Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativa
Camelina sativa (L.) Crantz an oilseed crop of the Brassicaceae family is gaining attention due to its potential as a source of high value oil for food, feed or fuel. The hexaploid domesticated C. sativa has limited genetic diversity, encouraging the exploration of related species for novel allelic...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2020-04-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.119.400957 |
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| author | Raju Chaudhary Chu Shin Koh Sateesh Kagale Lily Tang Siu Wah Wu Zhenling Lv Annaliese S. Mason Andrew G. Sharpe Axel Diederichsen Isobel A. P. Parkin |
| author_facet | Raju Chaudhary Chu Shin Koh Sateesh Kagale Lily Tang Siu Wah Wu Zhenling Lv Annaliese S. Mason Andrew G. Sharpe Axel Diederichsen Isobel A. P. Parkin |
| author_sort | Raju Chaudhary |
| collection | DOAJ |
| description | Camelina sativa (L.) Crantz an oilseed crop of the Brassicaceae family is gaining attention due to its potential as a source of high value oil for food, feed or fuel. The hexaploid domesticated C. sativa has limited genetic diversity, encouraging the exploration of related species for novel allelic variation for traits of interest. The current study utilized genotyping by sequencing to characterize 193 Camelina accessions belonging to seven different species collected primarily from the Ukrainian-Russian region and Eastern Europe. Population analyses among Camelina accessions with a 2n = 40 karyotype identified three subpopulations, two composed of domesticated C. sativa and one of C. microcarpa species. Winter type Camelina lines were identified as admixtures of C. sativa and C. microcarpa. Eighteen genotypes of related C. microcarpa unexpectedly shared only two subgenomes with C. sativa, suggesting a novel or cryptic sub-species of C. microcarpa with 19 haploid chromosomes. One C. microcarpa accession (2n = 26) was found to comprise the first two subgenomes of C. sativa suggesting a tetraploid structure. The defined chromosome series among C. microcarpa germplasm, including the newly designated C. neglecta diploid née C. microcarpa, suggested an evolutionary trajectory for the formation of the C. sativa hexaploid genome and re-defined the underlying subgenome structure of the reference genome. |
| first_indexed | 2024-12-17T02:21:40Z |
| format | Article |
| id | doaj.art-18d6c51140774a3193947d3c3c2cf9c4 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-17T02:21:40Z |
| publishDate | 2020-04-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-18d6c51140774a3193947d3c3c2cf9c42022-12-21T22:07:15ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362020-04-011041297130810.1534/g3.119.40095714Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativaRaju ChaudharyChu Shin KohSateesh KagaleLily TangSiu Wah WuZhenling LvAnnaliese S. MasonAndrew G. SharpeAxel DiederichsenIsobel A. P. ParkinCamelina sativa (L.) Crantz an oilseed crop of the Brassicaceae family is gaining attention due to its potential as a source of high value oil for food, feed or fuel. The hexaploid domesticated C. sativa has limited genetic diversity, encouraging the exploration of related species for novel allelic variation for traits of interest. The current study utilized genotyping by sequencing to characterize 193 Camelina accessions belonging to seven different species collected primarily from the Ukrainian-Russian region and Eastern Europe. Population analyses among Camelina accessions with a 2n = 40 karyotype identified three subpopulations, two composed of domesticated C. sativa and one of C. microcarpa species. Winter type Camelina lines were identified as admixtures of C. sativa and C. microcarpa. Eighteen genotypes of related C. microcarpa unexpectedly shared only two subgenomes with C. sativa, suggesting a novel or cryptic sub-species of C. microcarpa with 19 haploid chromosomes. One C. microcarpa accession (2n = 26) was found to comprise the first two subgenomes of C. sativa suggesting a tetraploid structure. The defined chromosome series among C. microcarpa germplasm, including the newly designated C. neglecta diploid née C. microcarpa, suggested an evolutionary trajectory for the formation of the C. sativa hexaploid genome and re-defined the underlying subgenome structure of the reference genome.http://g3journal.org/lookup/doi/10.1534/g3.119.400957camelinadomesticationcryptic speciesreference genomesubgenomerelated species |
| spellingShingle | Raju Chaudhary Chu Shin Koh Sateesh Kagale Lily Tang Siu Wah Wu Zhenling Lv Annaliese S. Mason Andrew G. Sharpe Axel Diederichsen Isobel A. P. Parkin Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativa G3: Genes, Genomes, Genetics camelina domestication cryptic species reference genome subgenome related species |
| title | Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativa |
| title_full | Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativa |
| title_fullStr | Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativa |
| title_full_unstemmed | Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativa |
| title_short | Assessing Diversity in the Camelina Genus Provides Insights into the Genome Structure of Camelina sativa |
| title_sort | assessing diversity in the camelina genus provides insights into the genome structure of camelina sativa |
| topic | camelina domestication cryptic species reference genome subgenome related species |
| url | http://g3journal.org/lookup/doi/10.1534/g3.119.400957 |
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