Overexpression ofapoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted micethan in apoB-100-only mice
The adaptive value of apolipoprotein B-48 (apoB-48), thetruncated form of apoB produced by the intestine, in lipid metabolism remainsunclear. We crossed human apoC-III transgenic mice with mice expressing eitherapoB-48 only (apoB48/48) or apoB-100 only(apoB100/100). Cholesterol levels were higher in...
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| Format: | Article |
| Language: | English |
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Elsevier
2004-12-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520341031 |
| _version_ | 1819072641675296768 |
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| author | Karin Conde-Knape Kenta Okada Rajasekhar Ramakrishnan NeilS. Shachter |
| author_facet | Karin Conde-Knape Kenta Okada Rajasekhar Ramakrishnan NeilS. Shachter |
| author_sort | Karin Conde-Knape |
| collection | DOAJ |
| description | The adaptive value of apolipoprotein B-48 (apoB-48), thetruncated form of apoB produced by the intestine, in lipid metabolism remainsunclear. We crossed human apoC-III transgenic mice with mice expressing eitherapoB-48 only (apoB48/48) or apoB-100 only(apoB100/100). Cholesterol levels were higher inapoB48/48 mice than in apoB100/100 micebut triglyceride levels were similar. Lipid levels were increased by the apoC-IIItransgene. However, triglyceride levels were significantly higher inapoB100/100C-III than in apoB48/48C-IIImice (895 ± 395 mg/dl vs. 690 ± 252 mg/dl; P <0.01), whereas cholesterol levels were higher in theapoB48/48C-III mice than inapoB100/100C-III (144 ± 35 mg/dl vs. 94 ± 30mg/dl; P < 0.00001). Triglyceride clearance from VLDL wasimpaired to a greater extent in apoB100/100C-III vs.apoB100/100 mice than in apoB48/48C-IIIvs. apoB48/48 mice. Triglyceride secretion rates were nodifferent in apoC-III transgenic mice than in their nontransgenic littermates.ApoB-48 triglyceride-rich lipoproteins were more resistant to thetriglyceride-increasing effects of apoC-III but appeared more sensitive to theremnant clearance inhibition.Our findings support a coordinated role forapoB-48 in facilitating the delivery of dietary triglycerides to the periphery.Consistent with such a mechanism, glucose levels were significantly higher inapoB48/48 mice vs. apoB100/100 mice,perhaps on the basis of metabolic competition. |
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| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T17:40:58Z |
| publishDate | 2004-12-01 |
| publisher | Elsevier |
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| series | Journal of Lipid Research |
| spelling | doaj.art-18e969c5ab2045febd5d3490248f486d2022-12-21T18:55:37ZengElsevierJournal of Lipid Research0022-22752004-12-01451222352244Overexpression ofapoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted micethan in apoB-100-only miceKarin Conde-Knape0Kenta Okada1Rajasekhar Ramakrishnan2NeilS. Shachter3Department of Medicine, Columbia University, New York, NY10032; Department of Pediatrics, Columbia University, NewYork, NY 10032Department of Medicine, Columbia University, New York, NY10032; Department of Pediatrics, Columbia University, NewYork, NY 10032Department of Medicine, Columbia University, New York, NY10032; Department of Pediatrics, Columbia University, NewYork, NY 10032Department of Medicine, Columbia University, New York, NY10032; Department of Pediatrics, Columbia University, NewYork, NY 10032The adaptive value of apolipoprotein B-48 (apoB-48), thetruncated form of apoB produced by the intestine, in lipid metabolism remainsunclear. We crossed human apoC-III transgenic mice with mice expressing eitherapoB-48 only (apoB48/48) or apoB-100 only(apoB100/100). Cholesterol levels were higher inapoB48/48 mice than in apoB100/100 micebut triglyceride levels were similar. Lipid levels were increased by the apoC-IIItransgene. However, triglyceride levels were significantly higher inapoB100/100C-III than in apoB48/48C-IIImice (895 ± 395 mg/dl vs. 690 ± 252 mg/dl; P <0.01), whereas cholesterol levels were higher in theapoB48/48C-III mice than inapoB100/100C-III (144 ± 35 mg/dl vs. 94 ± 30mg/dl; P < 0.00001). Triglyceride clearance from VLDL wasimpaired to a greater extent in apoB100/100C-III vs.apoB100/100 mice than in apoB48/48C-IIIvs. apoB48/48 mice. Triglyceride secretion rates were nodifferent in apoC-III transgenic mice than in their nontransgenic littermates.ApoB-48 triglyceride-rich lipoproteins were more resistant to thetriglyceride-increasing effects of apoC-III but appeared more sensitive to theremnant clearance inhibition.Our findings support a coordinated role forapoB-48 in facilitating the delivery of dietary triglycerides to the periphery.Consistent with such a mechanism, glucose levels were significantly higher inapoB48/48 mice vs. apoB100/100 mice,perhaps on the basis of metabolic competition.http://www.sciencedirect.com/science/article/pii/S0022227520341031apolipoproteins CapolipoproteinsBhyperglycemiatransgenicpostprandialperiod |
| spellingShingle | Karin Conde-Knape Kenta Okada Rajasekhar Ramakrishnan NeilS. Shachter Overexpression ofapoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted micethan in apoB-100-only mice Journal of Lipid Research apolipoproteins C apolipoproteinsB hyperglycemia transgenic postprandialperiod |
| title | Overexpression ofapoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted micethan in apoB-100-only mice |
| title_full | Overexpression ofapoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted micethan in apoB-100-only mice |
| title_fullStr | Overexpression ofapoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted micethan in apoB-100-only mice |
| title_full_unstemmed | Overexpression ofapoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted micethan in apoB-100-only mice |
| title_short | Overexpression ofapoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted micethan in apoB-100-only mice |
| title_sort | overexpression ofapoc iii produces lesser hypertriglyceridemia in apob 48 only gene targeted micethan in apob 100 only mice |
| topic | apolipoproteins C apolipoproteinsB hyperglycemia transgenic postprandialperiod |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520341031 |
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