one hand attests to an evident involvement of Mg in the process of cell differentiation, and, on the other hand, indicates that its multifaceted role needs further investigation. |
| first_indexed | 2024-03-10T20:48:33Z |
| format | Article |
| id | doaj.art-18d68fb5f36746e4872c7d90cdfe2f13 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:48:33Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-18d68fb5f36746e4872c7d90cdfe2f132023-11-19T20:07:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-03-01217236810.3390/ijms21072368Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 CellsGiovanna Picone0Concettina Cappadone1Alice Pasini2Joseph Lovecchio3Marilisa Cortesi4Giovanna Farruggia5Marco Lombardo6Alessandra Gianoncelli7Lucia Mancini8Menk Ralf H.9Sandro Donato10Emanuele Giordano11Emil Malucelli12Stefano Iotti13Department of Pharmacy and Biotechnology, University of Bologna, 33-40126 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 33-40126 Bologna, ItalyDepartment of Electrical, Electronic and Information Engineering “Guglielmo Marconi” (DEI), University of Bologna 50, 47522 Cesena, ItalyDepartment of Electrical, Electronic and Information Engineering “Guglielmo Marconi” (DEI), University of Bologna 50, 47522 Cesena, ItalyDepartment of Electrical, Electronic and Information Engineering “Guglielmo Marconi” (DEI), University of Bologna 50, 47522 Cesena, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 33-40126 Bologna, ItalyDepartment of Chemistry “G. Ciamician”, Alma Mater Studiorum–Università di Bologna, via Selmi 2, I-40126 Bologna, ItalyElettra-Sincrotrone Trieste S.C.p.A., Trieste, 34149 Basovizza, ItalyElettra-Sincrotrone Trieste S.C.p.A., Trieste, 34149 Basovizza, ItalyElettra-Sincrotrone Trieste S.C.p.A., Trieste, 34149 Basovizza, ItalyElettra-Sincrotrone Trieste S.C.p.A., Trieste, 34149 Basovizza, ItalyDepartment of Electrical, Electronic and Information Engineering “Guglielmo Marconi” (DEI), University of Bologna 50, 47522 Cesena, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 33-40126 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 33-40126 Bologna, ItalyIn this study, we explore the behaviour of intracellular magnesium during bone phenotype modulation in a 3D cell model built to mimic osteogenesis. In addition, we measured the amount of magnesium in the mineral depositions generated during osteogenic induction. A two-fold increase of intracellular magnesium content was found, both at three and seven days from the induction of differentiation. By X-ray microscopy, we characterized the morphology and chemical composition of the mineral depositions secreted by 3D cultured differentiated cells finding a marked co-localization of Mg with P at seven days of differentiation. This is the first experimental evidence on the presence of Mg in the mineral depositions generated during biomineralization, suggesting that Mg incorporation occurs during the bone forming process. In conclusion, this study on the one hand attests to an evident involvement of Mg in the process of cell differentiation, and, on the other hand, indicates that its multifaceted role needs further investigation.https://www.mdpi.com/1422-0067/21/7/2368osteogenesisosteoblastic differentiationosteosarcomabiomineralization |
| spellingShingle | Giovanna Picone Concettina Cappadone Alice Pasini Joseph Lovecchio Marilisa Cortesi Giovanna Farruggia Marco Lombardo Alessandra Gianoncelli Lucia Mancini Menk Ralf H. Sandro Donato Emanuele Giordano Emil Malucelli Stefano Iotti Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells International Journal of Molecular Sciences osteogenesis osteoblastic differentiation osteosarcoma biomineralization |
| title | Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells |
| title_full | Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells |
| title_fullStr | Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells |
| title_full_unstemmed | Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells |
| title_short | Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells |
| title_sort | analysis of intracellular magnesium and mineral depositions during osteogenic commitment of 3d cultured saos2 cells |
| topic | osteogenesis osteoblastic differentiation osteosarcoma biomineralization |
| url | https://www.mdpi.com/1422-0067/21/7/2368 |
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