In vitro analysis of a local polymeric device as an alternative for systemic antibiotics in Dentistry
Abstract The development of a biodegradable material with antimicrobial properties for local applications is required in the prevention and treatment of infectious diseases. The objective of this study was to produce blends of poly-L-lactide acid (PLLA) synthetic polymer associated with several anti...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2017-12-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100279&lng=en&tlng=en |
| _version_ | 1818358861595148288 |
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| author | Talita Girio Carnaval Flávia Gonçalves Marcelo Munhóes Romano Luiz Henrique Catalani Marcia Alves Pinto Mayer Victor Elias Arana-Chávez Alexander Cassandri Nishida Thais Claudino Lage Carlos Eduardo Francci Carlos Alberto Adde |
| author_facet | Talita Girio Carnaval Flávia Gonçalves Marcelo Munhóes Romano Luiz Henrique Catalani Marcia Alves Pinto Mayer Victor Elias Arana-Chávez Alexander Cassandri Nishida Thais Claudino Lage Carlos Eduardo Francci Carlos Alberto Adde |
| author_sort | Talita Girio Carnaval |
| collection | DOAJ |
| description | Abstract The development of a biodegradable material with antimicrobial properties for local applications is required in the prevention and treatment of infectious diseases. The objective of this study was to produce blends of poly-L-lactide acid (PLLA) synthetic polymer associated with several antimicrobials, as an alternative in the prevention and treatment of infections, as well as to evaluate its cytotoxicity, release of antimicrobials and inhibit bacteria growth. Blends of PLLA added with 20% Amoxicillin, Metronidazole, Clindamycin or Azithromicyn were used to produce Films (F) or Meshs (M) by casting and electrospinning methods, respectively. Standardized discs of the films and meshs were stored in buffer solutions (pH 5 or 7.4) and aliquots were analyzed by high performance chromatography (HPLC) during 168 hours. Cytotoxicity on human gingival fibroblasts was tested after 24, 48 and 72h by MTT reaction. The antimicrobial capacity was determined against P. gingivalis and S. pyogenes. The specimens were weighed after 3 and 6 months of storage for degradation analysis. SEM was performed to control interfaces and degradation. Antimicrobials presented a continuous and exponential drug release. Analysis showed that both M and F were able to inhibit S. pyogenes and P. gingivalis growth, indicating the release of active antimicrobial agents. The products were not toxic to the fibroblasts. Amoxicillin-film showed more degradation than PLLA at both pHs (p < 0.05), whereas Azithromycin-meshes were more degraded than PLLA at pH 7.4 (p < 0.05). PLLA association with antimicrobials is biocompatible and may represent a potential tool for the local delivery of antimicrobials. |
| first_indexed | 2024-12-13T20:35:44Z |
| format | Article |
| id | doaj.art-18da9cfb8cc547968df1053ca25187e8 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-13T20:35:44Z |
| publishDate | 2017-12-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-18da9cfb8cc547968df1053ca25187e82022-12-21T23:32:18ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072017-12-0131010.1590/1807-3107bor-2017.vol31.0092S1806-83242017000100279In vitro analysis of a local polymeric device as an alternative for systemic antibiotics in DentistryTalita Girio CarnavalFlávia GonçalvesMarcelo Munhóes RomanoLuiz Henrique CatalaniMarcia Alves Pinto MayerVictor Elias Arana-ChávezAlexander Cassandri NishidaThais Claudino LageCarlos Eduardo FrancciCarlos Alberto AddeAbstract The development of a biodegradable material with antimicrobial properties for local applications is required in the prevention and treatment of infectious diseases. The objective of this study was to produce blends of poly-L-lactide acid (PLLA) synthetic polymer associated with several antimicrobials, as an alternative in the prevention and treatment of infections, as well as to evaluate its cytotoxicity, release of antimicrobials and inhibit bacteria growth. Blends of PLLA added with 20% Amoxicillin, Metronidazole, Clindamycin or Azithromicyn were used to produce Films (F) or Meshs (M) by casting and electrospinning methods, respectively. Standardized discs of the films and meshs were stored in buffer solutions (pH 5 or 7.4) and aliquots were analyzed by high performance chromatography (HPLC) during 168 hours. Cytotoxicity on human gingival fibroblasts was tested after 24, 48 and 72h by MTT reaction. The antimicrobial capacity was determined against P. gingivalis and S. pyogenes. The specimens were weighed after 3 and 6 months of storage for degradation analysis. SEM was performed to control interfaces and degradation. Antimicrobials presented a continuous and exponential drug release. Analysis showed that both M and F were able to inhibit S. pyogenes and P. gingivalis growth, indicating the release of active antimicrobial agents. The products were not toxic to the fibroblasts. Amoxicillin-film showed more degradation than PLLA at both pHs (p < 0.05), whereas Azithromycin-meshes were more degraded than PLLA at pH 7.4 (p < 0.05). PLLA association with antimicrobials is biocompatible and may represent a potential tool for the local delivery of antimicrobials.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100279&lng=en&tlng=enBiocompatible MaterialsPolymersantimicrobial, electrospinning |
| spellingShingle | Talita Girio Carnaval Flávia Gonçalves Marcelo Munhóes Romano Luiz Henrique Catalani Marcia Alves Pinto Mayer Victor Elias Arana-Chávez Alexander Cassandri Nishida Thais Claudino Lage Carlos Eduardo Francci Carlos Alberto Adde In vitro analysis of a local polymeric device as an alternative for systemic antibiotics in Dentistry Brazilian Oral Research Biocompatible Materials Polymers antimicrobial, electrospinning |
| title | In vitro analysis of a local polymeric device as an alternative for systemic antibiotics in Dentistry |
| title_full | In vitro analysis of a local polymeric device as an alternative for systemic antibiotics in Dentistry |
| title_fullStr | In vitro analysis of a local polymeric device as an alternative for systemic antibiotics in Dentistry |
| title_full_unstemmed | In vitro analysis of a local polymeric device as an alternative for systemic antibiotics in Dentistry |
| title_short | In vitro analysis of a local polymeric device as an alternative for systemic antibiotics in Dentistry |
| title_sort | in vitro analysis of a local polymeric device as an alternative for systemic antibiotics in dentistry |
| topic | Biocompatible Materials Polymers antimicrobial, electrospinning |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100279&lng=en&tlng=en |
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