Virucidal efficacy of peracetic acid for instrument disinfection
Abstract Background Various peracetic-acid (PAA)-based products for processing flexible endoscopes on the market are often based on a two-component system including a cleaning step before the addition of PAA as disinfectant. The peracetic acid concentrations in these formulations from different manu...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | Antimicrobial Resistance and Infection Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13756-017-0271-3 |
| _version_ | 1819069721519063040 |
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| author | Britta Becker Florian H. H. Brill Daniel Todt Eike Steinmann Johannes Lenz Dajana Paulmann Birte Bischoff Jochen Steinmann |
| author_facet | Britta Becker Florian H. H. Brill Daniel Todt Eike Steinmann Johannes Lenz Dajana Paulmann Birte Bischoff Jochen Steinmann |
| author_sort | Britta Becker |
| collection | DOAJ |
| description | Abstract Background Various peracetic-acid (PAA)-based products for processing flexible endoscopes on the market are often based on a two-component system including a cleaning step before the addition of PAA as disinfectant. The peracetic acid concentrations in these formulations from different manufacturers are ranging from 400 to 1500 ppm (part per million). These products are used at temperatures between 20 °C and 37 °C. Since information on the virus-inactivating properties of peracetic acid at different concentrations and temperature is missing, it was the aim of the study to evaluate peracetic acid solutions against test viruses using the quantitative suspension test, EN 14476. In addition, further studies were performed with the recently established European pre norm (prEN 17111:2017) describing a carrier assay for simulating practical conditions using frosted glass. Methods In the first step of examination, different PAA solutions between 400 and 1500 ppm were tested at 20 °C, 25 °C, and 35 °C with three test viruses (adenovirus, murine norovirus and poliovirus) necessary for creating a virucidal action according to the European Norm, EN 14476. A second step for simulating practical conditions based on prEN 17111:2017 followed by spreading a test virus together with soil load onto a glass carrier which was immerged into a peracetic acid solution. A fixed exposure time of five minutes was used in all experiments. Results In the quantitative suspension test 1500 ppm PAA solution was needed at 35 °C for five minutes for the inactivation of poliovirus, whereas only 400 ppm at 20 °C for adeno- and murine norovirus were necessary. In the carrier assay 400 ppm peracetic acid at 20 °C were sufficient for adenovirus inactivation, whereas 600 ppm PAA were needed at 25 °C and 35 °C and 1000 ppm at 20 °C for murine norovirus. A PAA solution with 1000 ppm at 35 °C was required for complete inactivation of poliovirus. However, a dramatically decrease of titer after the drying and immerging could be observed. In consequence, a four log reduction of poliovirus titer could not be achieved in the carrier test. Conclusion In summary, 1500 ppm PAA at 35 °C was necessary for a virucidal action in the quantitative suspension test. After passing the requirements of the suspension test, additional examinations with adeno- and murine norovirus on glass carriers based on prEN 17111:2017 will not additionally contribute to the final claim of an instrument disinfectant for virucidal efficacy. This is due to the great stability of poliovirus in the preceded quantitative suspension test and the fact that poliovirus could not serve as test virus in the following carrier assay. |
| first_indexed | 2024-12-21T16:54:33Z |
| format | Article |
| id | doaj.art-18de96b39ecf4ef3a367bcee62581faf |
| institution | Directory Open Access Journal |
| issn | 2047-2994 |
| language | English |
| last_indexed | 2024-12-21T16:54:33Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
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| series | Antimicrobial Resistance and Infection Control |
