Effect of Acupuncture Treatment on Level of Serum Interferon (IFN)-γ in BALB/c Model of Leishmania Major Infection
Background: Cutaneous leishmaniasis (CL) is an endemic parasitic disease in Iran. Current treatments for the disease are not satisfying, have many side effects and are expensive. The healing effect of T-helper 1 (Th1) immune response, especially interferon (IFN)-γ secretion in CL has been previously...
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| Format: | Article |
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Isfahan University of Medical Sciences
2013-09-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/3056 |
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| author | Mahshid Shakibapour Shervin Ghaffari Hoseini Mohsen Mahmoodi Fatemeh Rostami Seyed Hossein Hejazi |
| author_facet | Mahshid Shakibapour Shervin Ghaffari Hoseini Mohsen Mahmoodi Fatemeh Rostami Seyed Hossein Hejazi |
| author_sort | Mahshid Shakibapour |
| collection | DOAJ |
| description | Background: Cutaneous leishmaniasis (CL) is an endemic parasitic disease in Iran. Current treatments for the disease are not satisfying, have many side effects and are expensive. The healing effect of T-helper 1 (Th1) immune response, especially interferon (IFN)-γ secretion in CL has been previously documented. It has been shown that acupuncture, a traditional Chinese medicine, also might activate Th1 immune response. In this study, the effect of acupuncture on serum level of IFN-γ in experimental CL of BALB/c mice was investigated.
Methods: 60 BALB/c mice were experimentally infected with Leishmania major and assigned randomly in to three groups: acupuncture treatment group which were given the acupuncture (thirty minutes daily, two days a week for 10 sessions) and intra-peritoneal diazepam, as a sedative agent; control group for diazepam which only received the diazepam; and a control group without any intervention. Serum level of IFN-γ was measured by means of ELISA at the beginning of the study and at sessions 5 and 10 of acupuncture treatment in three groups.
Findings: The mean serum level of IFN-γ was not significantly different between and within the groups at the beginning and at the sessions 5 and 10 of the therapy.
Conclusion: This investigation showed that acupuncture may not affect the serum level of IFN-γ in BALB/c model of CL. Moreover, diazepam does not interfere with the serum level of IFN-γ in such a study. Analysis of other immune factors and early measurement of IFN-γ in the course of treatment possibly may display the activated protective immune response against leishmaniasis by acupuncture. |
| first_indexed | 2024-03-12T09:11:32Z |
| format | Article |
| id | doaj.art-18e3dbb12ff54ffa8bd0ffc4132a974d |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:11:32Z |
| publishDate | 2013-09-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-18e3dbb12ff54ffa8bd0ffc4132a974d2023-09-02T14:58:04ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-09-0131245107710851380Effect of Acupuncture Treatment on Level of Serum Interferon (IFN)-γ in BALB/c Model of Leishmania Major InfectionMahshid Shakibapour0Shervin Ghaffari Hoseini1Mohsen Mahmoodi2Fatemeh Rostami3Seyed Hossein Hejazi4MSc Student, Department of Parasitology and Mycology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranInfectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Biology, School of Science, University of Isfahan, Isfahan, IranAssociate Professor, Skin Diseases and Leishmaniasis Research Center AND Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Cutaneous leishmaniasis (CL) is an endemic parasitic disease in Iran. Current treatments for the disease are not satisfying, have many side effects and are expensive. The healing effect of T-helper 1 (Th1) immune response, especially interferon (IFN)-γ secretion in CL has been previously documented. It has been shown that acupuncture, a traditional Chinese medicine, also might activate Th1 immune response. In this study, the effect of acupuncture on serum level of IFN-γ in experimental CL of BALB/c mice was investigated. Methods: 60 BALB/c mice were experimentally infected with Leishmania major and assigned randomly in to three groups: acupuncture treatment group which were given the acupuncture (thirty minutes daily, two days a week for 10 sessions) and intra-peritoneal diazepam, as a sedative agent; control group for diazepam which only received the diazepam; and a control group without any intervention. Serum level of IFN-γ was measured by means of ELISA at the beginning of the study and at sessions 5 and 10 of acupuncture treatment in three groups. Findings: The mean serum level of IFN-γ was not significantly different between and within the groups at the beginning and at the sessions 5 and 10 of the therapy. Conclusion: This investigation showed that acupuncture may not affect the serum level of IFN-γ in BALB/c model of CL. Moreover, diazepam does not interfere with the serum level of IFN-γ in such a study. Analysis of other immune factors and early measurement of IFN-γ in the course of treatment possibly may display the activated protective immune response against leishmaniasis by acupuncture.http://jims.mui.ac.ir/index.php/jims/article/view/3056LeishmaniasisAcupunctureLeishmania majorInterferon-γ |
| spellingShingle | Mahshid Shakibapour Shervin Ghaffari Hoseini Mohsen Mahmoodi Fatemeh Rostami Seyed Hossein Hejazi Effect of Acupuncture Treatment on Level of Serum Interferon (IFN)-γ in BALB/c Model of Leishmania Major Infection مجله دانشکده پزشکی اصفهان Leishmaniasis Acupuncture Leishmania major Interferon-γ |
| title | Effect of Acupuncture Treatment on Level of Serum Interferon (IFN)-γ in BALB/c Model of Leishmania Major Infection |
| title_full | Effect of Acupuncture Treatment on Level of Serum Interferon (IFN)-γ in BALB/c Model of Leishmania Major Infection |
| title_fullStr | Effect of Acupuncture Treatment on Level of Serum Interferon (IFN)-γ in BALB/c Model of Leishmania Major Infection |
| title_full_unstemmed | Effect of Acupuncture Treatment on Level of Serum Interferon (IFN)-γ in BALB/c Model of Leishmania Major Infection |
| title_short | Effect of Acupuncture Treatment on Level of Serum Interferon (IFN)-γ in BALB/c Model of Leishmania Major Infection |
| title_sort | effect of acupuncture treatment on level of serum interferon ifn γ in balb c model of leishmania major infection |
| topic | Leishmaniasis Acupuncture Leishmania major Interferon-γ |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/3056 |
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