The Anti-apoptosis Effect of Single Electroacupuncture Treatment via Suppressing Neuronal Autophagy in the Acute Stage of Ischemic Stroke Without Infarct Alleviation
The main purpose of the study was to investigate the antiapoptotic effect of electroacupuncture (EA) in the acute stage of ischaemic stroke in rats. The cerebral ischemia model was established by middle cerebral artery occlusion (MCAO)/reperfusion in rats. A single EA treatment was performed at the...
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2021.633280/full |
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| author | Ying Xing Min Zhang Man-Man Wang Ya-Shuo Feng Fang Dong Feng Zhang Feng Zhang |
| author_facet | Ying Xing Min Zhang Man-Man Wang Ya-Shuo Feng Fang Dong Feng Zhang Feng Zhang |
| author_sort | Ying Xing |
| collection | DOAJ |
| description | The main purpose of the study was to investigate the antiapoptotic effect of electroacupuncture (EA) in the acute stage of ischaemic stroke in rats. The cerebral ischemia model was established by middle cerebral artery occlusion (MCAO)/reperfusion in rats. A single EA treatment was performed at the acute stage of ischaemic stroke. The neurological function, brain water content, apoptotic cell number, and cerebral infarct volume were assessed in stroke rats. The expression of autophagy-related proteins (LC3II/I, Beclin1, P62, and LAMP1), Sirtuin 1 (SIRT1), p-JNK, p-ERK1/2, and cleaved caspase-3 (CCAS3) were measured by Western blot, immunofluorescence, and immunohistochemistry. Rapamycin (RAP, an activator of autophagy) was used to confirm the antiapoptotic effect of EA via regulating autophagy. The brain edema infarct size and apoptotic cell number were increasing within 3 days following stroke, and brain edema reached its peak at 24 h after stroke. EA treatment at 24 h after ischaemic stroke obviously suppressed the number of apoptotic cells and brain edema. However, there were no significant differences in infarct volumes among EA-12 h, EA-24 h, and MCAO/R group. Moreover, EA treatment at 24 h after ischaemic stroke obviously suppressed the expression of CCAS3, LC3II/I, Beclin1 while increasing the level of P62 and LAMP1 and hence mediating autophagy, which was reversed by RAP. Meanwhile, the expression of SIRT1, p-ERK1/2, p-JNK were promoted by EA at 24 h after ischaemic stroke. In conclusion, EA treatment may suppress apoptosis possibly via regulating autophagy in the acute period after ischaemic stroke, hence reducing brain injury. |
| first_indexed | 2024-12-20T00:51:25Z |
| format | Article |
| id | doaj.art-18d9bd4f88d644ea93c471406853ad1a |
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| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-20T00:51:25Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-18d9bd4f88d644ea93c471406853ad1a2022-12-21T19:59:14ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022021-02-011510.3389/fncel.2021.633280633280The Anti-apoptosis Effect of Single Electroacupuncture Treatment via Suppressing Neuronal Autophagy in the Acute Stage of Ischemic Stroke Without Infarct AlleviationYing Xing0Min Zhang1Man-Man Wang2Ya-Shuo Feng3Fang Dong4Feng Zhang5Feng Zhang6Department of Rehabilitation Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Pathophysiology, Hebei Medical University, Shijiazhuang, ChinaDepartment of Rehabilitation Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Rehabilitation Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Clinical Laboratory Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Rehabilitation Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaHebei Key Laboratory of Critical Disease Mechanism and Intervention, Shijiazhuang, ChinaThe main purpose of the study was to investigate the antiapoptotic effect of electroacupuncture (EA) in the acute stage of ischaemic stroke in rats. The cerebral ischemia model was established by middle cerebral artery occlusion (MCAO)/reperfusion in rats. A single EA treatment was performed at the acute stage of ischaemic stroke. The neurological function, brain water content, apoptotic cell number, and cerebral infarct volume were assessed in stroke rats. The expression of autophagy-related proteins (LC3II/I, Beclin1, P62, and LAMP1), Sirtuin 1 (SIRT1), p-JNK, p-ERK1/2, and cleaved caspase-3 (CCAS3) were measured by Western blot, immunofluorescence, and immunohistochemistry. Rapamycin (RAP, an activator of autophagy) was used to confirm the antiapoptotic effect of EA via regulating autophagy. The brain edema infarct size and apoptotic cell number were increasing within 3 days following stroke, and brain edema reached its peak at 24 h after stroke. EA treatment at 24 h after ischaemic stroke obviously suppressed the number of apoptotic cells and brain edema. However, there were no significant differences in infarct volumes among EA-12 h, EA-24 h, and MCAO/R group. Moreover, EA treatment at 24 h after ischaemic stroke obviously suppressed the expression of CCAS3, LC3II/I, Beclin1 while increasing the level of P62 and LAMP1 and hence mediating autophagy, which was reversed by RAP. Meanwhile, the expression of SIRT1, p-ERK1/2, p-JNK were promoted by EA at 24 h after ischaemic stroke. In conclusion, EA treatment may suppress apoptosis possibly via regulating autophagy in the acute period after ischaemic stroke, hence reducing brain injury.https://www.frontiersin.org/articles/10.3389/fncel.2021.633280/fullstrokeautophagyapoptosiselectroacupunctureSIRT1JNK |
| spellingShingle | Ying Xing Min Zhang Man-Man Wang Ya-Shuo Feng Fang Dong Feng Zhang Feng Zhang The Anti-apoptosis Effect of Single Electroacupuncture Treatment via Suppressing Neuronal Autophagy in the Acute Stage of Ischemic Stroke Without Infarct Alleviation Frontiers in Cellular Neuroscience stroke autophagy apoptosis electroacupuncture SIRT1 JNK |
| title | The Anti-apoptosis Effect of Single Electroacupuncture Treatment via Suppressing Neuronal Autophagy in the Acute Stage of Ischemic Stroke Without Infarct Alleviation |
| title_full | The Anti-apoptosis Effect of Single Electroacupuncture Treatment via Suppressing Neuronal Autophagy in the Acute Stage of Ischemic Stroke Without Infarct Alleviation |
| title_fullStr | The Anti-apoptosis Effect of Single Electroacupuncture Treatment via Suppressing Neuronal Autophagy in the Acute Stage of Ischemic Stroke Without Infarct Alleviation |
| title_full_unstemmed | The Anti-apoptosis Effect of Single Electroacupuncture Treatment via Suppressing Neuronal Autophagy in the Acute Stage of Ischemic Stroke Without Infarct Alleviation |
| title_short | The Anti-apoptosis Effect of Single Electroacupuncture Treatment via Suppressing Neuronal Autophagy in the Acute Stage of Ischemic Stroke Without Infarct Alleviation |
| title_sort | anti apoptosis effect of single electroacupuncture treatment via suppressing neuronal autophagy in the acute stage of ischemic stroke without infarct alleviation |
| topic | stroke autophagy apoptosis electroacupuncture SIRT1 JNK |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2021.633280/full |
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