Incursion of Bluetongue in Tunisia: Molecular Characterization of Viral Strains
In December 1999, the bluetongue was declared in Tunisia. Other cases of the disease have also been reported in other countries in the Mediterranean basin. The aim of this work was to characterize viral isolates obtained during the epizootic, which occurred from December 1999 to March 2000, and to p...
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| Format: | Article |
| Language: | English |
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CIRAD
2003-03-01
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| Series: | Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux |
| Subjects: | |
| Online Access: | http://revues.cirad.fr/index.php/REMVT/article/view/9852 |
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| author | S. Ben Fredj E. Breard C. Sailleau S. Zientara S. Zekri A. Boudabbous S. Hammami |
| author_facet | S. Ben Fredj E. Breard C. Sailleau S. Zientara S. Zekri A. Boudabbous S. Hammami |
| author_sort | S. Ben Fredj |
| collection | DOAJ |
| description | In December 1999, the bluetongue was declared in Tunisia. Other cases of the disease have also been reported in other countries in the Mediterranean basin. The aim of this work was to characterize viral isolates obtained during the epizootic, which occurred from December 1999 to March 2000, and to perform serotype determination. The three genomic segments, 2, 7 and 10, corresponding to the proteins VP2, VP7 and NS3/NS3A, were amplified by PCR and sequenced. Sequences of the various genomic segments obtained from Tunisian viral isolates were compared with the Corsican vaccinal and wild-type strain, as well as with other bluetongue virus strains published in GenBank. The results are presented in the form of phylogenetic trees and tables in which nucleotide sequences are compared, thus showing the common origin of the Tunisian viral strain (serotype 2) and the Corsican strain that was responsible for the October 2000 epizootic. |
| first_indexed | 2024-03-12T09:05:26Z |
| format | Article |
| id | doaj.art-18e20a6e26d3440198aca59e378d719d |
| institution | Directory Open Access Journal |
| issn | 0035-1865 1951-6711 |
| language | English |
| last_indexed | 2024-03-12T09:05:26Z |
| publishDate | 2003-03-01 |
| publisher | CIRAD |
| record_format | Article |
| series | Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux |
| spelling | doaj.art-18e20a6e26d3440198aca59e378d719d2023-09-02T15:21:44ZengCIRADRevue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux0035-18651951-67112003-03-01563-412112710.19182/remvt.98529852Incursion of Bluetongue in Tunisia: Molecular Characterization of Viral StrainsS. Ben Fredj0E. Breard1C. Sailleau2S. Zientara3S. Zekri4A. Boudabbous5S. Hammami6Institut de la recherche vétérinaire de Tunisie, 14 rue Djebel Lakhdhar, la Rabta, 1006 Tunis, TunisieAgence française de sécurité sanitaire des aliments, 22 rue Pierre Curie, BP 67, 94703 Maisons-Alfort Cedex, FranceAgence française de sécurité sanitaire des aliments, 22 rue Pierre Curie, BP 67, 94703 Maisons-Alfort Cedex, FranceAgence française de sécurité sanitaire des aliments, 22 rue Pierre Curie, BP 67, 94703 Maisons-Alfort Cedex, FranceFaculté de médecine de Tunis, rue Djebel Lakhdhar, 1006 Tunis, TunisieFaculté des sciences mathématiques physiques et naturelles de Tunis, El Manar II, TunisieInstitut de la recherche vétérinaire de Tunisie, 14 rue Djebel Lakhdhar, la Rabta, 1006 Tunis, TunisieIn December 1999, the bluetongue was declared in Tunisia. Other cases of the disease have also been reported in other countries in the Mediterranean basin. The aim of this work was to characterize viral isolates obtained during the epizootic, which occurred from December 1999 to March 2000, and to perform serotype determination. The three genomic segments, 2, 7 and 10, corresponding to the proteins VP2, VP7 and NS3/NS3A, were amplified by PCR and sequenced. Sequences of the various genomic segments obtained from Tunisian viral isolates were compared with the Corsican vaccinal and wild-type strain, as well as with other bluetongue virus strains published in GenBank. The results are presented in the form of phylogenetic trees and tables in which nucleotide sequences are compared, thus showing the common origin of the Tunisian viral strain (serotype 2) and the Corsican strain that was responsible for the October 2000 epizootic.http://revues.cirad.fr/index.php/REMVT/article/view/9852OvinFièvre catarrhale du moutonVirus bluetongueBiologie moléculaireSéquence nucléotidiqueGénomeRégion méditerranéenneTunisie |
| spellingShingle | S. Ben Fredj E. Breard C. Sailleau S. Zientara S. Zekri A. Boudabbous S. Hammami Incursion of Bluetongue in Tunisia: Molecular Characterization of Viral Strains Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux Ovin Fièvre catarrhale du mouton Virus bluetongue Biologie moléculaire Séquence nucléotidique Génome Région méditerranéenne Tunisie |
| title | Incursion of Bluetongue in Tunisia: Molecular Characterization of Viral Strains |
| title_full | Incursion of Bluetongue in Tunisia: Molecular Characterization of Viral Strains |
| title_fullStr | Incursion of Bluetongue in Tunisia: Molecular Characterization of Viral Strains |
| title_full_unstemmed | Incursion of Bluetongue in Tunisia: Molecular Characterization of Viral Strains |
| title_short | Incursion of Bluetongue in Tunisia: Molecular Characterization of Viral Strains |
| title_sort | incursion of bluetongue in tunisia molecular characterization of viral strains |
| topic | Ovin Fièvre catarrhale du mouton Virus bluetongue Biologie moléculaire Séquence nucléotidique Génome Région méditerranéenne Tunisie |
| url | http://revues.cirad.fr/index.php/REMVT/article/view/9852 |
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