| spelling | doaj.art-18de96b39ecf4ef3a367bcee62581faf2022-12-21T18:56:47ZengBMCAntimicrobial Resistance and Infection Control2047-29942017-11-01611610.1186/s13756-017-0271-3Virucidal efficacy of peracetic acid for instrument disinfectionBritta Becker0Florian H. H. Brill1Daniel Todt2Eike Steinmann3Johannes Lenz4Dajana Paulmann5Birte Bischoff6Jochen Steinmann7Dr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyDr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyInstitute for Experimental Virology, TWINCORE Centre for Experimental and Clinical Infection Research; a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI)Institute for Experimental Virology, TWINCORE Centre for Experimental and Clinical Infection Research; a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI)Chemische Fabrik Dr. Weigert GmbH & Co.KGDr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyDr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyDr. Brill + Partner GmbH Institute for Hygiene and MicrobiologyAbstract Background Various peracetic-acid (PAA)-based products for processing flexible endoscopes on the market are often based on a two-component system including a cleaning step before the addition of PAA as disinfectant. The peracetic acid concentrations in these formulations from different manufacturers are ranging from 400 to 1500 ppm (part per million). These products are used at temperatures between 20 °C and 37 °C. Since information on the virus-inactivating properties of peracetic acid at different concentrations and temperature is missing, it was the aim of the study to evaluate peracetic acid solutions against test viruses using the quantitative suspension test, EN 14476. In addition, further studies were performed with the recently established European pre norm (prEN 17111:2017) describing a carrier assay for simulating practical conditions using frosted glass. Methods In the first step of examination, different PAA solutions between 400 and 1500 ppm were tested at 20 °C, 25 °C, and 35 °C with three test viruses (adenovirus, murine norovirus and poliovirus) necessary for creating a virucidal action according to the European Norm, EN 14476. A second step for simulating practical conditions based on prEN 17111:2017 followed by spreading a test virus together with soil load onto a glass carrier which was immerged into a peracetic acid solution. A fixed exposure time of five minutes was used in all experiments. Results In the quantitative suspension test 1500 ppm PAA solution was needed at 35 °C for five minutes for the inactivation of poliovirus, whereas only 400 ppm at 20 °C for adeno- and murine norovirus were necessary. In the carrier assay 400 ppm peracetic acid at 20 °C were sufficient for adenovirus inactivation, whereas 600 ppm PAA were needed at 25 °C and 35 °C and 1000 ppm at 20 °C for murine norovirus. A PAA solution with 1000 ppm at 35 °C was required for complete inactivation of poliovirus. However, a dramatically decrease of titer after the drying and immerging could be observed. In consequence, a four log reduction of poliovirus titer could not be achieved in the carrier test. Conclusion In summary, 1500 ppm PAA at 35 °C was necessary for a virucidal action in the quantitative suspension test. After passing the requirements of the suspension test, additional examinations with adeno- and murine norovirus on glass carriers based on prEN 17111:2017 will not additionally contribute to the final claim of an instrument disinfectant for virucidal efficacy. This is due to the great stability of poliovirus in the preceded quantitative suspension test and the fact that poliovirus could not serve as test virus in the following carrier assay.http://link.springer.com/article/10.1186/s13756-017-0271-3Peracetic acidVirucidal efficacyInstrument disinfection |
| spellingShingle | Britta Becker Florian H. H. Brill Daniel Todt Eike Steinmann Johannes Lenz Dajana Paulmann Birte Bischoff Jochen Steinmann Virucidal efficacy of peracetic acid for instrument disinfection Antimicrobial Resistance and Infection Control Peracetic acid Virucidal efficacy Instrument disinfection |
| title | Virucidal efficacy of peracetic acid for instrument disinfection |
| title_full | Virucidal efficacy of peracetic acid for instrument disinfection |
| title_fullStr | Virucidal efficacy of peracetic acid for instrument disinfection |
| title_full_unstemmed | Virucidal efficacy of peracetic acid for instrument disinfection |
| title_short | Virucidal efficacy of peracetic acid for instrument disinfection |
| title_sort | virucidal efficacy of peracetic acid for instrument disinfection |
| topic | Peracetic acid Virucidal efficacy Instrument disinfection |
| url | http://link.springer.com/article/10.1186/s13756-017-0271-3 |
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