Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes
A total of 180 serum samples (107 cows, 73 buffaloes) from cases of abortion and various reproductive disorders were collected for detection of Brucella antibody by Rose Bengal Plate Agglutination Test (RBPT), Serum Tube Agglutination Test (STAT) and indirect- ELISA (i-ELISA). The overall prevalence...
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| Format: | Article |
| Language: | English |
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Veterinary World
2010-04-01
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| Series: | Veterinary World |
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| Online Access: | http://www.scopemed.org/mnstemps/2/2-1297697795.pdf |
| _version_ | 1798028248012029952 |
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| author | S. N. Ghodasara Ashish Roy and B.B. Bhanderi |
| author_facet | S. N. Ghodasara Ashish Roy and B.B. Bhanderi |
| author_sort | S. N. Ghodasara |
| collection | DOAJ |
| description | A total of 180 serum samples (107 cows, 73 buffaloes) from cases of abortion and various reproductive disorders were collected for detection of Brucella antibody by Rose Bengal Plate Agglutination Test (RBPT), Serum Tube Agglutination Test (STAT) and indirect- ELISA (i-ELISA). The overall prevalence of brucellosis by RBPT, STAT and i-ELISA were 11.21%, 16.00% and 24.30% in cows 9.59%, 12.33% and 26.03% in buffaloes respectively. Overall seroprevalence of Brucellosis in cases of abortion, R.O.P. by RBPT, STAT and i-ELISA were 11.32%, 16.04% and 32.08% respectively. When three serological tests were compared, seropositivity was found highest by i-ELISA (25%), followed by STAT (14.45%) and RBPT (10.56%). The results shows higher prevalence of brucellosis in cases of abortion and R.O.P., while at lower level from various reproductive disorders as detected serologically indicating endemicity of the infection in villages around Anand city, Gujarat. [Vet. World 2010; 3(2.000): 61-64] |
| first_indexed | 2024-04-11T19:04:52Z |
| format | Article |
| id | doaj.art-18ed82b3ca034bb58314888390fc7b14 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 |
| language | English |
| last_indexed | 2024-04-11T19:04:52Z |
| publishDate | 2010-04-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-18ed82b3ca034bb58314888390fc7b142022-12-22T04:07:48ZengVeterinary WorldVeterinary World0972-89882010-04-0132.0006164Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and BuffaloesS. N. GhodasaraAshish Roy and B.B. BhanderiA total of 180 serum samples (107 cows, 73 buffaloes) from cases of abortion and various reproductive disorders were collected for detection of Brucella antibody by Rose Bengal Plate Agglutination Test (RBPT), Serum Tube Agglutination Test (STAT) and indirect- ELISA (i-ELISA). The overall prevalence of brucellosis by RBPT, STAT and i-ELISA were 11.21%, 16.00% and 24.30% in cows 9.59%, 12.33% and 26.03% in buffaloes respectively. Overall seroprevalence of Brucellosis in cases of abortion, R.O.P. by RBPT, STAT and i-ELISA were 11.32%, 16.04% and 32.08% respectively. When three serological tests were compared, seropositivity was found highest by i-ELISA (25%), followed by STAT (14.45%) and RBPT (10.56%). The results shows higher prevalence of brucellosis in cases of abortion and R.O.P., while at lower level from various reproductive disorders as detected serologically indicating endemicity of the infection in villages around Anand city, Gujarat. [Vet. World 2010; 3(2.000): 61-64]http://www.scopemed.org/mnstemps/2/2-1297697795.pdfBrucellaGynaecological disordersCowsBuffaloes |
| spellingShingle | S. N. Ghodasara Ashish Roy and B.B. Bhanderi Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes Veterinary World Brucella Gynaecological disorders Cows Buffaloes |
| title | Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes |
| title_full | Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes |
| title_fullStr | Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes |
| title_full_unstemmed | Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes |
| title_short | Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes |
| title_sort | comparison of rose bengal plate agglutination standard tube agglutination and indirect elisa tests for detection of brucella antibodies in cows and buffaloes |
| topic | Brucella Gynaecological disorders Cows Buffaloes |
| url | http://www.scopemed.org/mnstemps/2/2-1297697795.pdf |
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