Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke
The objective of the study was to determine antioxidant enzyme activity and concentration of malondialdehyde on broiler which consumed feed containing lauric acid and natural antioxidant from Areca vestiaria Giseke. The study used 240 day-old chicks of Lohmann MB 202 P strain placed randomly at 24 e...
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| Language: | English |
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Universitas Gadjah Mada
2018-05-01
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| Series: | Buletin Peternakan |
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| Online Access: | https://jurnal.ugm.ac.id/buletinpeternakan/article/view/31767 |
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| author | Jola Josephien Mariane Roosje Londok Sumiati Sumiati I Komang Gede Wiryawan Wasmen Manalu |
| author_facet | Jola Josephien Mariane Roosje Londok Sumiati Sumiati I Komang Gede Wiryawan Wasmen Manalu |
| author_sort | Jola Josephien Mariane Roosje Londok |
| collection | DOAJ |
| description | The objective of the study was to determine antioxidant enzyme activity and concentration of malondialdehyde on broiler which consumed feed containing lauric acid and natural antioxidant from Areca vestiaria Giseke. The study used 240 day-old chicks of Lohmann MB 202 P strain placed randomly at 24 experimental cage units (10 chickens each) of the litter system. The average temperature of the cage at the study was 25.80 to 32.080 C with relative air humidity rate of 67.69 to 86.20%. The research method followed a Factorial Completely Randomized Design 2 x 4 with 3 replications. The first factor was the source of lauric acid in feed, i.e. conventional coconut oil (CO) and pure lauric acid (LA). The second factor was the source of antioxidant in the form of dosage of Areca vestiaria (AV) and vitamin E supplementation (TF) with four levels of AV, ie 0, 625 mg.kg-1, 1250 mg.kg-1, and TF at a dose of 200 mg .kg-1 feed. Feed and drinking water were given ad libitum. Meat sampling to measure SOD, catalase and MDA was taken at the end of the study by first chickens were fasted for ± 8 hours (overnight). The sample used was right breast meat of experimental male chicken. The data were average from three experimental chickens. The data obtained were analyzed for the variant with the general linear model on MINITAB (version 16), then tested the differences between treatments using Tukey's honestly significant difference (HSD). The results showed that the source of laurate had a significant effect on SOD, catalase, and MDA, while antioxidant concentration significantly decreased SOD and MDA compared with control. The interaction between lauric sources and antioxidant concentrations affected catalase and MDA. The use of coconut oil as much as 3% and antioxidant supplementation of Areca vestiaria Giseke in feed at a dose of 1250 mg kg-1 could decrease lipid oxidation product of meat to produce a healthy food product. |
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| institution | Directory Open Access Journal |
| issn | 0126-4400 2407-876X |
| language | English |
| last_indexed | 2024-12-10T10:08:53Z |
| publishDate | 2018-05-01 |
| publisher | Universitas Gadjah Mada |
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| series | Buletin Peternakan |
| spelling | doaj.art-18dc8397a47e45de80ee977f374e04022022-12-22T01:53:10ZengUniversitas Gadjah MadaBuletin Peternakan0126-44002407-876X2018-05-0142210.21059/buletinpeternak.v42i2.3176720974Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria GisekeJola Josephien Mariane Roosje Londok0Sumiati Sumiati1I Komang Gede Wiryawan2Wasmen Manalu3Department of Animal Nutrition and Feed, Faculty of Animal Husbandry, Sam Ratulangi University, Manado, 95115 , IndonesiaDepartement of Nutrition and Feed Technology, Bogor Agricultural University, Bogor, 16680, IndonesiaDepartement of Nutrition and Feed Technology, Bogor Agricultural University, Bogor, 16680, IndonesiaDepartment of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, 16680, IndonesiaThe objective of the study was to determine antioxidant enzyme activity and concentration of malondialdehyde on broiler which consumed feed containing lauric acid and natural antioxidant from Areca vestiaria Giseke. The study used 240 day-old chicks of Lohmann MB 202 P strain placed randomly at 24 experimental cage units (10 chickens each) of the litter system. The average temperature of the cage at the study was 25.80 to 32.080 C with relative air humidity rate of 67.69 to 86.20%. The research method followed a Factorial Completely Randomized Design 2 x 4 with 3 replications. The first factor was the source of lauric acid in feed, i.e. conventional coconut oil (CO) and pure lauric acid (LA). The second factor was the source of antioxidant in the form of dosage of Areca vestiaria (AV) and vitamin E supplementation (TF) with four levels of AV, ie 0, 625 mg.kg-1, 1250 mg.kg-1, and TF at a dose of 200 mg .kg-1 feed. Feed and drinking water were given ad libitum. Meat sampling to measure SOD, catalase and MDA was taken at the end of the study by first chickens were fasted for ± 8 hours (overnight). The sample used was right breast meat of experimental male chicken. The data were average from three experimental chickens. The data obtained were analyzed for the variant with the general linear model on MINITAB (version 16), then tested the differences between treatments using Tukey's honestly significant difference (HSD). The results showed that the source of laurate had a significant effect on SOD, catalase, and MDA, while antioxidant concentration significantly decreased SOD and MDA compared with control. The interaction between lauric sources and antioxidant concentrations affected catalase and MDA. The use of coconut oil as much as 3% and antioxidant supplementation of Areca vestiaria Giseke in feed at a dose of 1250 mg kg-1 could decrease lipid oxidation product of meat to produce a healthy food product.https://jurnal.ugm.ac.id/buletinpeternakan/article/view/31767areca vestiaria gisekecatalaselauric acidmalondialdehydesuperoxide dismutase |
| spellingShingle | Jola Josephien Mariane Roosje Londok Sumiati Sumiati I Komang Gede Wiryawan Wasmen Manalu Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke Buletin Peternakan areca vestiaria giseke catalase lauric acid malondialdehyde superoxide dismutase |
| title | Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke |
| title_full | Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke |
| title_fullStr | Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke |
| title_full_unstemmed | Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke |
| title_short | Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke |
| title_sort | antioxidant enzyme activity and malondialdehyde concentration on broiler fed contain lauric acid and areca vestiaria giseke |
| topic | areca vestiaria giseke catalase lauric acid malondialdehyde superoxide dismutase |
| url | https://jurnal.ugm.ac.id/buletinpeternakan/article/view/31767 |
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