MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSIS
Objective: To identify the morphological patterns of development and prevention of experimental epidural fibrosis based on the shape of the dural sac in the area of laminectomy. Methods: Male Wistar rats aged 4-5 months underwent laminectomy at the level of L6-S1. The animals were divided into tw...
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| Format: | Article |
| Language: | English |
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Avicenna Tajik State Medical University
2022-06-01
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| Series: | Паёми Сино |
| Subjects: | |
| Online Access: | https://doi.org/10.25005/2074-0581-2022-24-2-176-184 |
| _version_ | 1797805336673910784 |
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| author | I.A. SHURYGINA A.P. ZHIVOTENKO O.A. GOLDBERG V.A. SOROKOVIKOV S.N. LARIONOV |
| author_facet | I.A. SHURYGINA A.P. ZHIVOTENKO O.A. GOLDBERG V.A. SOROKOVIKOV S.N. LARIONOV |
| author_sort | I.A. SHURYGINA |
| collection | DOAJ |
| description | Objective: To identify the morphological patterns of development and prevention of experimental epidural fibrosis based on the shape of the dural sac in the area of laminectomy.
Methods: Male Wistar rats aged 4-5 months underwent laminectomy at the level of L6-S1. The animals were divided into two groups with 7 animals per group. In the main group (Group A) the animals were treated with Antiadhesin® gel applied to the wound to prevent the development of epidural fibrosis, while in the group of comparison (Group B), the gel was not applied. Another 7 healthy animals were used as a control group. The animals were taken out of experiment on the 28th day. Histological slides stained with hematoxylin-eosin were used to measure the width and length of the dural sac, its area, perimeter, and calculate its deformity index.
Results: In group B at the level of laminectomy, the ratio of the width to length of the dural sac was significantly higher than in the control group (p<0.05), mainly due to the enlarged width, while the length did not change significantly. In group A these parameters did not differ from the control group, which indicated that application of Antiadhesin® prevented formation of a connective tissue scar which could compress the dural sac and cause its deformation. In group A in the area of surgical intervention, granulomas with Pirogov-Langhans cells were detected, both in the soft tissues and in the lumen of the spinal canal. These findings in perioperative tissues might be related to biocompatibility and indicate their reaction to a gel as a foreign body.
Conclusion: In clinical practice optimal materials for the prevention of epidural fibrosis are missing. There is an obvious need for further research in order to obtain implants capable of inhibiting and reducing the formation of epidural fibrosis. |
| first_indexed | 2024-03-13T05:50:32Z |
| format | Article |
| id | doaj.art-18ee2654ae4d42dea08360e00085c033 |
| institution | Directory Open Access Journal |
| issn | 2074-0581 2959-6327 |
| language | English |
| last_indexed | 2024-03-13T05:50:32Z |
| publishDate | 2022-06-01 |
| publisher | Avicenna Tajik State Medical University |
| record_format | Article |
| series | Паёми Сино |
| spelling | doaj.art-18ee2654ae4d42dea08360e00085c0332023-06-13T13:18:41ZengAvicenna Tajik State Medical UniversityПаёми Сино2074-05812959-63272022-06-0124217618410.25005/2074-0581-2022-24-2-176-184MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSISI.A. SHURYGINA0A.P. ZHIVOTENKO1O.A. GOLDBERG2V.A. SOROKOVIKOV3S.N. LARIONOV4Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russian FederationIrkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russian FederationIrkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russian FederationIrkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russian Federation. Irkutsk State Medical Academy of Postgraduate Education – Branch Campus of the Russian Medical Academy of Continuing Professional Education, Irkutsk, Russian FederationIrkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russian FederationObjective: To identify the morphological patterns of development and prevention of experimental epidural fibrosis based on the shape of the dural sac in the area of laminectomy. Methods: Male Wistar rats aged 4-5 months underwent laminectomy at the level of L6-S1. The animals were divided into two groups with 7 animals per group. In the main group (Group A) the animals were treated with Antiadhesin® gel applied to the wound to prevent the development of epidural fibrosis, while in the group of comparison (Group B), the gel was not applied. Another 7 healthy animals were used as a control group. The animals were taken out of experiment on the 28th day. Histological slides stained with hematoxylin-eosin were used to measure the width and length of the dural sac, its area, perimeter, and calculate its deformity index. Results: In group B at the level of laminectomy, the ratio of the width to length of the dural sac was significantly higher than in the control group (p<0.05), mainly due to the enlarged width, while the length did not change significantly. In group A these parameters did not differ from the control group, which indicated that application of Antiadhesin® prevented formation of a connective tissue scar which could compress the dural sac and cause its deformation. In group A in the area of surgical intervention, granulomas with Pirogov-Langhans cells were detected, both in the soft tissues and in the lumen of the spinal canal. These findings in perioperative tissues might be related to biocompatibility and indicate their reaction to a gel as a foreign body. Conclusion: In clinical practice optimal materials for the prevention of epidural fibrosis are missing. There is an obvious need for further research in order to obtain implants capable of inhibiting and reducing the formation of epidural fibrosis.https://doi.org/10.25005/2074-0581-2022-24-2-176-184dura materepidural fibrosispreventionexperimentlaminectomyantiadhesin®. |
| spellingShingle | I.A. SHURYGINA A.P. ZHIVOTENKO O.A. GOLDBERG V.A. SOROKOVIKOV S.N. LARIONOV MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSIS Паёми Сино dura mater epidural fibrosis prevention experiment laminectomy antiadhesin®. |
| title | MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSIS |
| title_full | MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSIS |
| title_fullStr | MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSIS |
| title_full_unstemmed | MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSIS |
| title_short | MORPHOLOGICAL PATTERNS OF DEVELOPMENT AND PREVENTION OF EXPERIMENTAL EPIDURAL FIBROSIS |
| title_sort | morphological patterns of development and prevention of experimental epidural fibrosis |
| topic | dura mater epidural fibrosis prevention experiment laminectomy antiadhesin®. |
| url | https://doi.org/10.25005/2074-0581-2022-24-2-176-184 |
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