Optimization of Process Conditions for Effective Degradation of Azo Blue Dye by Streptomyces DJP15
The present study was carried out to optimize the degradation process of textile azo blue dye by the potential strain Streptomyces DJP15 isolated from dye contaminated soil in and around Palakad Textile Industry, Palakad District, Kerala state, India. The decolourizing activity of the potential isol...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2017-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/optimization-of-process-conditions-for-effectivedegradation-of-azo-blue-dye-by-streptomyces-djp15/ |
| _version_ | 1818569699117498368 |
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| author | H.P. Jai Shanker Pillai |
| author_facet | H.P. Jai Shanker Pillai |
| author_sort | H.P. Jai Shanker Pillai |
| collection | DOAJ |
| description | The present study was carried out to optimize the degradation process of textile azo blue dye by the potential strain Streptomyces DJP15 isolated from dye contaminated soil in and around Palakad Textile Industry, Palakad District, Kerala state, India. The decolourizing activity of the potential isolate Streptomyces DJP15 was measured spectrophotomterically at every 6 h over a period of 54 h in starch casein broth amended with 50 mg/L of the test dye, azo blue. It was noticed that, there was a decrease in the optical density (OD) indicating the degradation of the test dye by the potential isolate Streptomyces DJP15. Different incubation conditions like shake condition, static condition, dye concentration, pH and temperature were used in the present study to investigate their effect on the rate of decolorisation. The potential isolate Streptomyces DJP15 exhibited significant decolourisation activity at 48 h of incubation for all the degradation condition studied. The conditions optimum found for degradation of the azo blue dye by the potential isolate Streptomyces DJP15. The highest degradations were noticed at static conditions, 50 mg/L of dye concentration, 3% v/v inoculum concentration, 7 pH and 35 °C temperature respectively. The results of the present study confirms that the isolate Streptomyces DJP15 was effective in degrading the textile dye azo blue under optimized conditions. |
| first_indexed | 2024-12-14T06:51:13Z |
| format | Article |
| id | doaj.art-18e56907d66a442584d5d1cc1303e713 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-14T06:51:13Z |
| publishDate | 2017-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-18e56907d66a442584d5d1cc1303e7132022-12-21T23:12:53ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-12-011141757176510.22207/JPAM.11.4.14Optimization of Process Conditions for Effective Degradation of Azo Blue Dye by Streptomyces DJP15H.P. Jai Shanker Pillai0Faculty of Public Health, St. Theresa International College, 1 Moo 6, Rangsit-Nakhonnayok Road (Klong 14), Bungsan, Ongkharak - 26210, Thailand.The present study was carried out to optimize the degradation process of textile azo blue dye by the potential strain Streptomyces DJP15 isolated from dye contaminated soil in and around Palakad Textile Industry, Palakad District, Kerala state, India. The decolourizing activity of the potential isolate Streptomyces DJP15 was measured spectrophotomterically at every 6 h over a period of 54 h in starch casein broth amended with 50 mg/L of the test dye, azo blue. It was noticed that, there was a decrease in the optical density (OD) indicating the degradation of the test dye by the potential isolate Streptomyces DJP15. Different incubation conditions like shake condition, static condition, dye concentration, pH and temperature were used in the present study to investigate their effect on the rate of decolorisation. The potential isolate Streptomyces DJP15 exhibited significant decolourisation activity at 48 h of incubation for all the degradation condition studied. The conditions optimum found for degradation of the azo blue dye by the potential isolate Streptomyces DJP15. The highest degradations were noticed at static conditions, 50 mg/L of dye concentration, 3% v/v inoculum concentration, 7 pH and 35 °C temperature respectively. The results of the present study confirms that the isolate Streptomyces DJP15 was effective in degrading the textile dye azo blue under optimized conditions.https://microbiologyjournal.org/optimization-of-process-conditions-for-effectivedegradation-of-azo-blue-dye-by-streptomyces-djp15/streptomycestextile dyeazo blueoptimizationbiodecolourisation. |
| spellingShingle | H.P. Jai Shanker Pillai Optimization of Process Conditions for Effective Degradation of Azo Blue Dye by Streptomyces DJP15 Journal of Pure and Applied Microbiology streptomyces textile dye azo blue optimization biodecolourisation. |
| title | Optimization of Process Conditions for Effective Degradation of Azo Blue Dye by Streptomyces DJP15 |
| title_full | Optimization of Process Conditions for Effective Degradation of Azo Blue Dye by Streptomyces DJP15 |
| title_fullStr | Optimization of Process Conditions for Effective Degradation of Azo Blue Dye by Streptomyces DJP15 |
| title_full_unstemmed | Optimization of Process Conditions for Effective Degradation of Azo Blue Dye by Streptomyces DJP15 |
| title_short | Optimization of Process Conditions for Effective Degradation of Azo Blue Dye by Streptomyces DJP15 |
| title_sort | optimization of process conditions for effective degradation of azo blue dye by streptomyces djp15 |
| topic | streptomyces textile dye azo blue optimization biodecolourisation. |
| url | https://microbiologyjournal.org/optimization-of-process-conditions-for-effectivedegradation-of-azo-blue-dye-by-streptomyces-djp15/ |
| work_keys_str_mv | AT hpjaishankerpillai optimizationofprocessconditionsforeffectivedegradationofazobluedyebystreptomycesdjp15 |